| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,914,596 | T→G | 11.7% | K292N (AAA→AAC) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,914,596 | 0 | T | G | 11.7% | 130.0 / 14.8 | 60 | K292N (AAA→AAC) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base T (27/26); new base G (5/2); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
CCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCC > minE/1914527‑1914662 | cccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCtt < 1:2738593/71‑1 (MQ=255) ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt < 1:645265/71‑1 (MQ=255) ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt > 1:1949734/1‑71 (MQ=255) cTTGATTAGCGTCATCAAGATGAAACAGCAATCTCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt > 1:3011878/1‑70 (MQ=255) cTTGATGACGGTAATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt > 1:10264/1‑70 (MQ=255) tGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt > 1:1036195/1‑68 (MQ=255) gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt > 1:126892/1‑67 (MQ=255) aTTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTgaga < 1:610399/70‑1 (MQ=255) aGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGt > 1:3316572/1‑71 (MQ=255) aGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGt > 1:2987585/1‑71 (MQ=255) gCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGt > 1:519013/1‑70 (MQ=255) cGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTa > 1:2670947/1‑71 (MQ=255) catcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCa > 1:1399041/1‑71 (MQ=255) atcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCa < 1:2023173/70‑1 (MQ=255) tcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTga < 1:1342895/58‑1 (MQ=255) aaGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTAt > 1:2948455/1‑65 (MQ=255) aaGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACtt < 1:710778/70‑1 (MQ=255) aaGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACt > 1:3510027/1‑69 (MQ=255) aGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAg > 1:3441845/1‑71 (MQ=255) tGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgc > 1:2692536/1‑69 (MQ=255) aaCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCgg > 1:919427/1‑70 (MQ=255) aaCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCgg > 1:1951227/1‑70 (MQ=255) aCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGc > 1:1666671/1‑70 (MQ=255) aCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGc > 1:2627759/1‑70 (MQ=255) aCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCa < 1:2194515/71‑1 (MQ=255) aCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCa < 1:3032739/71‑1 (MQ=255) cAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGAt > 1:3233661/1‑47 (MQ=255) cAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTAt > 1:822162/1‑53 (MQ=255) cAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACt > 1:1225366/1‑71 (MQ=255) aaTCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTg < 1:3291589/71‑1 (MQ=255) aaTCGCTGACCCAGCGCACGTAAATCGCCAGCCAGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTg > 1:342685/1‑71 (MQ=255) aTCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTgaga < 1:455698/44‑1 (MQ=255) aTCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATa < 1:136011/46‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGtt < 1:665325/71‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGtt < 1:1336536/71‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGtt < 1:336762/71‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGtt < 1:2672754/71‑1 (MQ=255) cGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTc > 1:3059825/1‑71 (MQ=255) cGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTc > 1:3867702/1‑71 (MQ=255) cGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTc > 1:328773/1‑71 (MQ=255) gCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCg < 1:2473509/71‑1 (MQ=255) gCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCg > 1:2289466/1‑71 (MQ=255) cTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCAc > 1:625755/1‑50 (MQ=255) tGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCTCGGCAAACTGTTCgg > 1:1670026/1‑70 (MQ=255) tGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCgga > 1:1130362/1‑70 (MQ=255) gACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGc > 1:1620572/1‑70 (MQ=255) aCCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCa < 1:243539/70‑1 (MQ=255) aGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCa < 1:1549206/42‑1 (MQ=255) cgcACGTAAATCGCCAGCCCGTTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAttttt < 1:597973/71‑1 (MQ=255) cgcACGTAAATCGCCAGCCCGCTCTTTGAGATATTTAt < 1:1910067/38‑1 (MQ=255) aCGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAAc > 1:1795266/1‑52 (MQ=255) gTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACtt > 1:3381337/1‑38 (MQ=255) tAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgcg > 1:1696927/1‑41 (MQ=255) tAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCa < 1:3839441/58‑1 (MQ=255) tAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAttttt < 1:394930/65‑1 (MQ=255) tCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGt < 1:1566837/71‑1 (MQ=255) gCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCa < 1:3405037/52‑1 (MQ=255) gCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCa < 1:1001236/52‑1 (MQ=255) gCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGttt > 1:3200049/1‑71 (MQ=255) aGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTa < 1:970479/71‑1 (MQ=255) gCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAtttt < 1:3188807/54‑1 (MQ=255) gCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAtttt < 1:3192888/54‑1 (MQ=255) gCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAtttt > 1:2879989/1‑54 (MQ=255) ccGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATg < 1:3679620/58‑1 (MQ=255) cGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCatat < 1:358518/48‑1 (MQ=255) gCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGcc > 1:1456552/1‑71 (MQ=255) gCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGcc > 1:2117551/1‑71 (MQ=255) tctTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAttttt < 1:294600/49‑1 (MQ=255) | CCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCC > minE/1914527‑1914662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |