| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,864,336 | G→A | 16.7% | intergenic (+83/+65) | ygcP → / ← ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,864,336 | 0 | G | A | 16.7% | 225.0 / 32.9 | 96 | intergenic (+83/+65) | ygcP/ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Reads supporting (aligned to +/- strand): ref base G (51/28); new base A (6/10); total (57/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.39e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
CCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTG > minE/1864268‑1864406 | ccAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAc > 1:3527311/1‑71 (MQ=255) cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcg > 1:400592/1‑71 (MQ=255) aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgc < 1:840684/71‑1 (MQ=255) cgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCGGATGCGACGCTTAAcgc < 1:3529163‑M2/22‑1 (MQ=255) aTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgcg > 1:351447/1‑71 (MQ=255) aTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgcg > 1:603614/1‑71 (MQ=255) ttGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGt > 1:2226349/1‑71 (MQ=255) gtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGt > 1:1139882‑M2/48‑71 (MQ=255) tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa > 1:789276/1‑65 (MQ=255) gattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc > 1:2433217‑M2/46‑70 (MQ=255) gattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc > 1:1099010‑M2/46‑70 (MQ=255) attttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCt > 1:1342290‑M2/45‑70 (MQ=255) aaTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCt > 1:2518750/1‑70 (MQ=255) aTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTa > 1:3074421/1‑71 (MQ=255) tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:360383/1‑71 (MQ=255) tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:883636/1‑71 (MQ=255) ttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt < 1:2019613‑M2/29‑1 (MQ=255) tgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATc < 1:3765823‑M2/30‑1 (MQ=255) tGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:1662009/1‑69 (MQ=255) gtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCa < 1:1576870‑M2/31‑1 (MQ=255) gtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCa < 1:1235923‑M2/31‑1 (MQ=255) gtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCa < 1:2287383‑M2/31‑1 (MQ=255) taggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAgg < 1:2784971‑M2/33‑1 (MQ=255) taggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAg < 1:2708781‑M2/32‑1 (MQ=255) gTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAgg > 1:2157464/1‑71 (MQ=255) gTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg < 1:2989546/70‑1 (MQ=255) tttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGc < 1:3588221/71‑1 (MQ=255) tttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGc < 1:3592590/71‑1 (MQ=255) aggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGc > 1:1841386‑M2/38‑71 (MQ=255) ttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc > 1:1392949/1‑71 (MQ=255) tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc > 1:247178/1‑60 (MQ=255) tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc < 1:1587570/70‑1 (MQ=255) tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc > 1:2537266/1‑70 (MQ=255) tcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa > 1:1208360‑M2/35‑71 (MQ=255) tcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTa > 1:1896200‑M2/35‑71 (MQ=255) gATTTGTAGGCCGCACGTCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCt < 1:111699/70‑1 (MQ=255) cggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:287855‑M2/34‑62 (MQ=255) cggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc > 1:2838742‑M2/34‑71 (MQ=255) tttGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc < 1:2371164/70‑1 (MQ=255) ggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTacg < 1:2167983‑M2/39‑2 (MQ=255) ggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTacg > 1:1834397‑M2/33‑70 (MQ=255) gataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc < 1:3770279‑M2/40‑3 (MQ=255) gataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc < 1:3496256‑M2/40‑3 (MQ=255) ataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCctatc > 1:2039726‑M2/31‑67 (MQ=255) taaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc > 1:2336942‑M2/30‑67 (MQ=255) taaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCtacgc > 1:2597252‑M2/30‑67 (MQ=255) gTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAc > 1:1623181/1‑49 (MQ=255) gTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCa > 1:3770073/1‑60 (MQ=255) gTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCa > 1:1590822/1‑60 (MQ=255) aaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCtacgc > 1:2146674‑M2/29‑66 (MQ=255) ggCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTccc < 1:1629385/71‑1 (MQ=255) cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg > 1:732232/1‑55 (MQ=255) cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc > 1:3056204/1‑61 (MQ=255) cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCa > 1:2858827/1‑70 (MQ=255) tttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAAcgcg < 1:243830‑M2/23‑1 (MQ=255) tttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCa < 1:617188‑M2/31‑1 (MQ=255) tttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCcgcg < 1:1168714‑M2/23‑1 (MQ=255) gCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg > 1:2418114/1‑71 (MQ=255) cACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc > 1:734301/1‑71 (MQ=255) cACGCCACATCCGACATTCAGCGCCTGATGCGACGCTT‑ACGCGTCTTATCAGGCCTACAAGTCCCCAGGc < 1:2034244/70‑1 (MQ=255) cGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc > 1:2341777/1‑69 (MQ=255) cacaTCCGACATTCAGCGCCTGATGCGACGCTTGAcgc < 1:1811832/38‑1 (MQ=255) cacaTCCGACATTCAGCGCCTGATGCGACGCTTGAc < 1:2593669/36‑1 (MQ=255) cgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATc < 1:3845228‑M2/30‑1 (MQ=255) caTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg > 1:3739030/1‑63 (MQ=255) caTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAcc > 1:3650948/1‑71 (MQ=255) caTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAcc < 1:2492146/71‑1 (MQ=255) aTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg < 1:679890/71‑1 (MQ=255) tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGt < 1:1094792/71‑1 (MQ=255) atccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCctatc < 1:2678970‑M2/39‑3 (MQ=255) ccGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg > 1:2859123/1‑69 (MQ=255) ccGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg > 1:1193454/1‑69 (MQ=255) cGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAg < 1:1254918/71‑1 (MQ=255) cATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGCACCGTAgg > 1:3225905/1‑69 (MQ=255) cgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcat > 1:2132657/1‑71 (MQ=255) cgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcat > 1:1793332/1‑71 (MQ=255) gcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcat < 1:3569313/70‑1 (MQ=255) ccTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcatc < 1:3288067/70‑1 (MQ=255) tGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAg > 1:2222349/1‑57 (MQ=255) tGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGAATCATCCgg > 1:753821/1‑71 (MQ=255) aTGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa > 1:314985/1‑58 (MQ=255) aTGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa > 1:1529196/1‑58 (MQ=255) gCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAg > 1:3050439/1‑53 (MQ=255) cGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAaca > 1:673367/1‑71 (MQ=255) cGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac < 1:1981698/70‑1 (MQ=255) gACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAgg < 1:2369542/56‑1 (MQ=255) aCGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACg > 1:3018643/1‑71 (MQ=255) cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGat > 1:718480/1‑56 (MQ=255) cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt < 1:1460937/71‑1 (MQ=255) cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt > 1:1096374/1‑71 (MQ=255) gCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcatc > 1:1955922/1‑59 (MQ=255) gCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGtt < 1:1230760/71‑1 (MQ=255) cTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGtt < 1:1915051/70‑1 (MQ=255) cTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTa < 1:3132409/71‑1 (MQ=255) ttGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTAt > 1:1958384/1‑71 (MQ=255) tGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTAtt > 1:2900330/1‑71 (MQ=255) gACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTAtt < 1:2168834/70‑1 (MQ=255) gACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTAtt < 1:2041684/70‑1 (MQ=255) gACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTg > 1:2323090/1‑71 (MQ=255) gACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGCATCATCCGGCAACACGTTAt < 1:3271461/69‑1 (MQ=255) | CCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTG > minE/1864268‑1864406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |