| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,188 | G→A | 11.2% | F85F (TTC→TTT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,188 | 0 | G | A | 11.2% | 271.1 / 18.8 | 98 | F85F (TTC→TTT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (42/45); new base A (5/6); total (47/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
AACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCT > minE/1934119‑1934257 | aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:414129/71‑1 (MQ=255)aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1110733/71‑1 (MQ=255)aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1251231/71‑1 (MQ=255) aCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:2757308/1‑70 (MQ=255) cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:3482992/1‑69 (MQ=255) cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:2351674/1‑69 (MQ=255) cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa > 1:1195468/1‑69 (MQ=255) cTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGAc > 1:3822468/1‑71 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGTATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc < 1:1339389/70‑1 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg < 1:1693437/69‑1 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:1586997/1‑71 (MQ=255) gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:3897061/61‑1 (MQ=255) gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCtt > 1:2888995/1‑70 (MQ=255) gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:3931835/1‑70 (MQ=255) gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAcc > 1:1706607/1‑70 (MQ=255) gaTCGTCACGGGCAACGTACAGCTGAAAGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:436605/71‑1 (MQ=255) aTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:2413681/70‑1 (MQ=255) aTCGTCACGGGCAACGTACAGCTGAATACGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa > 1:3835260/1‑71 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:3817657/57‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1893285/57‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1682024/57‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:654561/71‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:46477/71‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3872507/71‑1 (MQ=255) cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1019782/1‑70 (MQ=255) cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:2058786/1‑70 (MQ=255) cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1667779/1‑70 (MQ=255) gTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc > 1:1479895/1‑67 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:2197201/52‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2383178/1‑70 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:874430/1‑70 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa > 1:3636070/1‑52 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa > 1:3682362/1‑52 (MQ=255) aCGTGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:1721183/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa > 1:314147/1‑52 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:3568748/71‑1 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:2946840/71‑1 (MQ=255) cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:1342083/4‑70 (MQ=255) gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCata > 1:2664461/1‑65 (MQ=255) gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATTATacga < 1:3212337/71‑1 (MQ=255) gCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg < 1:1001202/71‑1 (MQ=255) cAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:1176573/47‑1 (MQ=255) cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtc < 1:3945102/71‑1 (MQ=255) cGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:2175408/1‑50 (MQ=255) aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc > 1:1685103/1‑46 (MQ=255) aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:3091605/1‑70 (MQ=255) aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2562071/1‑70 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1719053/70‑1 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:146099/70‑1 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:1020139/70‑1 (MQ=255) aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCTATAATACGACGGGTcatcat < 1:1842042/71‑1 (MQ=255) ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:1474238/2‑69 (MQ=255) ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcattc > 1:2098882/2‑69 (MQ=255) ttGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTcatcat < 1:3442194/68‑1 (MQ=255) cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACggg < 1:2773660/62‑1 (MQ=255) cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3424822/70‑1 (MQ=255) cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3422759/70‑1 (MQ=255) tGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:2419705/58‑1 (MQ=255) gAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg > 1:613890/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2435959/1‑71 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg < 1:1966525/70‑1 (MQ=255) aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:1549006/70‑1 (MQ=255) aTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc < 1:3845564/70‑1 (MQ=255) aCCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa < 1:1676818/71‑1 (MQ=255) cGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg < 1:3731146/71‑1 (MQ=255) gcaaaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg > 1:1476048/4‑71 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAAAgg > 1:1291757/1‑71 (MQ=255) gCCAACGTCCTGCA‑GGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:1437688/71‑1 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata < 1:3426055/42‑1 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:2043395/71‑1 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc > 1:3403473/1‑71 (MQ=255) aaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCa > 1:1720525/1‑61 (MQ=255) aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata > 1:1275403/1‑39 (MQ=255) aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:2494688/1‑55 (MQ=255) aCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:3071995/1‑55 (MQ=255) aCGTCCTGCAGGGTAACGAAAGACGATTTACCCataata > 1:879284/1‑39 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2141904/71‑1 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2322979/71‑1 (MQ=255) tCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTc < 1:2844694/71‑1 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:1274658/70‑1 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:2069430/70‑1 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa < 1:2120878/71‑1 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:3842180/70‑2 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:1776962/70‑2 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACAGAGACTTcaa > 1:1040718/1‑69 (MQ=255) tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACtt < 1:1167715/67‑1 (MQ=255) cAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat > 1:327039/1‑45 (MQ=255) aGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:258544/1‑71 (MQ=255) aGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:598419/1‑71 (MQ=255) ggTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa < 1:3160277/71‑1 (MQ=255) tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:1337919/62‑1 (MQ=255) aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg < 1:78977/52‑1 (MQ=255) aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg > 1:3533358/1‑71 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGAt > 1:3665650/1‑62 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:696461/1‑64 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa > 1:3529402/1‑66 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt < 1:3849325/71‑1 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt > 1:3248566/1‑71 (MQ=255) | AACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCT > minE/1934119‑1934257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |