Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,932,853 C→T 27.6% intergenic (‑224/+72) yqeF ← / ← lysS predicted acyltransferase/lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,932,8530CT27.6% 41.4 / 16.4 29intergenic (‑224/+72)yqeF/lysSpredicted acyltransferase/lysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (13/8);  new base T (4/4);  total (17/12)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

ACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCGACATTTGCCGGGGTTGTGAGC  >  minE/1932783‑1932912
                                                                      |                                                           
accaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTc                                                             >  1:3648297/1‑71 (MQ=255)
     tAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGttt                                                        <  1:2683253/71‑1 (MQ=255)
            gTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTg                                                  >  1:3490850/1‑70 (MQ=255)
                aTAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAgat                                             <  1:565640/71‑1 (MQ=255)
                aTAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGt                                                          <  1:502145/58‑1 (MQ=255)
                aTAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTg                                                  <  1:3346974/66‑1 (MQ=255)
                   gggCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAgatgat                                          <  1:307319/71‑1 (MQ=255)
                    ggCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATa                                         >  1:3446160/1‑71 (MQ=255)
                    ggCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAAt                                                     <  1:489305/59‑1 (MQ=255)
                       gTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGt                                      >  1:574325/1‑71 (MQ=255)
                         tAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGtt                                     >  1:898307/1‑70 (MQ=255)
                         tAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTc                                    >  1:1445673/1‑71 (MQ=255)
                              tcCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCTaaa                                >  1:812249/1‑70 (MQ=255)
                                ccATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTcaga                                 >  1:3639884/1‑64 (MQ=255)
                                ccATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAAtt                                                    <  1:3219859/48‑1 (MQ=255)
                                ccATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTATATGATACGTTCTaa                                 <  1:2781125/67‑1 (MQ=255)
                                  aTACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAgat                                             >  1:925625/1‑53 (MQ=255)
                                   tACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTg                                                  <  1:3930183/47‑1 (MQ=255)
                                        aaCCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCg                       >  1:3715655/1‑69 (MQ=255)
                                         aCCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATAc                                        >  1:704613/1‑51 (MQ=255)
                                         aCCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCGAc                     <  1:1615977/70‑1 (MQ=255)
                                          ccTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGt                                                 >  1:2810597/1‑41 (MQ=255)
                                              gTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCTaaaaa                              >  1:2003075/1‑56 (MQ=255)
                                                 tATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCGACATTTGCCgg            >  1:3631784/1‑71 (MQ=255)
                                                  aTGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGt                                      <  1:2336583/44‑1 (MQ=255)
                                                        aaTTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCGACATTTGCCTGGGTtgtg     <  1:381171/71‑1 (MQ=255)
                                                        aaTTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCGACATTTGCCGGGGTtgt      >  1:3249160/1‑70 (MQ=255)
                                                               cTCCATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCGACATTTGCCGGGGTtg       >  1:3931901/1‑62 (MQ=255)
                                                                 ccATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCGACATTTGCCGGGGTTGTGAg   >  1:2259759/1‑64 (MQ=255)
                                                                 ccATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCGACATTTGCCGGGGTTGTGAGc  >  1:174608/1‑65 (MQ=255)
                                                                      |                                                           
ACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCTAAAAAAACCCCGACATTTGCCGGGGTTGTGAGC  >  minE/1932783‑1932912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: