Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,714 G→A 12.2% Y243Y (TAC→TAT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,7140GA12.2% 221.4 / 17.5 82Y243Y (TAC→TATlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (36/36);  new base A (7/3);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACG  >  minE/1933647‑1933782
                                                                   |                                                                    
cTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc                                                                   <  1:2315807/71‑1 (MQ=255)
 ttCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                                    >  1:3514976/1‑69 (MQ=255)
  tcattACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAg                                                                 >  1:3712641/4‑71 (MQ=255)
   cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGt                                                                >  1:2433687/1‑71 (MQ=255)
     ttACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc                                                              <  1:1908235/71‑1 (MQ=255)
       aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc                                                             <  1:495593/70‑1 (MQ=255)
         ggAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgc                                                          >  1:623995/1‑71 (MQ=255)
          gAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcg                                                         <  1:792838/71‑1 (MQ=255)
           aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCTGACGCTTGAGGTACAGTTCCGgcgc                                                        <  1:2946860/71‑1 (MQ=255)
           aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                                        >  1:3912495/1‑71 (MQ=255)
           aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                                        >  1:2645146/1‑71 (MQ=255)
            aGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                                        >  1:1843283/1‑70 (MQ=255)
             gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACAAGACGCTTGAGGTACAGTTCCGGCGCGa                                                      >  1:1364136/1‑71 (MQ=255)
                aCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATAc                                                   >  1:3718751/1‑71 (MQ=255)
                 cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATAc                                                   <  1:1133437/70‑1 (MQ=255)
                 cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg                                                  <  1:616068/71‑1 (MQ=255)
                  ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg                                                  >  1:1168678/1‑70 (MQ=255)
                   gTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCa                                                <  1:1078146/71‑1 (MQ=255)
                    ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                                 <  1:3474315/69‑1 (MQ=255)
                    ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg                                               >  1:3080684/1‑71 (MQ=255)
                    ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg                                               >  1:3582482/1‑71 (MQ=255)
                       aTTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                            >  1:3416192/1‑71 (MQ=255)
                       aTTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                            <  1:2983683/71‑1 (MQ=255)
                        tttCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATa                                            >  1:911576/1‑70 (MQ=255)
                         ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc                                                             >  1:1181936/1‑52 (MQ=255)
                         ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa                                          >  1:1286058/1‑71 (MQ=255)
                         ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa                                          >  1:1290634/1‑71 (MQ=255)
                         ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa                                          >  1:3801662/1‑71 (MQ=255)
                           cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt                                         <  1:3335801/70‑1 (MQ=255)
                           cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt                                         <  1:638973/70‑1 (MQ=255)
                           cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATg                                        >  1:1181427/1‑71 (MQ=255)
                            gAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGt                                       >  1:587896/1‑71 (MQ=255)
                            gAATACACGCTCGAAGCCACCAACCACCAGACGCTTAGGTACAGTTCCGgcgc                                                        <  1:339026/53‑1 (MQ=255)
                             aaTACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGt                                       <  1:1217053/70‑1 (MQ=255)
                               tACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATcta                                    <  1:2333770/71‑3 (MQ=255)
                               tACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATct                                     >  1:1983765/1‑69 (MQ=255)
                               tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg                                                                 <  1:3768193/42‑1 (MQ=255)
                               tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc                                      >  1:1214196/1‑69 (MQ=255)
                               tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                                    <  1:86483/71‑1 (MQ=255)
                                acacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                            <  1:2422356/62‑1 (MQ=255)
                                 cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga                                  <  1:3669173/71‑1 (MQ=255)
                                    gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc                               <  1:1093881/71‑1 (MQ=255)
                                    gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc                                >  1:893998/1‑70 (MQ=255)
                                      tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg                                                            <  1:2364790/40‑1 (MQ=255)
                                      tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg                             >  1:571229/1‑71 (MQ=255)
                                       cGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga                                  >  1:126340/1‑65 (MQ=255)
                                          aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                         >  1:2184303/1‑71 (MQ=255)
                                           gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                        <  1:2949407/71‑1 (MQ=255)
                                            ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATa                                                    >  1:3723678/1‑42 (MQ=255)
                                            ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                        >  1:3802675/1‑70 (MQ=255)
                                             caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg                                               <  1:3878224/46‑1 (MQ=255)
                                             caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg                                               <  1:1007358/46‑1 (MQ=255)
                                              accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATAc                                                   <  1:489179/41‑1 (MQ=255)
                                               ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGAt                                 <  1:2319111/58‑1 (MQ=255)
                                                caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                   <  1:2314149/71‑1 (MQ=255)
                                                caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                   >  1:1888811/1‑71 (MQ=255)
                                                 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga                                  >  1:3477225/1‑55 (MQ=255)
                                                 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAAGTACATGTCGAGATCCAGCGCGTTAtggt                  >  1:2978053/1‑71 (MQ=255)
                                                  accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtg                    >  1:135577/1‑68 (MQ=255)
                                                   ccaccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtgg                <  1:2061418/71‑1 (MQ=255)
                                                   ccaccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtgg                <  1:1134267/71‑1 (MQ=255)
                                                   ccaccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtgg                <  1:2897533/71‑1 (MQ=255)
                                                   caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                >  1:1558106/3‑71 (MQ=255)
                                                      ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCg                                     >  1:3657825/1‑47 (MQ=255)
                                                      ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCg                                     >  1:1844443/1‑47 (MQ=255)
                                                      ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt              >  1:3460855/1‑70 (MQ=255)
                                                      ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt              >  1:3239038/1‑70 (MQ=255)
                                                       caGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                            <  1:1366743/39‑1 (MQ=255)
                                                       caGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa            >  1:446239/1‑71 (MQ=255)
                                                        aGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCAAGCGCGTTATGGTGGGTGa            >  1:3154480/1‑70 (MQ=255)
                                                         gACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAAt           >  1:97730/1‑70 (MQ=255)
                                                         gACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAAt           >  1:649542/1‑70 (MQ=255)
                                                         gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                                    <  1:2850073/45‑1 (MQ=255)
                                                          aCGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAt                                         <  1:770260/39‑1 (MQ=16)
                                                          aCGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa          >  1:2535162/1‑70 (MQ=255)
                                                            gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                     <  1:3047046/57‑1 (MQ=255)
                                                            gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                     <  1:3645636/57‑1 (MQ=255)
                                                            gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                     <  1:654660/57‑1 (MQ=255)
                                                            gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                     <  1:3002532/57‑1 (MQ=255)
                                                                gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa        >  1:2801156/1‑66 (MQ=255)
                                                                gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg     <  1:1638514/69‑1 (MQ=255)
                                                                gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg     <  1:1513571/69‑1 (MQ=255)
                                                                  ggTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcg  >  1:782838/1‑70 (MQ=255)
                                                                   gTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                        <  1:3457676/47‑1 (MQ=255)
                                                                   gTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                        <  1:1438502/47‑1 (MQ=255)
                                                                   |                                                                    
CTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACG  >  minE/1933647‑1933782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: