| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 310,779 | C→G | 10.1% | A698A (GCG→GCC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 310,779 | 0 | C | G | 10.1% | 221.6 / 11.6 | 79 | A698A (GCG→GCC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (37/34); new base G (4/4); total (41/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTA > minE/310709‑310848 | ccAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgc > 1:13703/1‑71 (MQ=255) gACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTAGCTGCTTCTGCAAGCAACTGGTTACGCGcc > 1:1639666/1‑71 (MQ=255) gACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTGCGCTGCTTCTGCAAGCAACTGGTTACGCGcc < 1:14495/71‑1 (MQ=255) aCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGcc < 1:2369559/70‑1 (MQ=255) aCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCt > 1:3424576/1‑71 (MQ=255) ccGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCt > 1:377911/1‑70 (MQ=255) cGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa < 1:1890409/71‑1 (MQ=255) gTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAg > 1:3103774/1‑71 (MQ=255) tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGt < 1:2958128/71‑1 (MQ=255) tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGt < 1:162940/71‑1 (MQ=255) tGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGt > 1:2213759/1‑69 (MQ=255) tGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGt > 1:439135/1‑69 (MQ=255) ggACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACgcgc < 1:634323/62‑1 (MQ=255) ggACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAg > 1:3130390/1‑71 (MQ=255) aCGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtt < 1:2614093/71‑1 (MQ=255) cGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt > 1:2941970/1‑71 (MQ=255) tACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAg < 1:2650051/62‑1 (MQ=255) cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgc > 1:1071104/1‑53 (MQ=255) cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc < 1:2918122/70‑1 (MQ=255) cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg > 1:3228633/1‑71 (MQ=255) aaCATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg > 1:3483735/1‑71 (MQ=255) aCATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGGCTGAGt < 1:2706700/52‑1 (MQ=255) atatCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc > 1:2914019/1‑70 (MQ=255) cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCaa < 1:996557/63‑1 (MQ=255) ggTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg < 1:2500475/53‑1 (MQ=255) ggTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg < 1:827407/53‑1 (MQ=255) ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCaa < 1:1884911/55‑1 (MQ=255) ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa < 1:1746145/69‑1 (MQ=255) ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:2655032/1‑71 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa > 1:3455283/1‑38 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa > 1:2907207/1‑38 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGa > 1:711949/1‑49 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt < 1:1078909/69‑1 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:3141402/1‑69 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:2062369/1‑69 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc < 1:2051395/70‑1 (MQ=255) gctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCt < 1:48507/61‑1 (MQ=255) gctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg < 1:646128/71‑1 (MQ=255) ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg < 1:575452/44‑1 (MQ=255) ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg < 1:1094045/44‑1 (MQ=255) gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtt > 1:813155/1‑37 (MQ=255) gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtt > 1:3344638/1‑37 (MQ=255) gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt > 1:3763047/1‑70 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt < 1:901874/69‑1 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt < 1:471647/69‑1 (MQ=255) tCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc > 1:1954965/1‑68 (MQ=255) tGCAAGCAACTTGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt > 1:316966/1‑71 (MQ=255) accaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagt > 1:3579898/3‑71 (MQ=255) accaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagt > 1:351289/3‑71 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc > 1:2114190/2‑71 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc > 1:2573173/2‑71 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc < 1:1314353/70‑1 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagt < 1:2486406/69‑1 (MQ=255) caagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcg < 1:2758862/71‑2 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca < 1:2711687/71‑1 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca < 1:3896191/71‑1 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc > 1:2867036/1‑70 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc > 1:3703668/1‑70 (MQ=255) aagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga < 1:396480/71‑3 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTg < 1:3422555/36‑1 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg < 1:3277726/51‑1 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg > 1:3301772/1‑60 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg > 1:92915/1‑60 (MQ=255) agcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaac > 1:2726355/1‑70 (MQ=255) agcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca > 1:905232/1‑69 (MQ=255) gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag > 1:829777/1‑71 (MQ=255) aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCaa < 1:1002894/37‑1 (MQ=255) aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:2924668/71‑1 (MQ=255) aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:1383708/71‑1 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACTAAGGCCAGCCTGGTCAATCAGc > 1:2284306/1‑55 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGc > 1:480434/1‑55 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg < 1:3873835/71‑1 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg < 1:3215487/71‑1 (MQ=255) tGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa < 1:2366504/48‑1 (MQ=255) ggTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa > 1:399158/1‑38 (MQ=255) ggTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa > 1:983871/1‑38 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa > 1:2292176/1‑37 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt < 1:3880562/69‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt < 1:1244384/69‑1 (MQ=255) tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGcc > 1:1734052/1‑38 (MQ=255) tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg > 1:2920819/1‑69 (MQ=255) tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg > 1:637619/1‑69 (MQ=255) gcgcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt < 1:913837/54‑1 (MQ=255) gcgcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTa < 1:365271/71‑1 (MQ=255) cgcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc > 1:3863507/1‑43 (MQ=255) | CCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTA > minE/310709‑310848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |