| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,189 | A→G | 12.0% | I418I (ATT→ATC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,189 | 0 | A | G | 12.0% | 216.7 / 18.7 | 83 | I418I (ATT→ATC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (36/37); new base G (5/5); total (41/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
AGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCT > minE/1933121‑1933258 | aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt > 1:1174807/1‑71 (MQ=255) aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg > 1:2422718/1‑69 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTTAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag < 1:3100201/71‑1 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag < 1:588970/71‑1 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg > 1:2820078/1‑68 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg > 1:811197/1‑68 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg > 1:2028881/1‑68 (MQ=255) gTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga < 1:1899667/66‑1 (MQ=255) ttGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACt < 1:2981290/71‑1 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTc > 1:2710334/1‑70 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTc > 1:1515216/1‑70 (MQ=255) cgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCaa < 1:3357953/71‑1 (MQ=255) cTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg < 1:2190432/58‑1 (MQ=255) tGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACg > 1:2342460/1‑71 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg < 1:3537468/57‑1 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTc < 1:969005/65‑1 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg > 1:1873221/1‑71 (MQ=255) gATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACTACCAATGAAGAACTCAAAGCgg > 1:3859767/1‑71 (MQ=255) gATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCaaa < 1:3848389/67‑1 (MQ=255) gATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCgg > 1:3929879/1‑71 (MQ=255) aTCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCgg > 1:1730122/1‑70 (MQ=255) tCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc > 1:3757949/1‑71 (MQ=255) ttCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATgaagaa < 1:2467497/57‑1 (MQ=255) ttCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaaga > 1:569602/1‑56 (MQ=255) ttCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt > 1:1161900/1‑70 (MQ=255) tCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt > 1:3499349/1‑71 (MQ=255) tCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt > 1:3914162/1‑71 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt > 1:2034309/1‑70 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtg < 1:1455201/69‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtg < 1:1450719/69‑1 (MQ=255) cgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAtt < 1:2907455/71‑1 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCaaa > 1:836713/1‑54 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTcc < 1:1689755/70‑1 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTcc < 1:2887362/70‑1 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACAACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCg < 1:703898/71‑1 (MQ=255) ttCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt < 1:191498/60‑1 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGAccacca > 1:1118770/1‑38 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGAccacca > 1:3274912/1‑38 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg > 1:2316328/1‑71 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg > 1:2238909/1‑71 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCgg > 1:2220784/1‑70 (MQ=255) cAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGt > 1:3167449/1‑71 (MQ=255) aaGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGt > 1:3550012/2‑71 (MQ=255) aGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGt < 1:3040635/70‑1 (MQ=255) gCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTa < 1:2474462/71‑1 (MQ=255) gCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTa < 1:1854008/71‑1 (MQ=255) ttCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGtt < 1:3888445/68‑1 (MQ=255) cTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:2049958/58‑1 (MQ=255) cTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:2149629/58‑1 (MQ=255) tCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAc > 1:3572752/1‑71 (MQ=255) tCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTaa > 1:488429/1‑70 (MQ=255) cGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAt > 1:229169/1‑58 (MQ=255) cGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcg > 1:1839803/1‑71 (MQ=255) gCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcg < 1:313528/70‑1 (MQ=255) cTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt < 1:1599480/49‑1 (MQ=255) cTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtc < 1:2897852/71‑1 (MQ=255) tAAAGCCGTTACCGATTTCACGACCACCAATgaag < 1:829082/35‑1 (MQ=255) tAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtcg > 1:1676638/1‑71 (MQ=255) aaaGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt > 1:199218/1‑49 (MQ=255) aaGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtcgt < 1:770120/70‑1 (MQ=255) ccattACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacg > 1:3628617/4‑71 (MQ=255) ccGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacg < 1:3698967/71‑1 (MQ=255) attACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacgg > 1:3154713/2‑69 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGa > 1:2209484/1‑44 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGa > 1:1841800/1‑44 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacgac > 1:3619747/1‑68 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc < 1:368352/70‑1 (MQ=255) cGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcg < 1:2706932/56‑1 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:670838/71‑1 (MQ=255) aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg > 1:3506037/1‑71 (MQ=255) aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg > 1:788704/1‑71 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc < 1:3943499/71‑1 (MQ=255) tttCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:2355954/38‑1 (MQ=255) ttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:3070692/1‑66 (MQ=255) tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1834786/70‑1 (MQ=255) tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:2078351/70‑1 (MQ=255) cACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCgg < 1:1340749/70‑1 (MQ=255) aCGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTa > 1:2210341/1‑55 (MQ=255) aCGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGga < 1:2053879/70‑1 (MQ=255) gACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGggga > 1:3336015/1‑70 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1943883/66‑1 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:2000826/70‑1 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:1762637/70‑1 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgag > 1:2691720/1‑69 (MQ=255) accaccAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaaa < 1:489697/71‑1 (MQ=255) ccaccgATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTaa > 1:1583561/1‑43 (MQ=25) ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac < 1:1077931/52‑1 (MQ=255) caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCg < 1:3872940/36‑1 (MQ=255) caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:2885904/70‑1 (MQ=255) caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACt < 1:986997/71‑1 (MQ=255) caATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt < 1:1541563/71‑1 (MQ=255) | AGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCT > minE/1933121‑1933258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |