| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,591 | G→A | 14.3% | Y284Y (TAC→TAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,591 | 0 | G | A | 14.3% | 267.0 / 30.2 | 105 | Y284Y (TAC→TAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (47/43); new base A (7/8); total (54/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
CCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAG > minE/1933523‑1933660 | ccGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTa < 1:2822034/71‑1 (MQ=255) agaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc > 1:1486025/1‑71 (MQ=255) agaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc > 1:1038735/1‑71 (MQ=255) tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAg > 1:2768729/1‑66 (MQ=255) tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:1074683/1‑71 (MQ=255) tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:768477/1‑71 (MQ=255) tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg < 1:519088/71‑1 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg < 1:174480/69‑1 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:425492/1‑69 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa < 1:1605697/71‑1 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa < 1:3829995/71‑1 (MQ=255) gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt > 1:2980744/1‑71 (MQ=255) tgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGtt < 1:1240079/71‑1 (MQ=255) tgCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt > 1:1981415/1‑71 (MQ=255) ccAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTc > 1:1078007/1‑70 (MQ=255) ccAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg < 1:786839/62‑1 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat > 1:1115578/1‑71 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat > 1:1726141/1‑71 (MQ=255) gcGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcattg > 1:1729220/2‑69 (MQ=255) cGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcattg < 1:90056/70‑3 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag < 1:3330462/57‑1 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg < 1:546070/70‑1 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:2260033/1‑71 (MQ=255) ggAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:3179668/71‑1 (MQ=255) ggAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg < 1:2105788/69‑1 (MQ=255) ggAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:1987943/1‑70 (MQ=255) gAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCAt < 1:3466422/51‑1 (MQ=255) gAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCAt < 1:3551587/51‑1 (MQ=255) gAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCAt < 1:2437724/51‑1 (MQ=255) aaCAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGaa > 1:3827449/1‑71 (MQ=255) aaCAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGaa > 1:1244421/1‑71 (MQ=255) aGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCg < 1:3917914/39‑1 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:2216259/71‑1 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:3855310/71‑1 (MQ=255) cGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGTGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTg < 1:1743573/71‑1 (MQ=255) gATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTaa > 1:1856602/1‑39 (MQ=255) gATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActc > 1:2322600/1‑68 (MQ=255) gATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg > 1:485489/1‑71 (MQ=255) ttCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca < 1:2949102/56‑1 (MQ=255) ttCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca < 1:2876671/56‑1 (MQ=255) ttCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:1983370/67‑1 (MQ=255) ttCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGt > 1:3195258/1‑71 (MQ=255) atCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:3503072/66‑1 (MQ=255) tCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg > 1:2884053/1‑57 (MQ=255) tCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg > 1:146770/1‑57 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:777507/1‑71 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:3364686/1‑71 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:1169826/1‑71 (MQ=255) gTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt < 1:3207729/46‑1 (MQ=255) tCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTAt < 1:607760/69‑1 (MQ=255) tCAGCTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATg < 1:2451787/70‑1 (MQ=255) cAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGaa > 1:2092152/1‑57 (MQ=255) cAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGaa > 1:3043963/1‑57 (MQ=255) cAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTa < 1:3128010/67‑1 (MQ=255) cAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGAc > 1:3286051/1‑71 (MQ=255) aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:2663657/1‑71 (MQ=255) gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:1850673/1‑70 (MQ=255) gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt < 1:3595634/71‑1 (MQ=255) gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt < 1:1877632/71‑1 (MQ=255) gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt < 1:3933959/71‑1 (MQ=255) cTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGt < 1:290444/62‑1 (MQ=255) tCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatcat < 1:1587113/47‑1 (MQ=255) tCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca > 1:1441675/1‑46 (MQ=255) tCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca > 1:2345360/1‑46 (MQ=255) caaTCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcat > 1:3939105/3‑46 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACgg < 1:3446948/71‑1 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg > 1:3195083/1‑70 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg > 1:3189364/1‑70 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg > 1:1757474/1‑70 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg < 1:1206485/70‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGAAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:1078116/71‑1 (MQ=255) aTCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:3543291/71‑1 (MQ=255) tCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaa < 1:666948/71‑1 (MQ=255) tCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa > 1:3618664/1‑70 (MQ=255) cAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTGCATCATGGTGAACTCTGGGTTATGACGTACGGaa < 1:1139601/70‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:1231389/1‑70 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAc < 1:240769/46‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTggg > 1:2315991/1‑52 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTggg > 1:1431123/1‑52 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTg < 1:1558585/50‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGt < 1:623521/53‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2270945/66‑1 (MQ=255) cTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:3423575/1‑66 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2408485/63‑1 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCtt > 1:1431638/1‑71 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCtt > 1:1810435/1‑71 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg > 1:2147321/1‑47 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTc > 1:3648812/1‑71 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTc > 1:3713919/1‑71 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTc > 1:3911701/1‑71 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCg > 1:2566959/1‑71 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGtt < 1:3654142/71‑1 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAc > 1:1800792/1‑71 (MQ=255) tCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGtt < 1:1603635/68‑1 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt > 1:2204062/1‑57 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:1432986/1‑70 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg < 1:3763661/68‑1 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg < 1:3747255/68‑1 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa < 1:2727350/70‑1 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAg < 1:1151388/71‑1 (MQ=255) | CCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAG > minE/1933523‑1933660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |