| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,699 | C→A | 16.6% | V248V (GTG→GTT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,699 | 0 | C | A | 16.6% | 185.5 / 16.4 | 66 | V248V (GTG→GTT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (31/24); new base A (7/4); total (38/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
TGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGG > minE/1933631‑1933768 | tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:1300805/71‑1 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccacc < 1:3086059/70‑1 (MQ=255) cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac > 1:1376230/1‑68 (MQ=255) cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac > 1:3630647/1‑68 (MQ=255) aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg > 1:3649797/1‑71 (MQ=255) cagAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAg > 1:629623/3‑67 (MQ=255) cagAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgt > 1:3141714/3‑70 (MQ=255) cGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGc > 1:2166419/1‑71 (MQ=255) ggAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCt > 1:2088152/1‑71 (MQ=255) ggAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCt > 1:1701368/1‑71 (MQ=255) agAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgtt < 1:3260411/70‑3 (MQ=255) agAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgtt < 1:1161142/70‑3 (MQ=255) gAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt > 1:2154592/1‑71 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGAc < 1:3478955/66‑1 (MQ=255) cTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:2315807/71‑1 (MQ=255) ttCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa > 1:3514976/1‑69 (MQ=255) tcattACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAg > 1:3712641/4‑71 (MQ=255) cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGt > 1:2433687/1‑71 (MQ=255) attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTAcaacca < 1:3163864/52‑1 (MQ=255) ttACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCt < 1:3123190/58‑1 (MQ=255) ttACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc < 1:1908235/71‑1 (MQ=255) tACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGa > 1:1414325/1‑53 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc < 1:495593/70‑1 (MQ=255) ggAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgc > 1:623995/1‑71 (MQ=255) gAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcg < 1:792838/71‑1 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:2790031/44‑1 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCTGACGCTTGAGGTACAGTTCCGgcgc < 1:2946860/71‑1 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:2645146/1‑71 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:3912495/1‑71 (MQ=255) aGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:1843283/1‑70 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:1967341/42‑1 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACAAGACGCTTGAGGTACAGTTCCGGCGCGa > 1:1364136/1‑71 (MQ=255) aCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATAc > 1:3718751/1‑71 (MQ=255) cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATAc < 1:1133437/70‑1 (MQ=255) cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg < 1:616068/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg > 1:1168678/1‑70 (MQ=255) gTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCa < 1:1078146/71‑1 (MQ=255) ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:3474315/69‑1 (MQ=255) ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg > 1:3080684/1‑71 (MQ=255) ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg > 1:3582482/1‑71 (MQ=255) gATTTCGAATACACGCTCGAAGCCACCAACCACCAGa > 1:2640767/1‑37 (MQ=255) gATTTCGAATACACGCTAGAAGCCACCAACCACCAGa > 1:1152665/1‑37 (MQ=255) aTTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa > 1:3416192/1‑71 (MQ=255) aTTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa < 1:2983683/71‑1 (MQ=255) tttCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATa > 1:911576/1‑70 (MQ=255) ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc > 1:1181936/1‑52 (MQ=255) ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:3801662/1‑71 (MQ=255) ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:1286058/1‑71 (MQ=255) ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:1290634/1‑71 (MQ=255) cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt < 1:3335801/70‑1 (MQ=255) cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt < 1:638973/70‑1 (MQ=255) cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATg > 1:1181427/1‑71 (MQ=255) gAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGt > 1:587896/1‑71 (MQ=255) gAATACACGCTCGAAGCCACCAACCACCAGACGCTT‑AGGTACAGTTCCGgcgc < 1:339026/53‑1 (MQ=255) aaTACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGt < 1:1217053/70‑1 (MQ=255) tACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATcta < 1:2333770/71‑3 (MQ=255) tACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATct > 1:1983765/1‑69 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg < 1:3768193/42‑1 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc > 1:1214196/1‑69 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga < 1:86483/71‑1 (MQ=255) acacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa < 1:2422356/62‑1 (MQ=255) cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga < 1:3669173/71‑1 (MQ=255) gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc < 1:1093881/71‑1 (MQ=255) gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc > 1:893998/1‑70 (MQ=255) tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg < 1:2364790/40‑1 (MQ=255) tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg > 1:571229/1‑71 (MQ=255) cGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga > 1:126340/1‑65 (MQ=255) aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg > 1:2184303/1‑71 (MQ=255) gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt < 1:2949407/71‑1 (MQ=255) ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATa > 1:3723678/1‑42 (MQ=255) ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt > 1:3802675/1‑70 (MQ=255) caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg < 1:3878224/46‑1 (MQ=255) caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg < 1:1007358/46‑1 (MQ=255) accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATAc < 1:489179/41‑1 (MQ=255) ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGAt < 1:2319111/58‑1 (MQ=255) caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg > 1:1888811/1‑71 (MQ=255) caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg < 1:2314149/71‑1 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga > 1:3477225/1‑55 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAAGTACATGTCGAGATCCAGCGCGTTAtggt > 1:2978053/1‑71 (MQ=255) accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtg > 1:135577/1‑68 (MQ=255) ccaccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtgg < 1:2061418/71‑1 (MQ=255) ccaccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtgg < 1:1134267/71‑1 (MQ=255) ccaccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtgg < 1:2897533/71‑1 (MQ=255) | TGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGG > minE/1933631‑1933768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |