| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,788 | C→A | 11.8% | A219S (GCT→TCT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,788 | 0 | C | A | 11.8% | 185.7 / 8.0 | 59 | A219S (GCT→TCT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (28/24); new base A (3/4); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTG > minE/1933718‑1933857 | aGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGc < 1:1819896/71‑1 (MQ=255) gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg > 1:1475851/1‑71 (MQ=255) gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg < 1:3654387/71‑1 (MQ=255) tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg > 1:3460421/1‑70 (MQ=255) gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc < 1:2264892/71‑1 (MQ=255) gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc < 1:2126745/71‑1 (MQ=255) gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGc > 1:3251873/1‑70 (MQ=255) gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg < 1:3706680/71‑1 (MQ=255) gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg < 1:1788250/71‑1 (MQ=255) tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg > 1:1957852/1‑70 (MQ=255) aCGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa > 1:453053/1‑71 (MQ=255) cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa < 1:880894/70‑1 (MQ=255) gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa < 1:3910733/69‑1 (MQ=255) aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc > 1:516773/1‑71 (MQ=255) gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg < 1:2381602/63‑1 (MQ=255) gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt > 1:2798574/1‑71 (MQ=255) gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt > 1:1026421/1‑71 (MQ=255) aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATc < 1:3224735/63‑1 (MQ=255) gTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa > 1:1257388/1‑49 (MQ=255) cGAGCTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa > 1:431986/1‑47 (MQ=255) cGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAcc > 1:1575550/1‑49 (MQ=255) cGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCatcatc > 1:2247221/1‑71 (MQ=255) gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCg < 1:1550066/71‑1 (MQ=255) tCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCg < 1:214493/70‑1 (MQ=255) cAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGttt < 1:3709489/71‑1 (MQ=255) aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGttt > 1:1721954/1‑70 (MQ=255) aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc < 1:3296067/71‑1 (MQ=255) gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCg < 1:3218267/35‑1 (MQ=255) cgcgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCaa > 1:2246622/1‑71 (MQ=255) attATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACtt > 1:3654041/2‑71 (MQ=255) tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc > 1:3135061/1‑70 (MQ=255) tggtggGCGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCAt < 1:3850904/71‑1 (MQ=255) tgatgggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTcc > 1:2541611/4‑69 (MQ=255) gtgggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATa > 1:2177956/1‑70 (MQ=255) atgggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATa < 1:2810000/69‑1 (MQ=255) atgggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATa < 1:3696467/69‑1 (MQ=255) tgggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATa > 1:1411182/1‑69 (MQ=255) gggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAg > 1:1414807/1‑71 (MQ=255) ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAg < 1:2314223/70‑1 (MQ=255) ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGc > 1:3260724/1‑71 (MQ=255) gtgATAAACGGACGCGCAGCGGCTCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACt > 1:1454886/1‑60 (MQ=255) tgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatcat < 1:1989731/46‑1 (MQ=255) aTAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgc < 1:3420029/70‑1 (MQ=255) tAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCgg > 1:2073661/1‑71 (MQ=255) tAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCgg > 1:3834172/1‑71 (MQ=255) aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgcg > 1:269631/1‑69 (MQ=255) aaaCGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGt > 1:333916/1‑71 (MQ=255) aaaCCGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCgg > 1:2977140/1‑45 (MQ=255) aaCGGACGCGCAGCGGCACCGCCAGGGATCACCTGcat > 1:1976026/1‑38 (MQ=255) aaCGGACGCGCAGCGGCACCGCCAGGGATCACCTGcat > 1:232042/1‑38 (MQ=255) aaCGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGtt < 1:3052754/71‑1 (MQ=255) gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:3683957/71‑1 (MQ=255) gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:1859456/71‑1 (MQ=255) gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:1816317/71‑1 (MQ=255) gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:1877772/71‑1 (MQ=255) gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAcc < 1:1664655/71‑1 (MQ=255) aCGCGCAGCGGCACCGCCAGGGATCACGTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCa > 1:1597963/1‑71 (MQ=255) cgcgCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTcc > 1:2770204/1‑51 (MQ=255) gcgcAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCAt < 1:1095457/70‑1 (MQ=255) gCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTg < 1:550273/71‑1 (MQ=255) | AGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTG > minE/1933718‑1933857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |