| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,173 | G→A | 9.5% | D90D (GAC→GAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,173 | 0 | G | A | 9.5% | 276.1 / 14.2 | 95 | D90D (GAC→GAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (45/41); new base A (6/3); total (51/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGC > minE/1934104‑1934238 | gAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACg < 1:1929597/70‑1 (MQ=255)gAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGt > 1:753054/1‑71 (MQ=255) aaCTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGt < 1:305061/70‑1 (MQ=255) tgatCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTg > 1:2110839/4‑71 (MQ=255) tGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTg > 1:427440/1‑71 (MQ=255) cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTg < 1:78737/69‑1 (MQ=255) cTCGTTATAAACGCCTTCCGGGAGATCCTCACGGGCAACTTACAGCTGAATGCGACCGCCAACGTCCTg < 1:1628814/69‑1 (MQ=255) tCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa < 1:3229031/70‑1 (MQ=255) tCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATCCTGCaa > 1:3533858/1‑70 (MQ=255) cGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:605132/1‑71 (MQ=255) ttATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt < 1:3138423/71‑1 (MQ=255) tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt > 1:84639/1‑70 (MQ=255) ataAACGCCTTCCGGGAGATTGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa > 1:1146836/1‑71 (MQ=255) aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1251231/71‑1 (MQ=255) aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1110733/71‑1 (MQ=255) aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:414129/71‑1 (MQ=255) aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCt > 1:1558403/1‑58 (MQ=255) aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:2757308/1‑70 (MQ=255) cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:1195468/1‑69 (MQ=255) cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:2351674/1‑69 (MQ=255) cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:3482992/1‑69 (MQ=255) cTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGAc > 1:3822468/1‑71 (MQ=255) tCCGGGAGGTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa > 1:1264911/1‑55 (MQ=255) tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa > 1:1887158/1‑55 (MQ=255) tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa > 1:4883/1‑55 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGTATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc < 1:1339389/70‑1 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg < 1:1693437/69‑1 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:1586997/1‑71 (MQ=255) gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:3897061/61‑1 (MQ=255) gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCtt > 1:2888995/1‑70 (MQ=255) gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:1706607/1‑70 (MQ=255) gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:3931835/1‑70 (MQ=255) gaTCGTCACGGGCAACGTACAGCTGAAAGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:436605/71‑1 (MQ=255) aTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:2413681/70‑1 (MQ=255) aTCGTCACGGGCAACGTACAGCTGAATACGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa > 1:3835260/1‑71 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1893285/57‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:3817657/57‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1682024/57‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:654561/71‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:46477/71‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3872507/71‑1 (MQ=255) cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1667779/1‑70 (MQ=255) cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1019782/1‑70 (MQ=255) cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:2058786/1‑70 (MQ=255) gTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc > 1:1479895/1‑67 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg < 1:2661970/46‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:2197201/52‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2383178/1‑70 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:874430/1‑70 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa > 1:3636070/1‑52 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa > 1:3682362/1‑52 (MQ=255) aCGTGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:1721183/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTa > 1:801775/1‑46 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa > 1:314147/1‑52 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:3568748/71‑1 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:2946840/71‑1 (MQ=255) cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:1342083/4‑70 (MQ=255) gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCata > 1:2664461/1‑65 (MQ=255) gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATTATacga < 1:3212337/71‑1 (MQ=255) gCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAAc > 1:373295/1‑44 (MQ=255) gCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg < 1:1001202/71‑1 (MQ=255) cAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1176573/47‑1 (MQ=255) cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtc < 1:3945102/71‑1 (MQ=255) cGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:2175408/1‑50 (MQ=255) aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc > 1:1685103/1‑46 (MQ=255) aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2562071/1‑70 (MQ=255) aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:3091605/1‑70 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1020139/70‑1 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1719053/70‑1 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:146099/70‑1 (MQ=255) aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCTATAATACGACGGGTcatcat < 1:1842042/71‑1 (MQ=255) ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:1474238/2‑69 (MQ=255) ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:2098882/2‑69 (MQ=255) ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcat < 1:3442194/68‑1 (MQ=255) cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACggg < 1:2773660/62‑1 (MQ=255) cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3424822/70‑1 (MQ=255) cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3422759/70‑1 (MQ=255) tGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:2419705/58‑1 (MQ=255) gAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg > 1:613890/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2435959/1‑71 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg < 1:1966525/70‑1 (MQ=255) aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:1549006/70‑1 (MQ=255) aTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc < 1:3845564/70‑1 (MQ=255) aCCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa < 1:1676818/71‑1 (MQ=255) cGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg < 1:3731146/71‑1 (MQ=255) gcaaaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg > 1:1476048/4‑71 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAAAgg > 1:1291757/1‑71 (MQ=255) gCCAACGTCCTGCA‑GGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:1437688/71‑1 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata < 1:3426055/42‑1 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:2043395/71‑1 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc > 1:3403473/1‑71 (MQ=255) aaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCa > 1:1720525/1‑61 (MQ=255) aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata > 1:1275403/1‑39 (MQ=255) aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:2494688/1‑55 (MQ=255) aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:3071995/1‑55 (MQ=255) aCGTCCTGCAGGGTAACGAAAGACGATTTACCCataata > 1:879284/1‑39 (MQ=255) | GAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGC > minE/1934104‑1934238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |