| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 309,867 | T→A | 11.5% | A1002A (GCA→GCT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 309,867 | 0 | T | A | 11.5% | 208.4 / 17.6 | 78 | A1002A (GCA→GCT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (36/33); new base A (5/4); total (41/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAG > minE/309798‑309935 | gAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTg > 1:1474655/1‑71 (MQ=255) acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg < 1:3082222/69‑1 (MQ=255) aTACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCg < 1:350623/71‑1 (MQ=255) aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:1227198/71‑1 (MQ=255) ccGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTc < 1:5099/71‑1 (MQ=255) ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGtt < 1:912952/70‑1 (MQ=255) cGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCt < 1:756989/71‑1 (MQ=255) gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc > 1:3107272/1‑71 (MQ=255) taCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcg > 1:350509/2‑71 (MQ=255) aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc > 1:1502455/1‑71 (MQ=255) aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc < 1:3699715/71‑1 (MQ=255) aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc < 1:3761026/71‑1 (MQ=255) aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcg > 1:2198179/1‑70 (MQ=255) aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGGTCTgcgc < 1:1468783/71‑1 (MQ=255) cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg < 1:2391592/71‑1 (MQ=255) gacgaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc < 1:3693684/68‑1 (MQ=255) gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc < 1:844117/71‑1 (MQ=255) gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc > 1:2518292/1‑71 (MQ=255) gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg < 1:1516309/71‑1 (MQ=255) gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCg > 1:432992/1‑70 (MQ=255) aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg > 1:343039/1‑70 (MQ=255) aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg > 1:1533154/1‑70 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc < 1:1058779/71‑1 (MQ=255) ttGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCa < 1:1277295/71‑1 (MQ=255) tGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg > 1:2240384/1‑71 (MQ=255) gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc > 1:3017790/1‑69 (MQ=255) gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc > 1:2411339/1‑69 (MQ=255) tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGcc > 1:2374236/1‑57 (MQ=255) tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGcc > 1:3569415/1‑57 (MQ=255) aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCa > 1:467581/1‑69 (MQ=255) aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg < 1:447574/70‑1 (MQ=255) cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTc < 1:2388672/46‑1 (MQ=255) cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTAc < 1:3538927/71‑1 (MQ=255) cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTAc > 1:1971779/1‑71 (MQ=255) cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTAc > 1:291112/1‑71 (MQ=255) ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc < 1:1483380/63‑1 (MQ=255) gCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGa > 1:2202386/1‑70 (MQ=255) cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt < 1:1876620/70‑1 (MQ=255) ggTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg < 1:2328175/42‑1 (MQ=255) ggTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg < 1:1274505/42‑1 (MQ=255) tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCt < 1:1327094/39‑1 (MQ=255) tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCt < 1:3047510/39‑1 (MQ=255) tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc > 1:328478/1‑70 (MQ=255) tCACCATCCC‑CCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAcc < 1:155341/57‑1 (MQ=255) cACCATCCCGCCCATTACACCGGTACCTACTGCGTTc > 1:1549396/1‑37 (MQ=255) cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg > 1:2778931/1‑59 (MQ=255) cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc > 1:2954398/1‑69 (MQ=255) cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc > 1:3761830/1‑69 (MQ=255) aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACt < 1:3247268/62‑1 (MQ=255) aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCa < 1:3657933/70‑1 (MQ=255) aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCa > 1:927027/1‑70 (MQ=255) aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg > 1:1082288/1‑71 (MQ=255) aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg > 1:18761/1‑71 (MQ=255) cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCTGAACCAGCACCAGTACTGATAACCAGCg < 1:3407678/71‑1 (MQ=255) cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg > 1:1425086/1‑71 (MQ=255) cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg > 1:3805356/1‑71 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGCGTTCTgcg > 1:2616681/1‑36 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCgg > 1:2289047/1‑70 (MQ=255) cccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa > 1:1801455/1‑71 (MQ=255) ccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg < 1:409043/67‑1 (MQ=255) cccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATaa > 1:1930723/1‑70 (MQ=255) cccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAAc > 1:2299343/1‑71 (MQ=255) cccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAAc > 1:3348179/1‑71 (MQ=255) ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt < 1:1395343/55‑1 (MQ=255) aTTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc > 1:1702184/1‑71 (MQ=255) acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc > 1:3937843/1‑40 (MQ=255) acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTa > 1:1573387/1‑45 (MQ=255) acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGa < 1:607219/70‑1 (MQ=255) acCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa < 1:771483/71‑1 (MQ=255) acCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa < 1:3418941/71‑1 (MQ=255) ccGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGAt < 1:2760635/71‑1 (MQ=255) ccGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAg > 1:2664856/1‑68 (MQ=255) ccGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAg > 1:1643547/1‑68 (MQ=255) cgatACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGa > 1:1048890/4‑66 (MQ=255) gTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaa < 1:83574/70‑1 (MQ=255) cTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGc > 1:194374/1‑70 (MQ=255) cTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCa < 1:3408444/69‑1 (MQ=255) cTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAg < 1:2832088/70‑1 (MQ=255) tGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg > 1:3653521/1‑44 (MQ=255) tGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGcc > 1:1207920/1‑67 (MQ=255) | GAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAG > minE/309798‑309935 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |