| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,238 | C→A | 19.4% | A69S (GCC→TCC) ‡ | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,238 | 0 | C | A | 19.4% | 202.1 / 26.7 | 77 | A69S (GCC→TCC) ‡ | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (29/33); new base A (4/11); total (33/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
GCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTC > minE/1934167‑1934304 | gCCAACGTCCTGCA‑GGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:1437688/71‑1 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc > 1:3403473/1‑71 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:2043395/71‑1 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2322979/71‑1 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2141904/71‑1 (MQ=255) tCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTc < 1:2844694/71‑1 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:1274658/70‑1 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:2069430/70‑1 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa < 1:2120878/71‑1 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:3842180/70‑2 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:1776962/70‑2 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACAGAGACTTcaa > 1:1040718/1‑69 (MQ=255) tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACtt < 1:1167715/67‑1 (MQ=255) aGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:598419/1‑71 (MQ=255) aGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:258544/1‑71 (MQ=255) ggTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa < 1:3160277/71‑1 (MQ=255) tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg < 1:1337919/62‑1 (MQ=255) aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg > 1:3533358/1‑71 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGAt > 1:3665650/1‑62 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:696461/1‑64 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa > 1:3529402/1‑66 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt > 1:3248566/1‑71 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt < 1:3849325/71‑1 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGAc > 1:1816200/1‑52 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc > 1:66336/1‑70 (MQ=255) gaggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAg > 1:2529101/4‑71 (MQ=255) gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc < 1:3302059/71‑1 (MQ=255) gCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttc < 1:3534421/71‑1 (MQ=255) cTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttct < 1:3938307/71‑1 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGAt > 1:2104523/1‑50 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGAt > 1:2433148/1‑50 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc > 1:3348784/1‑71 (MQ=255) ccATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt < 1:3335348/66‑1 (MQ=255) cATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTcaa > 1:2869328/1‑42 (MQ=255) cATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTcaa > 1:1452122/1‑42 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2128789/42‑1 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc < 1:1460137/66‑1 (MQ=255) tgataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTtatcc < 1:2818886/69‑2 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct < 1:425590/71‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct < 1:2329692/71‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct < 1:170121/71‑1 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:37839/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:3586116/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:3785940/1‑71 (MQ=255) ataCGACGGGTCATCATTCGGCCAGCAACCGAGCCTTCAATGTTTAAGGATTCCAGTTCCTGGTtatcct < 1:860711/70‑3 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa > 1:372350/1‑46 (MQ=255) taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt < 1:2698314/70‑1 (MQ=255) taCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt < 1:190178/69‑1 (MQ=255) acgacgGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcct < 1:27264/59‑3 (MQ=255) acgacgGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcct < 1:461225/59‑3 (MQ=255) gacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc < 1:2382506/50‑1 (MQ=255) acgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt < 1:760319/65‑1 (MQ=255) acgGGTCATCATGCGGCCAGCAACGGAGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt < 1:2024128/65‑1 (MQ=255) cgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:3508625/1‑65 (MQ=255) gggTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg < 1:3623730/71‑1 (MQ=255) gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa < 1:1904250/71‑1 (MQ=255) tcatcaTGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc > 1:509796/1‑58 (MQ=255) tcatcaTGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt > 1:2079312/1‑71 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGt > 1:3870703/1‑53 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt > 1:2449958/1‑70 (MQ=255) atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc < 1:1635228/47‑1 (MQ=255) atcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct < 1:2672363/57‑1 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:1086959/1‑57 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:3895473/70‑1 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg > 1:1004369/1‑71 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc > 1:3580720/1‑71 (MQ=255) tGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc < 1:475559/38‑1 (MQ=255) gCCAGCAACGGCGACTTCTATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCa < 1:3632756/71‑1 (MQ=255) gCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:430752/1‑60 (MQ=255) ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt < 1:590094/60‑1 (MQ=255) cAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAt > 1:797984/1‑71 (MQ=255) aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt > 1:989762/1‑59 (MQ=255) aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAtt > 1:56651/1‑71 (MQ=255) aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAtt < 1:2212748/71‑1 (MQ=255) gCAACGGCGACTTCGATGTTCAGTGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg < 1:3438780/71‑1 (MQ=255) gCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg < 1:994345/71‑1 (MQ=255) cAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:1360918/1‑55 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCa < 1:3577816/64‑1 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg < 1:2617371/68‑1 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTc < 1:2290633/71‑1 (MQ=255) | GCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTC > minE/1934167‑1934304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |