Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 310,794 T→A 10.0% E693D (GAA→GAT acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE310,7940TA10.0% 217.8 / 13.3 80E693D (GAA→GATacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (34/38);  new base A (4/4);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATT  >  minE/310727‑310863
                                                                   |                                                                     
cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                                                     <  1:2918122/70‑1 (MQ=255)
cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                                    >  1:3228633/1‑71 (MQ=255)
        aaCATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                                                            >  1:3483735/1‑71 (MQ=255)
           atatCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                                          >  1:2914019/1‑70 (MQ=255)
               cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCaa                                                             <  1:996557/63‑1 (MQ=255)
                  ggTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                                    <  1:827407/53‑1 (MQ=255)
                  ggTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                                    <  1:2500475/53‑1 (MQ=255)
                       ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCaa                                                             <  1:1884911/55‑1 (MQ=255)
                       ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa                                               <  1:1746145/69‑1 (MQ=255)
                       ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                             >  1:2655032/1‑71 (MQ=255)
                         cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGa                                                                 >  1:711949/1‑49 (MQ=255)
                         cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                             <  1:1078909/69‑1 (MQ=255)
                         cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                             >  1:3141402/1‑69 (MQ=255)
                         cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                             >  1:2062369/1‑69 (MQ=255)
                         cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc                                            <  1:2051395/70‑1 (MQ=255)
                          gctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCt                                                    <  1:48507/61‑1 (MQ=255)
                          gctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                                          <  1:646128/71‑1 (MQ=255)
                           ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                                    <  1:1094045/44‑1 (MQ=255)
                           ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                                    <  1:575452/44‑1 (MQ=255)
                             gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                                        >  1:3763047/1‑70 (MQ=255)
                              ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                                        <  1:901874/69‑1 (MQ=255)
                              ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                                        <  1:471647/69‑1 (MQ=255)
                                tCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc                                       >  1:1954965/1‑68 (MQ=255)
                                  tGCAAGCAACTTGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt                                  >  1:316966/1‑71 (MQ=255)
                                  accaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagt                                  >  1:351289/3‑71 (MQ=255)
                                  accaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagt                                  >  1:3579898/3‑71 (MQ=255)
                                   ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc                                 <  1:1314353/70‑1 (MQ=255)
                                   ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc                                 >  1:2573173/2‑71 (MQ=255)
                                   ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc                                 >  1:2114190/2‑71 (MQ=255)
                                   ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagt                                  <  1:2486406/69‑1 (MQ=255)
                                    caagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcg                                <  1:2758862/71‑2 (MQ=255)
                                    caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca                                <  1:2711687/71‑1 (MQ=255)
                                    caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca                                <  1:3896191/71‑1 (MQ=255)
                                    caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc                                 >  1:2867036/1‑70 (MQ=255)
                                    caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc                                 >  1:3703668/1‑70 (MQ=255)
                                     aagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga                               <  1:396480/71‑3 (MQ=255)
                                     aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTg                                                                  <  1:3422555/36‑1 (MQ=255)
                                     aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg                                                   <  1:3277726/51‑1 (MQ=255)
                                     aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                                          >  1:3301772/1‑60 (MQ=255)
                                     aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                                          >  1:92915/1‑60 (MQ=255)
                                      agcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaac                              >  1:2726355/1‑70 (MQ=255)
                                      agcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca                                >  1:905232/1‑69 (MQ=255)
                                       gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag                             >  1:829777/1‑71 (MQ=255)
                                         aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCaa                                                             <  1:1002894/37‑1 (MQ=255)
                                         aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                           <  1:1383708/71‑1 (MQ=255)
                                         aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                           <  1:2924668/71‑1 (MQ=255)
                                           cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACTAAGGCCAGCCTGGTCAATCAGc                                         >  1:2284306/1‑55 (MQ=255)
                                           cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGc                                         >  1:480434/1‑55 (MQ=255)
                                           cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg                         <  1:3215487/71‑1 (MQ=255)
                                           cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg                         <  1:3873835/71‑1 (MQ=255)
                                            tGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa                                               <  1:2366504/48‑1 (MQ=255)
                                             ggTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa                                                        >  1:983871/1‑38 (MQ=255)
                                             ggTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa                                                        >  1:399158/1‑38 (MQ=255)
                                              gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa                                                        >  1:2292176/1‑37 (MQ=255)
                                              gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt                        <  1:1244384/69‑1 (MQ=255)
                                              gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt                        <  1:3880562/69‑1 (MQ=255)
                                                tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGcc                                                     >  1:1734052/1‑38 (MQ=255)
                                                tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg                      >  1:2920819/1‑69 (MQ=255)
                                                tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg                      >  1:637619/1‑69 (MQ=255)
                                                   gcgcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt                                  <  1:913837/54‑1 (MQ=255)
                                                   gcgcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTa                 <  1:365271/71‑1 (MQ=255)
                                                    cgcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc                                            >  1:3863507/1‑43 (MQ=255)
                                                     gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca                                      <  1:1557665/48‑1 (MQ=255)
                                                     gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca                                      <  1:2046649/48‑1 (MQ=255)
                                                     gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca                                      <  1:2684378/48‑1 (MQ=255)
                                                     gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAcc               <  1:3127684/71‑1 (MQ=255)
                                                     gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAcc               <  1:2932648/71‑1 (MQ=255)
                                                      ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGtt                       >  1:1222993/1‑62 (MQ=255)
                                                      ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGtt                       >  1:3950220/1‑62 (MQ=255)
                                                       cTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAcc               >  1:867461/1‑69 (MQ=255)
                                                       cTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCa             <  1:844486/71‑1 (MQ=255)
                                                          aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCa                    >  1:3917331/1‑61 (MQ=255)
                                                          aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAg            <  1:3116239/69‑1 (MQ=255)
                                                          aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAg            <  1:534599/69‑1 (MQ=255)
                                                          aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt          >  1:2056128/1‑71 (MQ=255)
                                                          aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt          >  1:3010988/1‑71 (MQ=255)
                                                            tCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTc         >  1:2475754/1‑70 (MQ=255)
                                                             cAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg                      <  1:216579/56‑1 (MQ=255)
                                                              aGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaa                              <  1:2454866/47‑1 (MQ=255)
                                                              aGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca                                >  1:615354/1‑45 (MQ=255)
                                                                tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt                        <  1:1105130/51‑1 (MQ=255)
                                                                  tttCGTGACCAAGGCCCGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGGTGCAGTACCCAGTTCCa       >  1:425849/1‑66 (MQ=255)
                                                                  tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt  >  1:3010677/1‑71 (MQ=255)
                                                                  tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTACACGAtt  >  1:2486565/1‑71 (MQ=255)
                                                                   ttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt  <  1:468957/70‑1 (MQ=255)
                                                                   |                                                                     
CGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATT  >  minE/310727‑310863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: