| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,945 | G→A | 17.4% | R166R (CGC→CGT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,945 | 0 | G | A | 17.4% | 144.1 / 22.1 | 63 | R166R (CGC→CGT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (26/26); new base A (9/2); total (35/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.29e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGC > minE/1933876‑1934014 | cTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgc > 1:1650185/1‑71 (MQ=255)cTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgc > 1:3567198/1‑71 (MQ=255)cTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAg > 1:663269/1‑70 (MQ=255)cTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAg > 1:2342910/1‑70 (MQ=255) gcgcACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCtt > 1:2034617/1‑71 (MQ=255) gcgcACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCtt > 1:2889091/1‑71 (MQ=255) aCTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCt > 1:1491716/1‑70 (MQ=255) aCTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCt > 1:1833314/1‑70 (MQ=255) aCTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCt > 1:1618405/1‑70 (MQ=255) aCTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTg < 1:2649665/71‑1 (MQ=255) tAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATc < 1:1473676/70‑1 (MQ=255) tAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATc < 1:2915678/70‑1 (MQ=255) tAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATc < 1:2278985/70‑1 (MQ=255) gtgtTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGc > 1:624358/1‑69 (MQ=255) gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCtt > 1:3413519/1‑53 (MQ=255) gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt > 1:3877097/1‑71 (MQ=255) gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATACTGCAAGCCg < 1:1179262/70‑1 (MQ=255) cGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt < 1:3171502/70‑1 (MQ=255) gggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATc > 1:3075143/1‑58 (MQ=255) gggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATc > 1:3622749/1‑58 (MQ=255) gggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATc > 1:2168081/1‑58 (MQ=255) ggATTCTTCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt < 1:805195/68‑1 (MQ=255) ggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt < 1:2804304/68‑1 (MQ=255) ggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgg > 1:3499569/1‑70 (MQ=255) ttCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGcc < 1:1034422/63‑1 (MQ=255) ttCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAtt > 1:3209376/1‑71 (MQ=255) ttaTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAtt > 1:606104/3‑70 (MQ=255) aTCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt > 1:3705092/1‑71 (MQ=255) gTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc < 1:1311780/70‑1 (MQ=255) gTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc < 1:2830040/70‑1 (MQ=255) ttGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCgg < 1:3227969/71‑1 (MQ=255) cgatgaggtCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTggta > 1:2147575/2‑67 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg > 1:1843248/1‑71 (MQ=255) atgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa < 1:1865289/55‑1 (MQ=255) atgagatCGAGATAACGCTGACAATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt > 1:1278203/1‑61 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCt < 1:1945349/41‑1 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCaa < 1:1774235/45‑1 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATc > 1:210819/1‑59 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg > 1:1281842/1‑67 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg > 1:2977955/1‑71 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATcc < 1:1401615/37‑1 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAtt < 1:1400419/53‑1 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAg < 1:486779/63‑1 (MQ=255) gagaTAACGCTGACGATAGCGCGCTTCCTGATCATGCa > 1:1880361/1‑38 (MQ=255) agaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGt > 1:1346340/1‑69 (MQ=255) agaTAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAttt < 1:366453/50‑1 (MQ=255) gaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACgt > 1:2572066/1‑64 (MQ=255) aTAACGCTGACGATAGCGCGCTTCCTGAGCCTGCAAGCCGTGGAATTTATCCGGc < 1:2164308/55‑1 (MQ=255) tAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtt > 1:1584586/1‑70 (MQ=255) aaCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTa > 1:2402927/1‑47 (MQ=255) aaCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTa > 1:2165279/1‑47 (MQ=255) aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta < 1:3901868/70‑2 (MQ=255) aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta < 1:3022798/70‑2 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCCGCGGACGCAGTGCTTTgg < 1:1927494/71‑1 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg < 1:3189770/70‑1 (MQ=255) cGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgg < 1:2426346/39‑1 (MQ=255) cGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt > 1:3601980/1‑71 (MQ=255) gCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt < 1:3547776/70‑1 (MQ=255) tGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag > 1:119124/1‑71 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACgta > 1:3114064/1‑55 (MQ=255) cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagcag < 1:2671373/71‑1 (MQ=255) cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag > 1:2228930/1‑68 (MQ=255) gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt > 1:1524875/1‑39 (MQ=255) gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt > 1:3690804/1‑39 (MQ=255) gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt > 1:1529379/1‑39 (MQ=255) gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa < 1:1193225/71‑1 (MQ=255) tAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTc > 1:2968468/1‑63 (MQ=255) gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc < 1:1660476/70‑1 (MQ=255) | CTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGC > minE/1933876‑1934014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |