Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 309,855 A→C 11.4% G1006G (GGT→GGG acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE309,8550AC11.4% 144.0 / 15.9 70G1006G (GGT→GGGacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (36/26);  new base C (4/4);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

GAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGA  >  minE/309785‑309925
                                                                      |                                                                      
gAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTaca                                                                        <  1:324198/71‑1 (MQ=255)
   ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCg                                                                     >  1:834336/1‑71 (MQ=255)
       cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc                                                                 >  1:968756/1‑71 (MQ=255)
        acaAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAcc                                                                >  1:3566960/1‑71 (MQ=255)
          aaaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                                              >  1:1611431/1‑71 (MQ=255)
            aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                                              >  1:185324/1‑67 (MQ=255)
             gAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTg                                                           >  1:1474655/1‑71 (MQ=255)
               acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                                                         <  1:3082222/69‑1 (MQ=255)
               aTACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCg                                                         <  1:350623/71‑1 (MQ=255)
                 aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                                       <  1:1227198/71‑1 (MQ=255)
                  ccGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTc                                                      <  1:5099/71‑1 (MQ=255)
                  ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGtt                                                       <  1:912952/70‑1 (MQ=255)
                   cGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCt                                                     <  1:756989/71‑1 (MQ=255)
                     gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc                                                   >  1:3107272/1‑71 (MQ=255)
                      taCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcg                                                  >  1:350509/2‑71 (MQ=255)
                       aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                                                 >  1:1502455/1‑71 (MQ=255)
                       aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                                                 <  1:3761026/71‑1 (MQ=255)
                       aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                                                 <  1:3699715/71‑1 (MQ=255)
                       aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcg                                                  >  1:2198179/1‑70 (MQ=255)
                       aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGGTCTgcgc                                                 <  1:1468783/71‑1 (MQ=255)
                        cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg                                                <  1:2391592/71‑1 (MQ=255)
                         gacgaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc                                               <  1:3693684/68‑1 (MQ=255)
                         gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc                                               <  1:844117/71‑1 (MQ=255)
                         gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc                                               >  1:2518292/1‑71 (MQ=255)
                            gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                                            <  1:1516309/71‑1 (MQ=255)
                            gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCg                                             >  1:432992/1‑70 (MQ=255)
                             aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                                            >  1:343039/1‑70 (MQ=255)
                             aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                                            >  1:1533154/1‑70 (MQ=255)
                               gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                                         <  1:1058779/71‑1 (MQ=255)
                                 ttGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCa                                       <  1:1277295/71‑1 (MQ=255)
                                  tGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg                                      >  1:2240384/1‑71 (MQ=255)
                                       gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc                                   >  1:3017790/1‑69 (MQ=255)
                                       gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc                                   >  1:2411339/1‑69 (MQ=255)
                                        tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGcc                                              >  1:2374236/1‑57 (MQ=255)
                                        tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGcc                                              >  1:3569415/1‑57 (MQ=255)
                                         aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCa                                 >  1:467581/1‑69 (MQ=255)
                                         aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg                                <  1:447574/70‑1 (MQ=255)
                                           cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTc                                                      <  1:2388672/46‑1 (MQ=255)
                                           cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTAc                             <  1:3538927/71‑1 (MQ=255)
                                           cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTAc                             >  1:291112/1‑71 (MQ=255)
                                           cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTAc                             >  1:1971779/1‑71 (MQ=255)
                                             ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc                                   <  1:1483380/63‑1 (MQ=255)
                                               gCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGa                          >  1:2202386/1‑70 (MQ=255)
                                                cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt                         <  1:1876620/70‑1 (MQ=255)
                                                 ggTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                                                    <  1:2328175/42‑1 (MQ=255)
                                                 ggTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                                                    <  1:1274505/42‑1 (MQ=255)
                                                   tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCt                                                     <  1:3047510/39‑1 (MQ=255)
                                                   tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCt                                                     <  1:1327094/39‑1 (MQ=255)
                                                   tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc                      >  1:328478/1‑70 (MQ=255)
                                                   tCACCATCCCCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAcc                                  <  1:155341/57‑1 (MQ=255)
                                                    cACCATCCCGCCCATTACACCGGTACCTACTGCGTTc                                                      >  1:1549396/1‑37 (MQ=255)
                                                    cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg                                >  1:2778931/1‑59 (MQ=255)
                                                    cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc                      >  1:2954398/1‑69 (MQ=255)
                                                    cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc                      >  1:3761830/1‑69 (MQ=255)
                                                     aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACt                            <  1:3247268/62‑1 (MQ=255)
                                                     aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCa                    >  1:927027/1‑70 (MQ=255)
                                                     aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCa                    <  1:3657933/70‑1 (MQ=255)
                                                     aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg                   >  1:1082288/1‑71 (MQ=255)
                                                     aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg                   >  1:18761/1‑71 (MQ=255)
                                                       cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCTGAACCAGCACCAGTACTGATAACCAGCg                 <  1:3407678/71‑1 (MQ=255)
                                                       cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg                 >  1:3805356/1‑71 (MQ=255)
                                                       cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg                 >  1:1425086/1‑71 (MQ=255)
                                                         tCCCGCCCATTACACCGGTACCTACTGCGTTCTgcg                                                  >  1:2616681/1‑36 (MQ=255)
                                                         tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCgg                >  1:2289047/1‑70 (MQ=255)
                                                          cccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa              >  1:1801455/1‑71 (MQ=255)
                                                           ccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg                 <  1:409043/67‑1 (MQ=255)
                                                              cccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATaa           >  1:1930723/1‑70 (MQ=255)
                                                              cccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAAc          >  1:2299343/1‑71 (MQ=255)
                                                              cccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAAc          >  1:3348179/1‑71 (MQ=255)
                                                               ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt                         <  1:1395343/55‑1 (MQ=255)
                                                                 aTTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc       >  1:1702184/1‑71 (MQ=255)
                                                                    acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc                                   >  1:3937843/1‑40 (MQ=255)
                                                                    acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTa                              >  1:1573387/1‑45 (MQ=255)
                                                                    acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGa     <  1:607219/70‑1 (MQ=255)
                                                                      acCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa  <  1:771483/71‑1 (MQ=255)
                                                                      acCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa  <  1:3418941/71‑1 (MQ=255)
                                                                      |                                                                      
GAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGA  >  minE/309785‑309925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: