| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,027 | T→G | 13.6% | T472T (ACA→ACC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,027 | 0 | T | G | 13.6% | 226.8 / 38.8 | 110 | T472T (ACA→ACC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (49/46); new base G (9/6); total (58/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
TAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTG > minE/1932957‑1933097 | tAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:2236446/71‑1 (MQ=255) aaCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgctg > 1:881339/1‑71 (MQ=255) aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt < 1:2107835/71‑1 (MQ=255) gTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:1849638/67‑1 (MQ=255) tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcg > 1:3709333/1‑70 (MQ=255) cgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg < 1:949646/71‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:596495/62‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg > 1:891360/1‑70 (MQ=255) atagTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTtggcgg > 1:3952323/4‑68 (MQ=255) aTGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt > 1:622335/1‑69 (MQ=255) tGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAg < 1:3250687/71‑1 (MQ=255) ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc > 1:2167885/1‑71 (MQ=255) ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc > 1:143374/1‑71 (MQ=255) ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc > 1:1002513/1‑71 (MQ=255) aTGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg < 1:1840271/60‑1 (MQ=255) tGGCTGTTGGTGAACAGCATTATCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg > 1:1916970/1‑71 (MQ=255) tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg > 1:2915610/1‑59 (MQ=255) tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg > 1:205013/1‑71 (MQ=255) tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCACAGACCTGCTGTCGGCGGTAAGCCATg > 1:1699821/1‑71 (MQ=255) ggCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:2707742/53‑1 (MQ=255) ggCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa < 1:1754461/68‑1 (MQ=255) gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt < 1:1736708/70‑1 (MQ=255) cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc < 1:1157779/71‑1 (MQ=255) tGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc > 1:608211/1‑70 (MQ=255) tGTTGGTGAACAGCATTACCATACCGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt > 1:389763/1‑66 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa < 1:3740287/70‑1 (MQ=255) tGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgctg < 1:3630332/48‑1 (MQ=255) ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg > 1:1557379/1‑71 (MQ=255) gTGAACAGCATTACCATACTGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg < 1:81702/70‑1 (MQ=255) gTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg < 1:1431549/70‑1 (MQ=255) tGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg < 1:126706/71‑1 (MQ=255) aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag < 1:3533722/70‑3 (MQ=255) cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa > 1:2858227/1‑55 (MQ=255) aGCATTACCATACGGTCGATACCAATTCCCAGACCTTCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa < 1:950687/70‑1 (MQ=255) gCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACg > 1:1010793/1‑71 (MQ=255) cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACg > 1:448310/1‑70 (MQ=255) cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGt < 1:2788785/71‑1 (MQ=255) ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:2087148/56‑1 (MQ=255) ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTg < 1:836498/65‑1 (MQ=255) ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa > 1:198306/1‑71 (MQ=255) tACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg < 1:164329/41‑1 (MQ=255) tACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg < 1:277304/41‑1 (MQ=255) tACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAAt > 1:3450901/1‑71 (MQ=255) aatcATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAAt > 1:1777609/4‑71 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt > 1:306058/1‑51 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt > 1:2295852/1‑51 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAAt < 1:3606931/70‑1 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc > 1:427271/1‑71 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc > 1:2344956/1‑71 (MQ=255) cATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt < 1:3122925/71‑1 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa < 1:221560/71‑1 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTg < 1:1049062/57‑1 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt < 1:2233645/70‑1 (MQ=255) cGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTcgt < 1:353542/70‑3 (MQ=255) ggTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg < 1:2583459/71‑1 (MQ=255) gTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt < 1:2972254/71‑1 (MQ=255) tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCgg < 1:1605072/52‑1 (MQ=255) gATACCAATTCCCAGACTTGCTGTCGGCTGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa < 1:3717702/71‑1 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTg > 1:3552068/1‑52 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg < 1:3441203/67‑1 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:508248/1‑70 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:1807213/1‑70 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:1471501/1‑70 (MQ=255) aTACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa < 1:458509/71‑1 (MQ=255) aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa < 1:549750/71‑1 (MQ=255) aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa < 1:132951/71‑1 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt > 1:829145/1‑37 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAAc > 1:2634888/1‑70 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa > 1:3843692/1‑71 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa > 1:1356223/1‑49 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:2041472/1‑71 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:548675/1‑71 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:14951/1‑71 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:2469833/1‑71 (MQ=255) ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt < 1:1395080/71‑1 (MQ=255) cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt < 1:405118/52‑1 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACTTAATCTTCATCGTAGAACATCGCTccg < 1:1694504/71‑1 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg < 1:923091/71‑1 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg < 1:3455080/71‑1 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt > 1:23360/1‑52 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:224271/1‑63 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:1968959/1‑71 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:2562153/71‑1 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:606744/1‑71 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:1605980/1‑71 (MQ=255) gACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCg > 1:618821/1‑63 (MQ=255) gACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtcg > 1:3809992/1‑71 (MQ=255) ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc < 1:1069018/71‑1 (MQ=255) ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt > 1:3359588/1‑51 (MQ=255) ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg < 1:1392547/53‑1 (MQ=255) tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt > 1:1142805/1‑50 (MQ=255) tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGt > 1:3108360/1‑50 (MQ=255) tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATTGCTtcgtc > 1:1449694/1‑66 (MQ=255) tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:491385/1‑66 (MQ=255) tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:1406876/1‑66 (MQ=255) gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc > 1:776171/1‑68 (MQ=255) gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAcc > 1:101896/1‑71 (MQ=255) ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAAc > 1:3646882/1‑53 (MQ=255) ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCt < 1:2406103/71‑1 (MQ=255) tgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTg > 1:3416936/1‑71 (MQ=255) | TAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTG > minE/1932957‑1933097 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |