| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,932,763 | G→A | 65.4% | intergenic (‑134/+162) | yqeF ← / ← lysS | predicted acyltransferase/lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,932,763 | 0 | G | A | 65.4% | 82.2 / 73.4 | 81 | intergenic (‑134/+162) | yqeF/lysS | predicted acyltransferase/lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base A (26/27); total (41/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTT > minE/1932697‑1932831 | aaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGt < 1:2020001/70‑1 (MQ=255)aaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGt < 1:2929275/70‑1 (MQ=255) aaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGttt > 1:3686116/1‑71 (MQ=255) aaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGttt > 1:3302701/1‑71 (MQ=255) aaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTg > 1:227862/1‑71 (MQ=255) aaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTg > 1:1820570/1‑70 (MQ=255) aaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGc < 1:6872/71‑1 (MQ=255) aaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGc < 1:3332355/71‑1 (MQ=255) aGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCaa < 1:3697110/71‑1 (MQ=255) aGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCaa > 1:2671831/1‑71 (MQ=255) gTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAAc < 1:238748/71‑1 (MQ=255) tGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAAcc < 1:396964/71‑1 (MQ=255) tttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACt < 1:1238681/71‑1 (MQ=255) tttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACt < 1:3594304/71‑1 (MQ=255) ttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTaa < 1:2443861/70‑1 (MQ=255) ttATCACAAAAGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAAc > 1:703995/1‑71 (MQ=255) aTCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAAcc < 1:3298916/70‑1 (MQ=255) caAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACt < 1:444331/62‑1 (MQ=255) caAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACccac < 1:711887/69‑1 (MQ=255) aaaGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGttt > 1:1901056/1‑52 (MQ=255) aaGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAAc > 1:3091731/1‑56 (MQ=255) aaGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTaa > 1:3836110/1‑62 (MQ=255) aGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGt < 1:831622/48‑1 (MQ=255) aGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCaa > 1:1328981/1‑69 (MQ=255) aGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCaa > 1:1069696/1‑69 (MQ=255) aGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACcca > 1:2115585/1‑65 (MQ=255) aGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACcca > 1:914075/1‑65 (MQ=255) ggAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa < 1:3314606/70‑1 (MQ=255) gAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCa > 1:271425/1‑55 (MQ=255) gAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATaga < 1:1270837/71‑1 (MQ=255) aaaTATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATagag > 1:199428/1‑71 (MQ=255) tatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCa < 1:1247822/51‑1 (MQ=255) cTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa > 1:548645/1‑70 (MQ=255) cTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGAt < 1:1121292/71‑1 (MQ=255) tGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa > 1:1055590/1‑69 (MQ=255) tGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa > 1:2432192/1‑69 (MQ=255) gAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAg > 1:2123775/1‑71 (MQ=255) gAGCAGCAGTCAGAGACATAACTGGCACGTAAGGt > 1:1135732/1‑35 (MQ=255) gAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTaa > 1:3210257/1‑48 (MQ=255) agcagcAGTCAGAGACATAACTGGCACGTAGGGTTTGCAAc > 1:3044318/1‑41 (MQ=255) agcagcAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACcca > 1:2342821/1‑51 (MQ=255) agcagcAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAgg > 1:2943157/1‑71 (MQ=255) agcagcAGTCAGAGACATAAATGGCACGTAAGGTTTGCAACCACTAACcca > 1:1907028/1‑51 (MQ=255) gcagcaGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAg > 1:2211150/1‑69 (MQ=255) cagcagTCAGAGACATAACTGGCACGTAGGGTTTGc < 1:205775/36‑1 (MQ=255) gcagTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGt < 1:3346316/71‑1 (MQ=255) cagTCAGAGACATAACTGGCACGTAAGGTTTGCaa < 1:3869854/35‑1 (MQ=255) agTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAgggg < 1:870562/57‑1 (MQ=255) agTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:2413032/71‑1 (MQ=255) tCAGAGACATAACTGGCACGTAGGGTTTGCAACCACt < 1:2938119/37‑1 (MQ=255) tCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATag < 1:1995093/50‑1 (MQ=255) tCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGtt < 1:981639/68‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTa < 1:140714/57‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTa < 1:447114/57‑1 (MQ=255) cAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGAt > 1:2834728/1‑59 (MQ=255) cAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGAt > 1:3798679/1‑59 (MQ=255) cAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAtc > 1:2829020/1‑71 (MQ=255) agagACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAtct > 1:2451562/1‑71 (MQ=255) agagACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAtc < 1:803121/70‑1 (MQ=255) agagACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAggg < 1:490953/52‑1 (MQ=255) agagACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAtct < 1:207220/71‑1 (MQ=255) cATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCAt < 1:3105313/71‑1 (MQ=255) tAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAtctc < 1:2601437/65‑1 (MQ=255) tAACTGGCACGTAATGTTTGCAACCACTAACCCACCAATagag < 1:2181103/43‑1 (MQ=255) tAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAggg < 1:719858/45‑1 (MQ=255) aaCTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCg > 1:2804534/1‑56 (MQ=255) aaCTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATAc > 1:217817/1‑70 (MQ=255) aCTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACt > 1:290831/1‑70 (MQ=255) cTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACtt > 1:2966368/1‑70 (MQ=255) gCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAcc > 1:1045653/1‑71 (MQ=255) gCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAcc < 1:251644/71‑1 (MQ=255) gCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAcc < 1:3245856/71‑1 (MQ=255) gCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAcc > 1:1536392/1‑71 (MQ=255) gCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAcc < 1:2216762/71‑1 (MQ=255) gCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAcc > 1:3906007/1‑71 (MQ=255) cACGTAAGGTTTGCAACCACTAACCCACCAATaga > 1:737593/1‑35 (MQ=255) cACGTAAGGTTTGCAACCACTAACCCACCAATAGAgggg < 1:1068254/39‑1 (MQ=255) gTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGt < 1:2863169/71‑1 (MQ=255) gTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGt > 1:3380421/1‑71 (MQ=255) tAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATa > 1:2694740/1‑41 (MQ=255) tAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCCCCCATACTTAACCTGGtt > 1:2417842/1‑71 (MQ=255) | AAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTT > minE/1932697‑1932831 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |