| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,932,952 | C→G | 10.0% | L497L (CTG→CTC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,932,952 | 0 | C | G | 10.0% | 226.9 / 12.8 | 80 | L497L (CTG→CTC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (42/30); new base G (2/6); total (44/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGA > minE/1932883‑1933021 | aaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACa < 1:3511213/71‑1 (MQ=255) aaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAg > 1:1444503/1‑70 (MQ=255) aCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGaa > 1:3369038/1‑71 (MQ=255) aCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGa > 1:3018266/1‑70 (MQ=255) cccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATa < 1:3796069/71‑1 (MQ=255) ccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATaa > 1:1444569/1‑71 (MQ=255) gACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACg > 1:3567785/1‑71 (MQ=255) cATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTc > 1:2945439/1‑71 (MQ=255) aTTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACa < 1:173788/60‑1 (MQ=255) ttGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgcg > 1:3018044/1‑71 (MQ=255) gCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACa < 1:3562910/56‑1 (MQ=255) cGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:2432956/1‑71 (MQ=255) cGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:1225247/1‑71 (MQ=255) ggggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa < 1:287509/68‑1 (MQ=255) ggggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa < 1:2862970/68‑1 (MQ=255) gggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGt < 1:41688/71‑1 (MQ=255) ggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:3629386/1‑71 (MQ=255) ggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:1318654/1‑71 (MQ=255) gTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt < 1:2428158/71‑1 (MQ=255) tgAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGc < 1:540989/70‑1 (MQ=255) gAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTg > 1:2583037/1‑71 (MQ=255) gCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAAt < 1:1629071/47‑1 (MQ=255) gCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg > 1:1979411/1‑66 (MQ=255) gCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg > 1:950560/1‑66 (MQ=255) tAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcg < 1:155957/51‑1 (MQ=255) tAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcg < 1:286593/51‑1 (MQ=255) aaCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt > 1:2621741/1‑70 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGaa > 1:1927194/1‑41 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGaa > 1:589158/1‑41 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg < 1:2531095/70‑1 (MQ=255) cGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGaa < 1:2635929/71‑1 (MQ=255) tAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGt > 1:3772398/1‑62 (MQ=255) aaTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATg > 1:653092/1‑56 (MQ=255) aaTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:645167/1‑71 (MQ=255) aTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:3127157/1‑70 (MQ=255) aTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:3537682/1‑70 (MQ=255) ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:1149017/1‑71 (MQ=255) tATTTTACCGGACGCATCGCCGGGAACAGAATAACGt < 1:1271524/37‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:2293919/54‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAcc < 1:1169249/70‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:878366/1‑71 (MQ=255) ttttACCGGACGCATCGCCGCGAACAGCATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:2314682/1‑71 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt < 1:2323056/45‑1 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt < 1:2990411/45‑1 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt > 1:3650037/1‑64 (MQ=255) tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa < 1:1280495/70‑1 (MQ=255) tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATAc > 1:2324401/1‑71 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCGGTTGGTGAACAGCATTACCATACg < 1:3049851/70‑1 (MQ=255) ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt > 1:1446317/1‑47 (MQ=255) ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc < 1:1995375/71‑1 (MQ=255) ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc < 1:142934/71‑1 (MQ=255) ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc < 1:3554281/71‑1 (MQ=255) gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc > 1:2499762/1‑70 (MQ=255) gCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt > 1:3378994/1‑55 (MQ=255) gCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt > 1:1704730/1‑55 (MQ=255) cATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg < 1:255271/43‑1 (MQ=255) cATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg < 1:2212840/43‑1 (MQ=255) cATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg < 1:2511229/43‑1 (MQ=255) aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGt > 1:3600651/1‑40 (MQ=255) aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:342351/1‑58 (MQ=255) aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAc > 1:399179/1‑70 (MQ=255) aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAc > 1:1589153/1‑70 (MQ=255) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:998539/40‑1 (MQ=255) cGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg > 1:1545448/1‑41 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaaa > 1:2507471/1‑44 (MQ=21) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:389074/1‑70 (MQ=255) ccGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATa < 1:2249842/65‑1 (MQ=255) ccGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAAta < 1:1387639/71‑2 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt > 1:2309855/1‑70 (MQ=255) cGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAAta < 1:3545116/70‑2 (MQ=255) gggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAcc < 1:2036599/71‑1 (MQ=255) ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAt < 1:2573551/52‑1 (MQ=255) ggAACAGAATAACGTCGCGGATGGTATGGCTGTTTGTGat < 1:113343/40‑2 (MQ=39) ggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa < 1:957812/60‑1 (MQ=255) ggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:2420740/1‑70 (MQ=255) gAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCa < 1:3836490/71‑1 (MQ=255) gAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:51047/1‑69 (MQ=255) gAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:446491/1‑69 (MQ=255) aaCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:2017540/1‑68 (MQ=255) aCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGa > 1:2874504/1‑71 (MQ=255) | AAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGA > minE/1932883‑1933021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |