| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,900,258 | G→A | 29.6% | A928V (GCT→GTT) | recB ← | exonuclease V (RecBCD complex), beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,900,258 | 0 | G | A | 29.6% | 101.4 / 51.6 | 71 | A928V (GCT→GTT) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (26/24); new base A (8/13); total (34/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGCT > minE/1900188‑1900328 | cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:361916/1‑71 (MQ=255) ggTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:1288992/1‑70 (MQ=255) tGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCAt > 1:1652640/1‑71 (MQ=255) aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc < 1:469138/70‑1 (MQ=255) cACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACa > 1:1345197/1‑71 (MQ=255) aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACAt > 1:2544009/1‑71 (MQ=255) aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACAt > 1:2922483/1‑71 (MQ=255) cgcgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATc > 1:3127706/1‑70 (MQ=255) cgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAg > 1:2956039/1‑71 (MQ=255) cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:3276163/71‑1 (MQ=255) cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:2318/71‑1 (MQ=255) cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:372064/71‑1 (MQ=255) cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGcc < 1:11998/71‑1 (MQ=255) ggAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCg < 1:2235723/71‑1 (MQ=255) gAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGa < 1:1169303/71‑1 (MQ=255) aaaCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAg > 1:2488333/1‑71 (MQ=255) aaaCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAg > 1:3314224/1‑71 (MQ=255) aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:976367/1‑70 (MQ=255) aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:2366136/1‑70 (MQ=255) aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:971639/1‑70 (MQ=255) aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:1462274/1‑70 (MQ=255) aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTAGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:3119208/1‑70 (MQ=255) cTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCa < 1:1685675/71‑1 (MQ=255) gATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCAt > 1:174729/1‑70 (MQ=255) aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATc > 1:3265652/1‑70 (MQ=255) aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATc > 1:2171646/1‑70 (MQ=255) aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGa > 1:1021553/1‑64 (MQ=255) tgtgGTGTTAACGTCGTTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGa < 1:2159110/63‑1 (MQ=255) tgtgGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGa < 1:594362/63‑1 (MQ=255) tggtgTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCaaa < 1:2691484/70‑1 (MQ=255) tggtgTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCaaa < 1:2265630/70‑1 (MQ=255) ggtgTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATc > 1:3908236/1‑71 (MQ=255) gtgtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATcc < 1:220629/71‑1 (MQ=255) tgtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt < 1:2146882/71‑1 (MQ=255) gtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGa > 1:203510/1‑57 (MQ=255) ttAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcagca < 1:2817580/41‑1 (MQ=255) ttAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTg < 1:3303861/70‑1 (MQ=255) tAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATc < 1:3097454/66‑1 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg > 1:2392536/1‑70 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg < 1:782903/70‑1 (MQ=255) gTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCt < 1:2418723/64‑1 (MQ=255) ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCg < 1:3121309/70‑1 (MQ=255) ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCg < 1:3101828/70‑1 (MQ=255) ttcttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCGAATCCTGGGCGATACCGt < 1:3556382/71‑1 (MQ=255) ttcttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:3279373/71‑1 (MQ=255) ttcttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:1715766/71‑1 (MQ=255) tcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt > 1:1637013/1‑70 (MQ=255) tcttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGc < 1:3498359/62‑1 (MQ=255) tcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAc > 1:1603009/1‑70 (MQ=255) cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGa < 1:2467533/41‑1 (MQ=255) cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAcc < 1:1350217/70‑1 (MQ=255) cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAc < 1:3843017/69‑1 (MQ=255) cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCa > 1:1409/1‑71 (MQ=255) cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCa > 1:2632049/1‑71 (MQ=255) cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCa > 1:2059225/1‑71 (MQ=255) cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCa > 1:1608748/1‑71 (MQ=255) cgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGc < 1:2105720/71‑1 (MQ=255) cgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGc < 1:3850063/71‑1 (MQ=255) cgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGc > 1:903526/1‑71 (MQ=255) acgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTg < 1:3023548/71‑1 (MQ=255) cgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGTCGATACCGTGACCACGCTg < 1:496617/70‑1 (MQ=255) cgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGt < 1:1421276/71‑1 (MQ=255) cTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt < 1:2025463/46‑1 (MQ=255) cTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt < 1:2623486/46‑1 (MQ=255) cTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTg > 1:1185949/1‑71 (MQ=255) cTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCaaa < 1:772811/42‑1 (MQ=255) gCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGtt > 1:42441/1‑67 (MQ=255) gCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCa < 1:1297523/70‑1 (MQ=255) aaCGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAAc > 1:1984538/1‑71 (MQ=255) aCGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAAc > 1:2516469/1‑70 (MQ=255) cGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCaatcg > 1:678450/1‑67 (MQ=255) gCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAAc > 1:1652488/1‑68 (MQ=255) cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCagag > 1:2957942/1‑71 (MQ=255) cTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGa < 1:699393/50‑1 (MQ=255) gcagcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTg > 1:1404971/1‑59 (MQ=255) gcaacaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAACACGCTGTTGCAAACCagag > 1:1168939/1‑69 (MQ=255) cagcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAAcc > 1:3456724/1‑64 (MQ=255) cagcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAg < 1:2115534/71‑1 (MQ=255) agcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGc > 1:1166110/1‑71 (MQ=255) gcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGCt < 1:1081733/71‑1 (MQ=255) | CGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGCT > minE/1900188‑1900328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |