| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,312 | T→A | 18.2% | E377D (GAA→GAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,312 | 0 | T | A | 18.2% | 173.4 / 33.4 | 77 | E377D (GAA→GAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (29/34); new base A (8/6); total (37/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
CAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCA > minE/1933243‑1933381 | cAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtctt > 1:3056262/1‑71 (MQ=255)cAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtctt > 1:1575206/1‑71 (MQ=255)cAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtctt < 1:1288964/71‑1 (MQ=255)cAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtctt < 1:2490657/71‑1 (MQ=255) aGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACttcttc < 1:2505579/71‑1 (MQ=255) gCGGGGACACTTCTGCCGTATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatca < 1:1798835/71‑2 (MQ=255) gCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatca < 1:1455906/71‑2 (MQ=255) gCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatc > 1:2304842/1‑70 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACttcttc > 1:3209953/1‑70 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:2743481/71‑1 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:1282024/71‑1 (MQ=255) cGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa < 1:3332325/71‑1 (MQ=255) aaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtct < 1:3191140/71‑1 (MQ=255) cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:1180128/62‑1 (MQ=255) cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg < 1:677601/71‑1 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatc > 1:3214535/1‑60 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctctg > 1:3427040/1‑69 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctctg > 1:1462838/1‑69 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctctg > 1:1793457/1‑69 (MQ=255) ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCgg > 1:546764/1‑71 (MQ=255) cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGt > 1:1528363/1‑70 (MQ=255) cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGt < 1:3167784/70‑1 (MQ=255) cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt > 1:324878/1‑70 (MQ=255) tGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTaa > 1:1404275/1‑71 (MQ=255) gCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtct > 1:1932959/1‑54 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTa < 1:2387211/68‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTa < 1:1810719/68‑1 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAca < 1:2562765/70‑2 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAc < 1:3765587/69‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGACACTTCTTCGAAGATCTCGGTAACGa > 1:831199/1‑71 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGa < 1:2126397/58‑1 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:3495522/1‑70 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:1592644/1‑70 (MQ=255) gATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa < 1:3900767/71‑1 (MQ=255) tatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACg > 1:436776/1‑71 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtctc < 1:2358898/56‑1 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:3371050/1‑71 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATa > 1:489336/3‑65 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:1451068/1‑63 (MQ=255) cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatc < 1:2671391/44‑1 (MQ=255) tAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:3327095/44‑1 (MQ=255) aaTAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCaacc > 1:522311/1‑68 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc < 1:3098484/71‑1 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:2327411/1‑70 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:2167486/1‑70 (MQ=255) aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGtga < 1:1770045/52‑3 (MQ=255) aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCA‑Acccc > 1:815524/1‑69 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc < 1:3802313/70‑1 (MQ=255) aGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:783357/1‑47 (MQ=255) aGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:1087968/1‑47 (MQ=255) gTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtct < 1:2675862/43‑1 (MQ=255) ttggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGctt < 1:3338806/69‑2 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:2603798/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:854070/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa < 1:999637/70‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa < 1:2218973/70‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAAc < 1:2021833/71‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGct < 1:1377821/61‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACg < 1:952308/71‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACg < 1:934048/71‑1 (MQ=255) cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:1336831/1‑68 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAg > 1:2657762/1‑57 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGctct > 1:958536/1‑61 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGt < 1:1039940/70‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg > 1:634405/1‑71 (MQ=255) aGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAccg < 1:3288293/70‑3 (MQ=255) aTGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc < 1:91851/45‑1 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:1051086/1‑36 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:3281668/37‑1 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:3084274/1‑71 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:3077592/1‑71 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGTCCCAGACCCCAGCTCTTCTCAACGTGGATGCCg < 1:2445001/71‑1 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:830991/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCg < 1:2270213/71‑1 (MQ=255) ttCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:2435912/1‑46 (MQ=255) tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa > 1:3042568/1‑70 (MQ=255) tCTGCCACTTATTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:136297/1‑44 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtctt > 1:12180/1‑52 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa > 1:375866/1‑69 (MQ=255) gCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg > 1:644045/1‑59 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:755818/52‑1 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:1017272/52‑1 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCg < 1:398359/65‑1 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAg < 1:2631763/69‑1 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:3622973/71‑1 (MQ=255) cACTTCTTCGAAGATCTCGGTAACTATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt < 1:2911318/71‑1 (MQ=255) cACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAAc > 1:899521/1‑54 (MQ=255) cACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa > 1:1345345/1‑65 (MQ=255) aCTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:2278324/1‑70 (MQ=255) cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAAc > 1:3102110/1‑52 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:1293342/65‑1 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc > 1:1524940/1‑70 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGa < 1:2562539/63‑1 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCa > 1:1968865/1‑70 (MQ=255) | CAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCA > minE/1933243‑1933381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |