Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,024 T→A 13.6% A473A (GCA→GCT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,0240TA13.6% 286.5 / 34.1 110A473A (GCA→GCTlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (49/46);  new base A (9/6);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 129 total aligned reads displayed.

AATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTC  >  minE/1932955‑1933092
                                                                     |                                                                    
aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                                                                     >  1:3221528/1‑71 (MQ=255)
aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                                                                     <  1:2702998/71‑1 (MQ=255)
aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                                                                     >  1:729160/1‑71 (MQ=255)
 aTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgc                                                                    >  1:265374/1‑71 (MQ=255)
  tAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct                                                                   <  1:2236446/71‑1 (MQ=255)
   aaCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgctg                                                                  >  1:881339/1‑71 (MQ=255)
    aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt                                                                 <  1:2107835/71‑1 (MQ=255)
    aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt                                                                 <  1:1883498/71‑1 (MQ=255)
       tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct                                                                   <  1:2579557/66‑1 (MQ=255)
       tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcg                                                               >  1:3709333/1‑70 (MQ=255)
          cgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg                                                           <  1:949646/71‑1 (MQ=255)
           ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg                                                           >  1:891360/1‑70 (MQ=255)
             atagTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTtggcgg                                                           >  1:3952323/4‑68 (MQ=255)
             aTGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt                                                          >  1:622335/1‑69 (MQ=255)
              tGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAg                                                       <  1:3250687/71‑1 (MQ=255)
               ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                                                      >  1:1002513/1‑71 (MQ=255)
               ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                                                      >  1:2167885/1‑71 (MQ=255)
               ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                                                      >  1:143374/1‑71 (MQ=255)
                  aTGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg                                                              <  1:1840271/60‑1 (MQ=255)
                   tGGCTGTTGGTGAACAGCATTATCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg                                                  >  1:1916970/1‑71 (MQ=255)
                   tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg                                                              >  1:2915610/1‑59 (MQ=255)
                   tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg                                                  >  1:205013/1‑71 (MQ=255)
                   tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCACAGACCTGCTGTCGGCGGTAAGCCATg                                                  >  1:1699821/1‑71 (MQ=255)
                    ggCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct                                                                   <  1:2707742/53‑1 (MQ=255)
                    ggCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa                                                    <  1:1754461/68‑1 (MQ=255)
                     gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGcc                                                     >  1:1147443/1‑66 (MQ=255)
                     gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt                                                 <  1:1736708/70‑1 (MQ=255)
                      cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc                                               <  1:1806520/71‑1 (MQ=255)
                      cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc                                               <  1:1157779/71‑1 (MQ=255)
                       tGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc                                               >  1:608211/1‑70 (MQ=255)
                         ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa                                             <  1:3740287/70‑1 (MQ=255)
                          tGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgctg                                                                  <  1:3630332/48‑1 (MQ=255)
                           ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGt                                           <  1:610347/70‑1 (MQ=255)
                            gTGAACAGCATTACCATACTGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg                                          <  1:81702/70‑1 (MQ=255)
                            gTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg                                          <  1:1431549/70‑1 (MQ=255)
                               aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag                                       <  1:1518668/70‑3 (MQ=255)
                               aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag                                       <  1:3533722/70‑3 (MQ=255)
                                aCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTc                                                                <  1:86598/44‑1 (MQ=255)
                                 cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa                                                    >  1:2858227/1‑55 (MQ=255)
                                  aGCATTACCATACGGTCGATACCAATTCCCAGACCTTCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa                                    <  1:950687/70‑1 (MQ=255)
                                   gCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACg                                  >  1:1010793/1‑71 (MQ=255)
                                    cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACg                                  >  1:448310/1‑70 (MQ=255)
                                    cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGt                                 <  1:2788785/71‑1 (MQ=255)
                                      ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc                                              <  1:2087148/56‑1 (MQ=255)
                                      ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa                               >  1:198306/1‑71 (MQ=255)
                                      ttACCATACGGTCGATACCAATTCCCAGACCTGCTCTCGGCGGTAAGCCATg                                                  <  1:2845356/52‑1 (MQ=255)
                                       tACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg                                                            <  1:164329/41‑1 (MQ=255)
                                       tACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAAt                              >  1:3450901/1‑71 (MQ=255)
                                        aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt                                                 >  1:306058/1‑51 (MQ=255)
                                        aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa                                             >  1:3916373/1‑55 (MQ=255)
                                        aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAAt                              <  1:3606931/70‑1 (MQ=255)
                                        aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc                             >  1:427271/1‑71 (MQ=255)
                                        aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc                             >  1:2344956/1‑71 (MQ=255)
                                           attcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTc                          <  1:1718938/68‑1 (MQ=255)
                                            tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa                         <  1:221560/71‑1 (MQ=255)
                                              cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTg                                     <  1:1049062/57‑1 (MQ=255)
                                              cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt                        <  1:2233645/70‑1 (MQ=255)
                                              cGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTcgt                        <  1:353542/70‑3 (MQ=255)
                                              cGGTAGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc                          >  1:935135/1‑68 (MQ=255)
                                               ggTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg                      <  1:2583459/71‑1 (MQ=255)
                                               ggTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg                      <  1:3665241/71‑1 (MQ=255)
                                                 tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCgg                                       <  1:1605072/52‑1 (MQ=255)
                                                 tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg                      >  1:227480/1‑69 (MQ=255)
                                                   gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTg                                     >  1:3552068/1‑52 (MQ=255)
                                                   gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg                      <  1:3441203/67‑1 (MQ=255)
                                                   gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                   >  1:1807213/1‑70 (MQ=255)
                                                   gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                   >  1:508248/1‑70 (MQ=255)
                                                   gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                   >  1:1471501/1‑70 (MQ=255)
                                                    aTACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa                 <  1:458509/71‑1 (MQ=255)
                                                    aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa                 <  1:549750/71‑1 (MQ=255)
                                                    aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa                 <  1:132951/71‑1 (MQ=255)
                                                     tACAAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa                  >  1:3182211/1‑69 (MQ=255)
                                                      aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt                                                 >  1:57054/1‑37 (MQ=255)
                                                      aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt                                                 >  1:829145/1‑37 (MQ=255)
                                                      aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAAc                >  1:2634888/1‑70 (MQ=255)
                                                      aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa               >  1:3843692/1‑71 (MQ=255)
                                                       ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa                                    >  1:1356223/1‑49 (MQ=255)
                                                       ccAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACAt              >  1:3047784/1‑71 (MQ=255)
                                                          aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc           >  1:2041472/1‑71 (MQ=255)
                                                          aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc           >  1:14951/1‑71 (MQ=255)
                                                          aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc           >  1:548675/1‑71 (MQ=255)
                                                           ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt          >  1:2388482/1‑71 (MQ=255)
                                                           ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt          <  1:1395080/71‑1 (MQ=255)
                                                             cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt                           <  1:405118/52‑1 (MQ=255)
                                                              ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg       <  1:1022371/71‑1 (MQ=255)
                                                              ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg       <  1:3455080/71‑1 (MQ=255)
                                                                aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt                        >  1:23360/1‑52 (MQ=255)
                                                                aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc             >  1:224271/1‑63 (MQ=255)
                                                                aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc     <  1:2562153/71‑1 (MQ=255)
                                                                aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc     >  1:1968959/1‑71 (MQ=255)
                                                                aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc     >  1:606744/1‑71 (MQ=255)
                                                                aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc     >  1:1605980/1‑71 (MQ=255)
                                                                 gACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtcg    >  1:3809992/1‑71 (MQ=255)
                                                                    ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt                     >  1:3359588/1‑51 (MQ=255)
                                                                    ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                   <  1:1392547/53‑1 (MQ=255)
                                                                     tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt                     >  1:1142805/1‑50 (MQ=255)
                                                                     tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt                     >  1:3108360/1‑50 (MQ=255)
                                                                     tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATTGCTtcgtc     >  1:1449694/1‑66 (MQ=255)
                                                                     tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc     >  1:1406876/1‑66 (MQ=255)
                                                                     tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc     >  1:491385/1‑66 (MQ=255)
                                                                     |                                                                    
AATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTC  >  minE/1932955‑1933092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: