| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,932,967 | G→A | 11.9% | I492I (ATC→ATT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,932,967 | 0 | G | A | 11.9% | 228.7 / 17.3 | 84 | I492I (ATC→ATT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (43/31); new base A (4/6); total (47/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01 | |||||||||||
CGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGG > minE/1932900‑1933035 | cGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:1225247/1‑71 (MQ=255)cGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:2432956/1‑71 (MQ=255) ggggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa < 1:2862970/68‑1 (MQ=255) ggggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa < 1:287509/68‑1 (MQ=255) gggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGt < 1:41688/71‑1 (MQ=255) ggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:3629386/1‑71 (MQ=255) ggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:1318654/1‑71 (MQ=255) gTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt < 1:2428158/71‑1 (MQ=255) tgAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGc < 1:540989/70‑1 (MQ=255) gAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTg > 1:2583037/1‑71 (MQ=255) gCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg > 1:950560/1‑66 (MQ=255) gCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg > 1:1979411/1‑66 (MQ=255) aaCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt > 1:2621741/1‑70 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg < 1:2531095/70‑1 (MQ=255) cGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGaa < 1:2635929/71‑1 (MQ=255) tAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGt > 1:3772398/1‑62 (MQ=255) aaTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATg > 1:653092/1‑56 (MQ=255) aaTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:645167/1‑71 (MQ=255) aTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:3127157/1‑70 (MQ=255) aTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:3537682/1‑70 (MQ=255) ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:1149017/1‑71 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:2293919/54‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAcc < 1:1169249/70‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:878366/1‑71 (MQ=255) ttttACCGGACGCATCGCCGCGAACAGCATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:2314682/1‑71 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt < 1:2990411/45‑1 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt < 1:2323056/45‑1 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt > 1:3650037/1‑64 (MQ=255) tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa < 1:1280495/70‑1 (MQ=255) tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATAc > 1:2324401/1‑71 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCGGTTGGTGAACAGCATTACCATACg < 1:3049851/70‑1 (MQ=255) ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt > 1:1446317/1‑47 (MQ=255) ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc < 1:142934/71‑1 (MQ=255) ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc < 1:1995375/71‑1 (MQ=255) ggACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc < 1:3554281/71‑1 (MQ=255) gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc > 1:2499762/1‑70 (MQ=255) gCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt > 1:1704730/1‑55 (MQ=255) gCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt > 1:3378994/1‑55 (MQ=255) cATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg < 1:2511229/43‑1 (MQ=255) cATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg < 1:255271/43‑1 (MQ=255) cATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg < 1:2212840/43‑1 (MQ=255) aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGt > 1:3600651/1‑40 (MQ=255) aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:342351/1‑58 (MQ=255) aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAc > 1:1589153/1‑70 (MQ=255) aTCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAc > 1:399179/1‑70 (MQ=255) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:998539/40‑1 (MQ=255) cGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg > 1:1545448/1‑41 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaaa > 1:2507471/1‑44 (MQ=21) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:389074/1‑70 (MQ=255) ccGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATa < 1:2249842/65‑1 (MQ=255) ccGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAAta < 1:1387639/71‑2 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt > 1:2309855/1‑70 (MQ=255) cGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAAta < 1:3545116/70‑2 (MQ=255) gggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAcc < 1:2036599/71‑1 (MQ=255) ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAt < 1:2573551/52‑1 (MQ=255) ggAACAGAATAACGTCGCGGATGGTATGGCTGTTTGTGat < 1:113343/40‑2 (MQ=39) ggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa < 1:957812/60‑1 (MQ=255) ggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:2420740/1‑70 (MQ=255) gAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCa < 1:3836490/71‑1 (MQ=255) gAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:51047/1‑69 (MQ=255) gAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:446491/1‑69 (MQ=255) aaCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:2017540/1‑68 (MQ=255) aCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGa > 1:2874504/1‑71 (MQ=255) gaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc > 1:2195732/2‑71 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt < 1:3511196/42‑1 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:1159958/1‑41 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa > 1:2444327/1‑46 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa > 1:2073444/1‑46 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg > 1:729160/1‑71 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg < 1:2702998/71‑1 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg > 1:3221528/1‑71 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGAATTACCATa > 1:2595537/1‑46 (MQ=255) aaTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAcca > 1:439714/1‑69 (MQ=255) aTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgc > 1:265374/1‑71 (MQ=255) tAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:2236446/71‑1 (MQ=255) aaCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgctg > 1:881339/1‑71 (MQ=255) aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt < 1:1883498/71‑1 (MQ=255) aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt < 1:2107835/71‑1 (MQ=255) gTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc > 1:3812308/1‑45 (MQ=255) gTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:1849638/67‑1 (MQ=255) tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc < 1:1516799/61‑1 (MQ=255) tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:2579557/66‑1 (MQ=255) tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcg > 1:3709333/1‑70 (MQ=255) cgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg < 1:949646/71‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:596495/62‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg > 1:891360/1‑70 (MQ=255) | CGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGG > minE/1932900‑1933035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |