Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,567 C→T 11.9% E292E (GAG→GAA lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,5670CT11.9% 276.2 / 21.9 101E292E (GAG→GAAlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (44/45);  new base T (6/6);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 102 total aligned reads displayed.

GTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGT  >  minE/1933498‑1933636
                                                                     |                                                                     
gTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCt                                                                      >  1:1583329/1‑71 (MQ=255)
 tCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTc                                                                     <  1:612638/71‑1 (MQ=255)
   gCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCt                                                                      <  1:1114222/68‑1 (MQ=255)
   gCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGa                                                                   <  1:1798249/71‑1 (MQ=255)
   gCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGa                                                                   >  1:1602227/1‑71 (MQ=255)
     cGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGAt                                                                  >  1:2935580/1‑70 (MQ=255)
         ggTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGAt                                                                  >  1:1501163/1‑66 (MQ=255)
           tCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                                           <  1:2661728/71‑1 (MQ=255)
           tCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                                           <  1:1821559/71‑1 (MQ=255)
           tCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                                           <  1:1711308/71‑1 (MQ=255)
            cACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt                                                          >  1:3284334/1‑71 (MQ=255)
             aCTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCg                                                                    <  1:182154/60‑1 (MQ=255)
               ttCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGa                                                                   <  1:1496774/59‑1 (MQ=255)
               ttCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                                           <  1:1979196/67‑1 (MQ=255)
               ttCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                                           <  1:528870/67‑1 (MQ=255)
                 ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                                           >  1:1112316/1‑65 (MQ=255)
                 ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa                                                     >  1:3661312/1‑71 (MQ=255)
                 ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa                                                     >  1:1313704/1‑71 (MQ=255)
                 ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa                                                     >  1:544646/1‑71 (MQ=255)
                   gTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATc                                                   >  1:871269/1‑71 (MQ=255)
                    tCTTACCGAGAATATCCTGTGCCAGAGTACTGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt                                                  <  1:1710787/71‑1 (MQ=255)
                      ttACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGa                                                                   >  1:612403/1‑52 (MQ=255)
                      ttACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc                                                >  1:2458388/1‑71 (MQ=255)
                        aCCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGt                                                       <  1:2297364/62‑1 (MQ=255)
                         ccGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTa                                             <  1:2822034/71‑1 (MQ=255)
                          cGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGAt                                                                  >  1:3484164/1‑49 (MQ=255)
                            agaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc                                          >  1:1038735/1‑71 (MQ=255)
                            agaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc                                          >  1:1486025/1‑71 (MQ=255)
                                tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGt                                                       >  1:2745682/1‑54 (MQ=255)
                                tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAAt                                                    >  1:823861/1‑57 (MQ=255)
                                tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAg                                           >  1:2768729/1‑66 (MQ=255)
                                tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg                                      >  1:1074683/1‑71 (MQ=255)
                                tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg                                      >  1:768477/1‑71 (MQ=255)
                                tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg                                      <  1:519088/71‑1 (MQ=255)
                                  tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg                                      <  1:174480/69‑1 (MQ=255)
                                  tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg                                      >  1:2619424/1‑69 (MQ=255)
                                  tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg                                      >  1:425492/1‑69 (MQ=255)
                                  tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa                                    <  1:1605697/71‑1 (MQ=255)
                                  tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa                                    <  1:3829995/71‑1 (MQ=255)
                                   ccTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc                                                >  1:730151/1‑58 (MQ=255)
                                     tgtgCCAGAGTACGGAACAGCGATTCGGTCAGCTCg                                                                    <  1:2083726/36‑1 (MQ=255)
                                      gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt                                >  1:2980744/1‑71 (MQ=255)
                                      gtgCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGt                                >  1:1470473/1‑71 (MQ=255)
                                       tgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGtt                               <  1:1240079/71‑1 (MQ=255)
                                       tgCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt                               >  1:1981415/1‑71 (MQ=255)
                                         ccAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTc                              >  1:1078007/1‑70 (MQ=255)
                                         ccAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg                                      <  1:786839/62‑1 (MQ=255)
                                          cAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGAt                                                             <  1:2258319/38‑1 (MQ=255)
                                          cAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                                           <  1:1820228/40‑1 (MQ=255)
                                           agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat                           >  1:1726141/1‑71 (MQ=255)
                                           agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat                           >  1:1115578/1‑71 (MQ=255)
                                            gagTACGGAACAGCGATTCGGTCAGCTCGATCAGATca                                                           >  1:909922/1‑37 (MQ=255)
                                             agTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                                           <  1:738327/37‑1 (MQ=255)
                                             agTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa                                                      <  1:1207152/42‑1 (MQ=255)
                                               tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa                                                      <  1:1514177/40‑1 (MQ=255)
                                               tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt                                                  <  1:3159745/44‑1 (MQ=255)
                                                gcGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcattg                      >  1:1729220/2‑69 (MQ=255)
                                                 cGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcattg                      <  1:90056/70‑3 (MQ=255)
                                                 cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag                                   <  1:3330462/57‑1 (MQ=255)
                                                 cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg                      <  1:546070/70‑1 (MQ=255)
                                                 cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt                     >  1:2260033/1‑71 (MQ=255)
                                                  ggAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg                    <  1:1985606/71‑1 (MQ=255)
                                                  ggAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg                    <  1:3179668/71‑1 (MQ=255)
                                                  ggAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg                      <  1:2105788/69‑1 (MQ=255)
                                                  ggAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt                     >  1:1987943/1‑70 (MQ=255)
                                                   gAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCAt                                       <  1:3551587/51‑1 (MQ=255)
                                                   gAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCAt                                       <  1:2437724/51‑1 (MQ=255)
                                                   gAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCAt                                       <  1:3466422/51‑1 (MQ=255)
                                                    aaCAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGaa                  >  1:3827449/1‑71 (MQ=255)
                                                    aaCAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGaa                  >  1:1244421/1‑71 (MQ=255)
                                                       aGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCg                                               <  1:3917914/39‑1 (MQ=255)
                                                        gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct              <  1:3855310/71‑1 (MQ=255)
                                                        gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct              <  1:2216259/71‑1 (MQ=255)
                                                         cGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGTGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTg             <  1:1743573/71‑1 (MQ=255)
                                                          gATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTaa                                            >  1:1856602/1‑39 (MQ=255)
                                                          gATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActc               >  1:2322600/1‑68 (MQ=255)
                                                          gATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg            >  1:485489/1‑71 (MQ=255)
                                                            ttCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca                         <  1:2949102/56‑1 (MQ=255)
                                                            ttCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca                         <  1:2876671/56‑1 (MQ=255)
                                                            ttCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct              <  1:1983370/67‑1 (MQ=255)
                                                            ttCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGt          >  1:3195258/1‑71 (MQ=255)
                                                            atCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct              <  1:3503072/66‑1 (MQ=255)
                                                             tCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg                       >  1:2884053/1‑57 (MQ=255)
                                                             tCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg                       >  1:146770/1‑57 (MQ=255)
                                                              cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa        >  1:3364686/1‑71 (MQ=255)
                                                              cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa        >  1:1169826/1‑71 (MQ=255)
                                                              cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa        >  1:777507/1‑71 (MQ=255)
                                                                gTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt                               <  1:3207729/46‑1 (MQ=255)
                                                                 tCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTAt       <  1:607760/69‑1 (MQ=255)
                                                                 tCAGCTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATg      <  1:2451787/70‑1 (MQ=255)
                                                                  cAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGaa                  >  1:2092152/1‑57 (MQ=255)
                                                                  cAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGaa                  >  1:3043963/1‑57 (MQ=255)
                                                                  cAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTa        <  1:3128010/67‑1 (MQ=255)
                                                                  cAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGAc    >  1:3286051/1‑71 (MQ=255)
                                                                   aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg   >  1:2663657/1‑71 (MQ=255)
                                                                    gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg   >  1:1850673/1‑70 (MQ=255)
                                                                    gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt  <  1:1877632/71‑1 (MQ=255)
                                                                    gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt  <  1:3933959/71‑1 (MQ=255)
                                                                    gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt  <  1:3595634/71‑1 (MQ=255)
                                                                     cTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGt          <  1:290444/62‑1 (MQ=255)
                                                                     |                                                                     
GTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGT  >  minE/1933498‑1933636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: