Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,993 C→T 14.6% L150L (CTG→CTA lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,9930CT14.6% 185.1 / 21.2 75L150L (CTG→CTAlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (26/38);  new base T (6/5);  total (32/43)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

AGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAG  >  minE/1933928‑1934061
                                                                 |                                                                    
agaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGt                                                                   >  1:1346340/1‑69 (MQ=255)
   tAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtt                                                               >  1:1584586/1‑70 (MQ=255)
     aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta                                                             <  1:3022798/70‑2 (MQ=255)
     aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta                                                             <  1:3901868/70‑2 (MQ=255)
     aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCCGCGGACGCAGTGCTTTgg                                                            <  1:1927494/71‑1 (MQ=255)
     aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg                                                             <  1:3189770/70‑1 (MQ=255)
      cGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                           >  1:3601980/1‑71 (MQ=255)
       gCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                           <  1:3547776/70‑1 (MQ=255)
         tGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                                        >  1:119124/1‑71 (MQ=255)
            cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagcag                                                     <  1:2671373/71‑1 (MQ=255)
            cGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag                                                        >  1:2228930/1‑68 (MQ=255)
             gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa                                                    <  1:1193225/71‑1 (MQ=255)
               tAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTc                                                          >  1:2968468/1‑63 (MQ=255)
                 gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc                                                 <  1:1660476/70‑1 (MQ=255)
                  cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                           <  1:1574511/59‑1 (MQ=255)
                  cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa                                               <  1:3120687/71‑1 (MQ=255)
                  cgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa                                               <  1:3551784/71‑1 (MQ=255)
                     acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTca                                                         <  1:1779040/57‑1 (MQ=255)
                     acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCag                                               >  1:2363474/2‑67 (MQ=255)
                      cTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg                                           >  1:3761438/1‑71 (MQ=255)
                        tCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTcag                                          >  1:3840113/1‑68 (MQ=255)
                        tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt                                         >  1:909015/1‑71 (MQ=255)
                         ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                                        >  1:3627898/1‑71 (MQ=255)
                         ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAAGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                                        >  1:538962/1‑71 (MQ=255)
                          cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCgg                                          >  1:1742852/1‑68 (MQ=255)
                          cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt                                         >  1:2585507/1‑69 (MQ=255)
                          cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                                       <  1:1109558/71‑1 (MQ=255)
                            gATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                             <  1:1777733/63‑1 (MQ=255)
                            gATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                             <  1:305944/63‑1 (MQ=255)
                             aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACa                                      <  1:347170/69‑1 (MQ=255)
                             aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACa                                      <  1:384925/69‑1 (MQ=255)
                             aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCa                                      <  1:1158369/69‑1 (MQ=255)
                             aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                                    <  1:697177/71‑1 (MQ=255)
                             aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                                    >  1:2764946/1‑71 (MQ=255)
                             aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                                    <  1:1279800/71‑1 (MQ=255)
                              tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag                                                        >  1:1865291/1‑50 (MQ=255)
                                cTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa                                 <  1:499918/71‑1 (MQ=255)
                                     aGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGAc                            <  1:105514/71‑1 (MQ=255)
                                      gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                             >  1:2930630/1‑69 (MQ=255)
                                        cGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg                                   <  1:1832278/61‑1 (MQ=255)
                                         gTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGtt                        >  1:2548821/1‑71 (MQ=255)
                                         gTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGtt                        >  1:1145555/1‑71 (MQ=255)
                                          tGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgct                        >  1:2314831/1‑68 (MQ=255)
                                          tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACg                                                  >  1:3311014/1‑44 (MQ=255)
                                          tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg                                           >  1:697659/1‑51 (MQ=255)
                                          tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa                                 >  1:1263051/1‑61 (MQ=255)
                                          tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACa                           >  1:1297210/1‑67 (MQ=255)
                                           ggAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                                       <  1:788719/54‑1 (MQ=255)
                                            gAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGc                     <  1:2404134/71‑1 (MQ=255)
                                                ttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCgg                  <  1:1235123/70‑1 (MQ=255)
                                                   tCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt              <  1:1756259/71‑1 (MQ=255)
                                                   tCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttt               <  1:295452/70‑1 (MQ=255)
                                                    ccGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttg             >  1:3790899/1‑71 (MQ=255)
                                                    ccGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt              >  1:1277966/1‑70 (MQ=255)
                                                     cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg            <  1:3670154/71‑1 (MQ=255)
                                                     cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg            <  1:3674647/71‑1 (MQ=255)
                                                     cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg            >  1:1025603/1‑71 (MQ=255)
                                                      ggCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggt           <  1:676283/71‑1 (MQ=255)
                                                       gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                                       <  1:1318606/42‑1 (MQ=255)
                                                       gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt          <  1:1042513/71‑1 (MQ=255)
                                                       gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt          <  1:1514901/71‑1 (MQ=255)
                                                       gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt          <  1:1181166/71‑1 (MQ=255)
                                                       gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt              <  1:1856460/67‑1 (MQ=255)
                                                          gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg       <  1:3493045/71‑1 (MQ=255)
                                                          gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg       <  1:2485912/71‑1 (MQ=255)
                                                          gCGGACGCAGTGCTTTGGTCAGCAGACGCAACGCGGTGCAg                                     <  1:1690924/41‑1 (MQ=255)
                                                          gCGGACGCAGGGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg       <  1:444897/71‑1 (MQ=255)
                                                           cGGACGCAGTGCTTTGGTCAGCAGGCGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttg             >  1:1449648/1‑64 (MQ=255)
                                                           cGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg       >  1:1871649/1‑70 (MQ=255)
                                                            ggACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc                                       <  1:2371946/37‑1 (MQ=255)
                                                            ggACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtttt        <  1:1396007/68‑1 (MQ=255)
                                                            ggACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGaa     <  1:3493020/71‑1 (MQ=255)
                                                               cGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtttt        >  1:1807359/1‑65 (MQ=255)
                                                               cGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtttt        >  1:1468268/1‑65 (MQ=255)
                                                               cGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAg  >  1:3934171/1‑71 (MQ=255)
                                                                 |                                                                    
AGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAG  >  minE/1933928‑1934061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: