Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,765 G→A 10.0% H226H (CAC→CAT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,7650GA10.0% 140.3 / 5.6 50H226H (CAC→CATlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base A (3/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

CAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATA  >  minE/1933695‑1933834
                                                                      |                                                                     
caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                                       <  1:2314149/71‑1 (MQ=255)
caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                                       >  1:1888811/1‑71 (MQ=255)
 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAAGTACATGTCGAGATCCAGCGCGTTAtggt                                                                      >  1:2978053/1‑71 (MQ=255)
   ccaccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtgg                                                                    <  1:2897533/71‑1 (MQ=255)
   ccaccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtgg                                                                    <  1:1134267/71‑1 (MQ=255)
   ccaccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtgg                                                                    <  1:2061418/71‑1 (MQ=255)
   caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                                                                    >  1:1558106/3‑71 (MQ=255)
      ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                                  >  1:3460855/1‑70 (MQ=255)
      ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                                  >  1:3239038/1‑70 (MQ=255)
       caGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                                                >  1:446239/1‑71 (MQ=255)
        aGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCAAGCGCGTTATGGTGGGTGa                                                                >  1:3154480/1‑70 (MQ=255)
         gACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAAt                                                               >  1:649542/1‑70 (MQ=255)
         gACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAAt                                                               >  1:97730/1‑70 (MQ=255)
          aCGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                              >  1:2535162/1‑70 (MQ=255)
                gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                                            >  1:2801156/1‑66 (MQ=255)
                gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                                         <  1:1638514/69‑1 (MQ=255)
                gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                                         <  1:1513571/69‑1 (MQ=255)
                  ggTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcg                                                      >  1:782838/1‑70 (MQ=255)
                  gatACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcga                                                     <  1:3758614/69‑2 (MQ=255)
                     aCAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                                    >  1:1354442/1‑69 (MQ=255)
                      cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                                 >  1:2361368/1‑71 (MQ=255)
                      cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                                 >  1:1155071/1‑71 (MQ=255)
                       aGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGc                                                <  1:1819896/71‑1 (MQ=255)
                        gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg                                               >  1:1475851/1‑71 (MQ=255)
                        gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg                                               <  1:3654387/71‑1 (MQ=255)
                         ttCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                                         <  1:1904697/60‑1 (MQ=255)
                          tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                                              >  1:3460421/1‑70 (MQ=255)
                           ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtggg                                                                   <  1:2377535/48‑1 (MQ=255)
                             ggCGCGATACGCATGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaa                                                             <  1:2995049/52‑1 (MQ=255)
                             ggCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaa                                                             <  1:3497882/52‑1 (MQ=255)
                             ggCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                                         <  1:1727812/56‑1 (MQ=255)
                              gcgcGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                                                      <  1:610453/42‑1 (MQ=255)
                               cgcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                                              <  1:982465/49‑1 (MQ=255)
                                gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                                       <  1:2264892/71‑1 (MQ=255)
                                gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                                       <  1:2126745/71‑1 (MQ=255)
                                gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGc                                        >  1:3251873/1‑70 (MQ=255)
                                  gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                                     <  1:3706680/71‑1 (MQ=255)
                                  gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                                     <  1:1788250/71‑1 (MQ=255)
                                    tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                                    >  1:1957852/1‑70 (MQ=255)
                                     aCGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                                  >  1:453053/1‑71 (MQ=255)
                                      cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                                  <  1:880894/70‑1 (MQ=255)
                                       gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                                  <  1:3910733/69‑1 (MQ=255)
                                         aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc                              >  1:516773/1‑71 (MQ=255)
                                           gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                                    <  1:2381602/63‑1 (MQ=255)
                                           gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt                            >  1:2798574/1‑71 (MQ=255)
                                           gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt                            >  1:1026421/1‑71 (MQ=255)
                                               aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAc                                                       >  1:1075801/1‑40 (MQ=255)
                                               aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATc                                <  1:3224735/63‑1 (MQ=255)
                                                 gTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa                                            >  1:1257388/1‑49 (MQ=255)
                                                   cGAGCTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa                                            >  1:431986/1‑47 (MQ=255)
                                                   cGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAcc                                          >  1:1575550/1‑49 (MQ=255)
                                                   cGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCatcatc                    >  1:2247221/1‑71 (MQ=255)
                                                    gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCg                   <  1:1550066/71‑1 (MQ=255)
                                                        tCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCg                <  1:214493/70‑1 (MQ=255)
                                                          cAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGttt             <  1:3709489/71‑1 (MQ=255)
                                                           aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGttt             >  1:1721954/1‑70 (MQ=255)
                                                           aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc            <  1:3296067/71‑1 (MQ=255)
                                                            gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCg                                               <  1:3218267/35‑1 (MQ=255)
                                                             cgcgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCaa          >  1:2246622/1‑71 (MQ=255)
                                                                attATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACtt       >  1:3654041/2‑71 (MQ=255)
                                                                  tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc      >  1:3135061/1‑70 (MQ=255)
                                                                    tggtggGCGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCAt   <  1:3850904/71‑1 (MQ=255)
                                                                      gtgggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATa  >  1:2177956/1‑70 (MQ=255)
                                                                      |                                                                     
CAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATA  >  minE/1933695‑1933834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: