| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,889,569 | A→G | 9.1% | R220R (CGT→CGC) | gudX ← | predicted glucarate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,889,569 | 0 | A | G | 9.1% | 214.1 / 10.3 | 77 | R220R (CGT→CGC) | gudX | predicted glucarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (26/44); new base G (3/4); total (29/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGC > minE/1889501‑1889639 | tcatcaAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCAc > 1:1758505/1‑70 (MQ=255) catcaAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCAc < 1:2290959/69‑1 (MQ=255) catcaAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCAc < 1:3461961/69‑1 (MQ=255) catcaAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACg > 1:950165/1‑70 (MQ=255) atcaAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGaa > 1:1733211/1‑71 (MQ=255) aaGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACa > 1:530792/1‑70 (MQ=255) aaGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAg > 1:1648406/1‑71 (MQ=255) gcagcCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAgtg > 1:3132193/1‑71 (MQ=255) gcagcCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAgtg < 1:3711579/71‑1 (MQ=255) gcagcCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAgtg < 1:3361247/71‑1 (MQ=255) cagcCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAgtgt < 1:2647745/71‑1 (MQ=255) cagcCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAgtgt < 1:2307342/71‑1 (MQ=255) tGCACCGTTGGTATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAttt < 1:1846975/71‑1 (MQ=255) tGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCa > 1:1369443/1‑55 (MQ=255) tGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAttt > 1:3212975/1‑71 (MQ=255) gCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCGCGAACAGTGTCGATTTc > 1:1426352/1‑71 (MQ=255) ccGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCGCGAACAGTGTCGATTTCTTg < 1:1146290/71‑1 (MQ=255) ccGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCGCGAACAGTGTCGATTTCTTg < 1:334975/71‑1 (MQ=255) cGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGa > 1:3863885/1‑63 (MQ=255) cGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGa > 1:847229/1‑63 (MQ=255) cGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGc > 1:157702/1‑71 (MQ=255) ttGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGaa < 1:1558663/52‑1 (MQ=255) ggATCAACGGTAATCCGCGCATCCGTGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGcc < 1:658858/71‑1 (MQ=255) ggATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGcc < 1:611757/71‑1 (MQ=255) ggATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGcc < 1:2271177/71‑1 (MQ=255) gATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGc > 1:3170060/1‑69 (MQ=255) aTCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCGCGAACAGTGTCGATTTc < 1:1780179/60‑1 (MQ=255) tCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACa > 1:3093609/1‑48 (MQ=255) tCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAgg > 1:2614518/1‑71 (MQ=255) aaCGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCGCGAACAGTGTCGATTTCTTGCTCGcc > 1:827065/1‑66 (MQ=255) aaCGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAg < 1:1364481/68‑1 (MQ=255) cGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAAc < 1:1068836/43‑1 (MQ=255) cGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTc > 1:1611609/1‑49 (MQ=255) cGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTaa > 1:1425985/1‑70 (MQ=255) cGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTa > 1:2273621/1‑69 (MQ=255) tAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACAcg > 1:2505629/1‑71 (MQ=255) tCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAtt < 1:3041518/47‑1 (MQ=255) tCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAtt < 1:2931885/47‑1 (MQ=255) tCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACAcgc < 1:1262324/69‑1 (MQ=255) tCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACAcgc < 1:2661255/69‑1 (MQ=255) ccGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACAcgccg < 1:1860804/70‑1 (MQ=255) gcgcATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTc > 1:711628/1‑52 (MQ=255) tCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAt < 1:2085924/38‑1 (MQ=255) tCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAt < 1:2600078/38‑1 (MQ=255) tCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTa < 1:607122/55‑1 (MQ=255) ccGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGc < 1:3403939/48‑1 (MQ=255) gAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCtt > 1:597221/1‑38 (MQ=255) aaGCGTTTCTTCAATGCACGAACAGTGTCGATTTCt < 1:2878002/36‑1 (MQ=255) aaGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGt < 1:1379573/48‑1 (MQ=255) gcttttCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTTAAAt > 1:1755336/4‑71 (MQ=255) gTTTCTTCAATGCGCGAACAGTTTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATc < 1:1821258/70‑1 (MQ=255) gTTTCTTCAATGCGCGAACAGAGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCt > 1:2216941/1‑71 (MQ=255) gTTTCTTCAAAGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGtt > 1:3381466/1‑63 (MQ=255) tttCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAg < 1:2307460/60‑1 (MQ=255) tttCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAg < 1:2996854/60‑1 (MQ=255) tttCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAg < 1:520318/60‑1 (MQ=255) tttCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAg < 1:1511159/60‑1 (MQ=255) tttCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCtt < 1:966073/71‑1 (MQ=255) tttCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCtt < 1:3642418/71‑1 (MQ=255) ttcttcAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTg < 1:1641244/63‑1 (MQ=255) tcttcAATGCACGAACATTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCtt > 1:1655052/1‑69 (MQ=255) tcttcAATGCACGAACAGTGTCGATTTCTTGCTCGcc > 1:1543770/1‑37 (MQ=255) tcttcAATGCACGAACAGTGTCGATTTCTTGCTCGc > 1:1095238/1‑36 (MQ=255) tcttcAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCttt > 1:3467114/1‑70 (MQ=255) tcttcAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCttt < 1:488714/70‑1 (MQ=255) cttcAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTa < 1:184319/70‑1 (MQ=255) ttcAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCtt < 1:791550/67‑1 (MQ=255) cAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAg < 1:3658869/54‑1 (MQ=255) cAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTaa < 1:1760680/68‑1 (MQ=255) cAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTaa < 1:1536113/68‑1 (MQ=255) cAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAg < 1:3517427/70‑1 (MQ=255) aTGCACGAACAGTGTCGATTTCTTGCTCGCCAGGt < 1:2479639/35‑1 (MQ=255) aTGCACGAACAGTGTCGATTTCTTGCTCGCCAGGt < 1:20895/35‑1 (MQ=255) aTGCACGAACAGTGTCGATTTCTTGCTCGCCAGGt < 1:1653479/35‑1 (MQ=255) aTGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCg < 1:124940/71‑1 (MQ=255) tGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTaa < 1:3685257/36‑1 (MQ=255) tGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTaa < 1:3493471/36‑1 (MQ=255) cACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAg > 1:3708974/1‑71 (MQ=255) aCGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGc < 1:2231568/71‑1 (MQ=255) | TCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGC > minE/1889501‑1889639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |