Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,981 C→T 22.9% P154P (CCG→CCA lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,9810CT22.9% 111.8 / 31.6 61P154P (CCG→CCAlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (21/26);  new base T (9/5);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

GTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGG  >  minE/1933912‑1934051
                                                                     |                                                                      
gTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc                                                                        <  1:2830040/70‑1 (MQ=255)
gTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc                                                                        <  1:1311780/70‑1 (MQ=255)
 ttGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCgg                                                                      <  1:3227969/71‑1 (MQ=255)
     cgatgaggtCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTggta                                                                    >  1:2147575/2‑67 (MQ=255)
      gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg                                                                 >  1:1843248/1‑71 (MQ=255)
          agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg                                                                 >  1:1281842/1‑67 (MQ=255)
          agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg                                                             >  1:2977955/1‑71 (MQ=255)
            atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAg                                                                   <  1:486779/63‑1 (MQ=255)
                agaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGt                                                         >  1:1346340/1‑69 (MQ=255)
                 gaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACgt                                                            >  1:2572066/1‑64 (MQ=255)
                  aTAACGCTGACGATAGCGCGCTTCCTGAGCCTGCAAGCCGTGGAATTTATCCGGc                                                                     <  1:2164308/55‑1 (MQ=255)
                   tAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtt                                                     >  1:1584586/1‑70 (MQ=255)
                     aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta                                                   <  1:3022798/70‑2 (MQ=255)
                     aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta                                                   <  1:3901868/70‑2 (MQ=255)
                     aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCCGCGGACGCAGTGCTTTgg                                                  <  1:1927494/71‑1 (MQ=255)
                     aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg                                                   <  1:3189770/70‑1 (MQ=255)
                      cGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                 >  1:3601980/1‑71 (MQ=255)
                       gCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                 <  1:3547776/70‑1 (MQ=255)
                         tGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                              >  1:119124/1‑71 (MQ=255)
                          gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACgta                                                           >  1:3114064/1‑55 (MQ=255)
                            cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagcag                                           <  1:2671373/71‑1 (MQ=255)
                            cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag                                              >  1:2228930/1‑68 (MQ=255)
                             gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa                                          <  1:1193225/71‑1 (MQ=255)
                               tAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTc                                                >  1:2968468/1‑63 (MQ=255)
                                 gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc                                       <  1:1660476/70‑1 (MQ=255)
                                  cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                 <  1:1574511/59‑1 (MQ=255)
                                  cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa                                     <  1:3551784/71‑1 (MQ=255)
                                  cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa                                     <  1:3120687/71‑1 (MQ=255)
                                     acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTca                                               <  1:1779040/57‑1 (MQ=255)
                                     acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCag                                     >  1:2363474/2‑67 (MQ=255)
                                      cTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg                                 >  1:3761438/1‑71 (MQ=255)
                                        tCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTcag                                >  1:3840113/1‑68 (MQ=255)
                                        tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt                               >  1:909015/1‑71 (MQ=255)
                                         ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                              >  1:3627898/1‑71 (MQ=255)
                                         ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAAGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                              >  1:538962/1‑71 (MQ=255)
                                          cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCgg                                >  1:1742852/1‑68 (MQ=255)
                                          cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt                               >  1:2585507/1‑69 (MQ=255)
                                          cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                             <  1:1109558/71‑1 (MQ=255)
                                            gATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                   <  1:305944/63‑1 (MQ=255)
                                            gATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                   <  1:1777733/63‑1 (MQ=255)
                                             aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACa                            <  1:384925/69‑1 (MQ=255)
                                             aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACa                            <  1:347170/69‑1 (MQ=255)
                                             aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCa                            <  1:1158369/69‑1 (MQ=255)
                                             aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                          >  1:2764946/1‑71 (MQ=255)
                                             aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                          <  1:697177/71‑1 (MQ=255)
                                             aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                          <  1:1279800/71‑1 (MQ=255)
                                              tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag                                              >  1:1865291/1‑50 (MQ=255)
                                                cTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa                       <  1:499918/71‑1 (MQ=255)
                                                     aGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGAc                  <  1:105514/71‑1 (MQ=255)
                                                      gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                   >  1:2930630/1‑69 (MQ=255)
                                                        cGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg                         <  1:1832278/61‑1 (MQ=255)
                                                         gTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGtt              >  1:2548821/1‑71 (MQ=255)
                                                         gTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGtt              >  1:1145555/1‑71 (MQ=255)
                                                          tGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgct              >  1:2314831/1‑68 (MQ=255)
                                                          tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACg                                        >  1:3311014/1‑44 (MQ=255)
                                                          tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg                                 >  1:697659/1‑51 (MQ=255)
                                                          tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa                       >  1:1263051/1‑61 (MQ=255)
                                                          tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACa                 >  1:1297210/1‑67 (MQ=255)
                                                           ggAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                             <  1:788719/54‑1 (MQ=255)
                                                            gAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGc           <  1:2404134/71‑1 (MQ=255)
                                                                ttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCgg        <  1:1235123/70‑1 (MQ=255)
                                                                   tCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt    <  1:1756259/71‑1 (MQ=255)
                                                                   tCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttt     <  1:295452/70‑1 (MQ=255)
                                                                    ccGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttg   >  1:3790899/1‑71 (MQ=255)
                                                                    ccGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt    >  1:1277966/1‑70 (MQ=255)
                                                                     cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg  <  1:3674647/71‑1 (MQ=255)
                                                                     cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg  >  1:1025603/1‑71 (MQ=255)
                                                                     cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg  <  1:3670154/71‑1 (MQ=255)
                                                                     |                                                                      
GTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGG  >  minE/1933912‑1934051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: