| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,873,630 | C→A | 38.0% | intergenic (+774/+599) | ygcE → / ← ygcF | predicted kinase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,873,630 | 0 | C | A | 38.0% | 22.1 / 13.7 | 21 | intergenic (+774/+599) | ygcE/ygcF | predicted kinase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (7/6); new base A (5/3); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
GCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCG > minE/1873568‑1873698 | gCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCgg > 1:3425924/1‑71 (MQ=255) cgcgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAac < 1:573945/71‑1 (MQ=255) cgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAacac < 1:2474164/71‑1 (MQ=255) gggAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGGTTATCCCCGCTGGCGCGGGGAACACgg < 1:645242/71‑1 (MQ=255) aaCACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACGGgcg > 1:414778/1‑71 (MQ=255) cacCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCa > 1:237225/1‑71 (MQ=255) acCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCAc > 1:3322285/1‑71 (MQ=255) cTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACg > 1:187127/1‑70 (MQ=255) aaTCTATGGACTATTGCTATTCGGTTTATCCCCGCTg > 1:2545313/1‑37 (MQ=255) aaTCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGcc > 1:515874/1‑71 (MQ=255) aTCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCg > 1:3605298/1‑71 (MQ=255) gACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCt < 1:956894/71‑1 (MQ=255) aCTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTg < 1:27050/71‑1 (MQ=255) ttGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTc < 1:285464/70‑1 (MQ=255) tATTCGGTTTATCCCCGATGGCGCGGGGAACACGGgcg > 1:846845/1‑38 (MQ=255) ttCGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTAt > 1:1601538/1‑58 (MQ=255) gTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATccc > 1:2197886/1‑70 (MQ=255) tttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTg < 1:2471406/56‑1 (MQ=255) tATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGc < 1:2925278/70‑1 (MQ=255) tATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGc < 1:3488144/70‑1 (MQ=255) cGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGcgcg > 1:3590826/1‑71 (MQ=255) | GCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCG > minE/1873568‑1873698 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |