| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,282 | C→T | 33.7% | P387P (CCG→CCA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,282 | 0 | C | T | 33.7% | 97.8 / 75.5 | 87 | P387P (CCG→CCA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (25/32); new base T (14/15); total (39/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
ATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCC > minE/1933214‑1933346 | aTTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTgg > 1:3352935/1‑71 (MQ=255) ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg > 1:2317851/1‑70 (MQ=255) ggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:3920323/1‑70 (MQ=255) gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:1595899/1‑69 (MQ=255) ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCg < 1:3165632/61‑1 (MQ=255) ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAg < 1:867695/71‑1 (MQ=255) ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAg < 1:1366373/71‑1 (MQ=255) cgtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgt < 1:971606/71‑1 (MQ=255) gtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgtg > 1:3734586/1‑71 (MQ=255) tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGAATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc < 1:2590603/71‑1 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc > 1:1879522/1‑69 (MQ=255) ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAtg < 1:544849/65‑1 (MQ=255) tACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCt > 1:1157883/1‑71 (MQ=255) acggcgTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCt < 1:1208896/70‑1 (MQ=255) gacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGcc < 1:2321248/71‑1 (MQ=255) gtgcCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCAct < 1:3935828/69‑1 (MQ=255) cAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtctt > 1:3056262/1‑71 (MQ=255) cAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtctt > 1:1575206/1‑71 (MQ=255) cAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtctt < 1:1288964/71‑1 (MQ=255) cAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtctt < 1:2490657/71‑1 (MQ=255) aGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACttcttc < 1:2505579/71‑1 (MQ=255) gCGGGGACACTTCTGCCGTATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatca < 1:1798835/71‑2 (MQ=255) gCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatca < 1:1455906/71‑2 (MQ=255) gCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatc > 1:2304842/1‑70 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAg < 1:3688637/49‑1 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACttcttc > 1:3209953/1‑70 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:2743481/71‑1 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:1282024/71‑1 (MQ=255) cGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCActt < 1:1325994/65‑1 (MQ=255) cGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa < 1:3332325/71‑1 (MQ=255) ggAGAAACTTCTGCCGTATATTCTTTAATGAAGGTGGGCTGAATCAGAtg < 1:1280261/50‑1 (MQ=255) gaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCa > 1:2147195/1‑58 (MQ=255) aaaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTc < 1:3325177/52‑1 (MQ=255) aaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtct < 1:3191140/71‑1 (MQ=255) aCTTCTGCCGGATATTCCGTAATAAAGGGTGGCTGAATCAGATGTGCTTCTGCCActtca < 1:1423155/60‑2 (MQ=255) aCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa < 1:3497932/40‑1 (MQ=255) aCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa < 1:1739470/40‑1 (MQ=255) cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:1180128/62‑1 (MQ=255) cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg < 1:677601/71‑1 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCt < 1:837524/49‑1 (MQ=38) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatc > 1:3214535/1‑60 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctctg > 1:3427040/1‑69 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctctg > 1:1462838/1‑69 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctctg > 1:1793457/1‑69 (MQ=255) ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCgg > 1:546764/1‑71 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa < 1:3571730/37‑1 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGa > 1:2275243/1‑39 (MQ=255) cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGt > 1:1528363/1‑70 (MQ=255) cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGt < 1:3167784/70‑1 (MQ=255) cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt > 1:324878/1‑70 (MQ=255) tGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTaa > 1:1404275/1‑71 (MQ=255) gCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCAc > 1:3494584/1‑50 (MQ=255) gCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtct > 1:1932959/1‑54 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTa < 1:2387211/68‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTa < 1:1810719/68‑1 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAca < 1:2562765/70‑2 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAc < 1:3765587/69‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCt < 1:2843124/43‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGACACTTCTTCGAAGATCTCGGTAACGa > 1:831199/1‑71 (MQ=255) ggATATTCCGTAATAAAGGTTGGCTGAATCAGAtg < 1:3517823/35‑1 (MQ=25) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc < 1:1248171/38‑1 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGa < 1:2126397/58‑1 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:3495522/1‑70 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:1592644/1‑70 (MQ=255) gATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa < 1:3900767/71‑1 (MQ=255) tatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCt < 1:3785542/39‑1 (MQ=255) tatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACg > 1:436776/1‑71 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtctc < 1:2358898/56‑1 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:3371050/1‑71 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATa > 1:489336/3‑65 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActt > 1:3497586/1‑42 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActt > 1:3913153/1‑42 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActt > 1:3545151/1‑42 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:1451068/1‑63 (MQ=255) cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatc < 1:2671391/44‑1 (MQ=255) aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc < 1:3691115/39‑1 (MQ=255) tAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:3327095/44‑1 (MQ=255) aaTAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCaacc > 1:522311/1‑68 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc < 1:3098484/71‑1 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:2327411/1‑70 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:2167486/1‑70 (MQ=255) aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGtga < 1:1770045/52‑3 (MQ=255) aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCA‑Acccc > 1:815524/1‑69 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc < 1:3802313/70‑1 (MQ=255) aGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:1087968/1‑47 (MQ=255) aGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:783357/1‑47 (MQ=255) gTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtct < 1:2675862/43‑1 (MQ=255) | ATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCC > minE/1933214‑1933346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |