| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,327 | A→G | 10.2% | V372V (GTT→GTC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,327 | 0 | A | G | 10.2% | 210.2 / 13.0 | 78 | V372V (GTT→GTC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (37/33); new base G (4/4); total (41/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
TGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCA > minE/1933258‑1933393 | tGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTaa > 1:1404275/1‑71 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTa < 1:2387211/68‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTa < 1:1810719/68‑1 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAca < 1:2562765/70‑2 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAc < 1:3765587/69‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGACACTTCTTCGAAGATCTCGGTAACGa > 1:831199/1‑71 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:1592644/1‑70 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:3495522/1‑70 (MQ=255) gATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa < 1:3900767/71‑1 (MQ=255) tatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACg > 1:436776/1‑71 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:3371050/1‑71 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATa > 1:489336/3‑65 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:1451068/1‑63 (MQ=255) aaTAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCaacc > 1:522311/1‑68 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc < 1:3098484/71‑1 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:2327411/1‑70 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:2167486/1‑70 (MQ=255) aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCA‑Acccc > 1:815524/1‑69 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc < 1:3802313/70‑1 (MQ=255) ttggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGctt < 1:3338806/69‑2 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:2603798/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:854070/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa < 1:999637/70‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa < 1:2218973/70‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAAc < 1:2021833/71‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGct < 1:1377821/61‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACg < 1:952308/71‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACg < 1:934048/71‑1 (MQ=255) cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:1336831/1‑68 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAg > 1:2657762/1‑57 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGctct > 1:958536/1‑61 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGt < 1:1039940/70‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg > 1:634405/1‑71 (MQ=255) aGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAccg < 1:3288293/70‑3 (MQ=255) aTGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc < 1:91851/45‑1 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:1051086/1‑36 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:3281668/37‑1 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:3084274/1‑71 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:3077592/1‑71 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGTCCCAGACCCCAGCTCTTCTCAACGTGGATGCCg < 1:2445001/71‑1 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:830991/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCg < 1:2270213/71‑1 (MQ=255) ttCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:2435912/1‑46 (MQ=255) tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa > 1:3042568/1‑70 (MQ=255) tCTGCCACTTATTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:136297/1‑44 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtctt > 1:12180/1‑52 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa > 1:375866/1‑69 (MQ=255) gCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg > 1:644045/1‑59 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:1017272/52‑1 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:755818/52‑1 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCg < 1:398359/65‑1 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAg < 1:2631763/69‑1 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:3622973/71‑1 (MQ=255) cACTTCTTCGAAGATCTCGGTAACTATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt < 1:2911318/71‑1 (MQ=255) cACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAAc > 1:899521/1‑54 (MQ=255) cACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa > 1:1345345/1‑65 (MQ=255) aCTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:2278324/1‑70 (MQ=255) cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAAc > 1:3102110/1‑52 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:1293342/65‑1 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc > 1:1524940/1‑70 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGa < 1:2562539/63‑1 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCa > 1:1968865/1‑70 (MQ=255) tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTc > 1:872003/1‑65 (MQ=255) cGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg > 1:3761292/1‑49 (MQ=255) gAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAtt > 1:3411652/1‑70 (MQ=255) aaGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGc > 1:1393313/1‑71 (MQ=255) gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt < 1:2615521/71‑1 (MQ=255) gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt < 1:3576120/71‑1 (MQ=255) aTCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCa > 1:1632895/1‑60 (MQ=255) ctcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACg < 1:1922717/40‑1 (MQ=255) ctcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACg < 1:2088080/40‑1 (MQ=255) ctcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGAt > 1:3912951/1‑51 (MQ=255) ctcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCaa > 1:2139955/1‑62 (MQ=255) tcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg > 1:1590480/1‑70 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:2143175/67‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg > 1:1344165/1‑68 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGc < 1:505269/69‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa < 1:920000/70‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa < 1:924726/70‑1 (MQ=255) gTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGc < 1:1315475/43‑1 (MQ=255) gTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTCCTTTCGCa > 1:3709521/1‑69 (MQ=255) | TGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCA > minE/1933258‑1933393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |