| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,240 | C→T | 31.6% | A401A (GCG→GCA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,240 | 0 | C | T | 31.6% | 79.4 / 43.4 | 60 | A401A (GCG→GCA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (11/30); new base T (7/12); total (18/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCC > minE/1933171‑1933306 | aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc < 1:368352/70‑1 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:670838/71‑1 (MQ=255) aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg > 1:788704/1‑71 (MQ=255) aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg > 1:3506037/1‑71 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc < 1:3943499/71‑1 (MQ=255) ttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:3070692/1‑66 (MQ=255) tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1834786/70‑1 (MQ=255) tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:2078351/70‑1 (MQ=255) cACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCgg < 1:1340749/70‑1 (MQ=255) aCGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGga < 1:2053879/70‑1 (MQ=255) gACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGggga > 1:3336015/1‑70 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1943883/66‑1 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:1762637/70‑1 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:2000826/70‑1 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgag > 1:2691720/1‑69 (MQ=255) accaccAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaaa < 1:489697/71‑1 (MQ=255) caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:2885904/70‑1 (MQ=255) caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACt < 1:986997/71‑1 (MQ=255) cgaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGgggac < 1:2261431/63‑2 (MQ=255) caATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt < 1:1541563/71‑1 (MQ=255) gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTg < 1:990147/70‑1 (MQ=255) gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTg < 1:2143574/70‑1 (MQ=255) gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg > 1:767057/1‑71 (MQ=255) gaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa > 1:3235564/1‑70 (MQ=255) attCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGatat < 1:1320466/69‑1 (MQ=255) cTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc < 1:3357586/43‑1 (MQ=255) cTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATAtt < 1:3411527/71‑1 (MQ=255) cAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg < 1:722270/64‑1 (MQ=255) cAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATAtt < 1:704346/69‑1 (MQ=255) aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTc < 1:1405310/69‑1 (MQ=255) aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCgg > 1:809687/1‑46 (MQ=255) aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCgg > 1:3497264/1‑46 (MQ=255) gCGGTCTGTGATTTCCGGGTTAACGGCGTTACGACGCGCCAGCGgaga < 1:2889304/48‑1 (MQ=255) cggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGata > 1:245831/1‑62 (MQ=255) cGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGga < 1:3150566/45‑1 (MQ=255) ggTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg < 1:147228/71‑1 (MQ=255) ggTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc < 1:2229912/41‑1 (MQ=19) gTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGa < 1:1878731/71‑1 (MQ=255) gTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAAtaa < 1:3803418/71‑3 (MQ=255) tCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:2258177/40‑1 (MQ=255) tCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1183831/40‑1 (MQ=255) tCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt < 1:2256603/49‑1 (MQ=255) cggtgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAg > 1:2340843/3‑71 (MQ=255) tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGa < 1:1264421/55‑1 (MQ=255) gcgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTaa < 1:3846345/62‑1 (MQ=255) gATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGga > 1:2496929/1‑37 (MQ=255) aTTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTgg > 1:3352935/1‑71 (MQ=255) ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg > 1:2317851/1‑70 (MQ=255) ggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt < 1:892255/61‑1 (MQ=255) ggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:3920323/1‑70 (MQ=255) gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:1595899/1‑69 (MQ=255) ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCg < 1:3165632/61‑1 (MQ=255) ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAg < 1:1366373/71‑1 (MQ=255) ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAg < 1:867695/71‑1 (MQ=255) cgtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgt < 1:971606/71‑1 (MQ=255) gtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgtg > 1:3734586/1‑71 (MQ=255) tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGAATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc < 1:2590603/71‑1 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc > 1:1879522/1‑69 (MQ=255) ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAtg < 1:544849/65‑1 (MQ=255) tACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCt > 1:1157883/1‑71 (MQ=255) acggcgTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCt < 1:1208896/70‑1 (MQ=255) gacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGcc < 1:2321248/71‑1 (MQ=255) acgCGCCATCGGAGAAACTTCTGCCGGATATTCAg < 1:405096/35‑1 (MQ=255) | ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCC > minE/1933171‑1933306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |