| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,268 | C→G | 10.4% | E59Q (GAA→CAA) ‡ | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,268 | 0 | C | G | 10.4% | 211.0 / 13.0 | 77 | E59Q (GAA→CAA) ‡ | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (38/31); new base G (3/5); total (41/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
TTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAA > minE/1934198‑1934337 | ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc > 1:3348784/1‑71 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc < 1:1460137/66‑1 (MQ=255) tgataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTtatcc < 1:2818886/69‑2 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct < 1:2329692/71‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct < 1:170121/71‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct < 1:425590/71‑1 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:3586116/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:3785940/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:37839/1‑71 (MQ=255) ataCGACGGGTCATCATTCGGCCAGCAACCGAGCCTTCAATGTTTAAGGATTCCAGTTCCTGGTtatcct < 1:860711/70‑3 (MQ=255) taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt < 1:2698314/70‑1 (MQ=255) taCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt < 1:190178/69‑1 (MQ=255) acgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt < 1:760319/65‑1 (MQ=255) acgGGTCATCATGCGGCCAGCAACGGAGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt < 1:2024128/65‑1 (MQ=255) cgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:3508625/1‑65 (MQ=255) gggTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg < 1:3623730/71‑1 (MQ=255) gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa < 1:1904250/71‑1 (MQ=255) tcatcaTGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc > 1:509796/1‑58 (MQ=255) tcatcaTGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt > 1:2079312/1‑71 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGt > 1:3870703/1‑53 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt > 1:2449958/1‑70 (MQ=255) atcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct < 1:2672363/57‑1 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:1086959/1‑57 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:3895473/70‑1 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg > 1:1004369/1‑71 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc > 1:3580720/1‑71 (MQ=255) gCCAGCAACGGCGACTTCTATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCa < 1:3632756/71‑1 (MQ=255) gCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:430752/1‑60 (MQ=255) ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt < 1:590094/60‑1 (MQ=255) cAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAt > 1:797984/1‑71 (MQ=255) aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt > 1:989762/1‑59 (MQ=255) aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAtt > 1:56651/1‑71 (MQ=255) aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAtt < 1:2212748/71‑1 (MQ=255) gCAACGGCGACTTCGATGTTCAGTGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg < 1:3438780/71‑1 (MQ=255) gCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg < 1:994345/71‑1 (MQ=255) cAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:1360918/1‑55 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCa < 1:3577816/64‑1 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg < 1:2617371/68‑1 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTc < 1:2290633/71‑1 (MQ=255) gACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGcc < 1:238993/42‑1 (MQ=255) aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAgg < 1:1001321/70‑1 (MQ=255) cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGt > 1:413024/1‑42 (MQ=255) cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt > 1:1893917/1‑54 (MQ=255) cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt > 1:3077641/1‑54 (MQ=255) cTTCGATGTTCAGCGCTTACAGTTCTTCGTTCTCTTTGCCg > 1:2315693/1‑41 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt < 1:1798553/44‑1 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTg > 1:1663257/1‑52 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt > 1:3374137/1‑60 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTAt > 1:2489960/1‑69 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATg < 1:19321/70‑1 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:2930972/1‑71 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:874366/1‑71 (MQ=255) gATGTTCAGCGCTTCCAGTTCTTCGGTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa < 1:1010110/70‑1 (MQ=255) aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCaga > 1:1201739/1‑62 (MQ=255) tGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:3409677/1‑40 (MQ=255) ttCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTc < 1:2001492/41‑1 (MQ=255) tCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg < 1:810349/35‑1 (MQ=255) tCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc > 1:177321/1‑69 (MQ=255) cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt > 1:2138374/1‑38 (MQ=255) cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc > 1:3419140/1‑71 (MQ=255) cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc > 1:3699312/1‑71 (MQ=255) cAGAGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt > 1:1711027/1‑38 (MQ=255) aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACg > 1:2465454/1‑71 (MQ=255) gcgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACgg < 1:249032/71‑1 (MQ=255) cgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGa < 1:640171/71‑1 (MQ=255) gcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGaa < 1:95399/71‑1 (MQ=255) gattCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGaaa < 1:3352812/69‑1 (MQ=255) cTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTTGTCAGAGGTATGATCGCGACGGaa < 1:1306342/70‑1 (MQ=255) ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGaaaa > 1:3717359/1‑70 (MQ=255) ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt > 1:1094387/1‑71 (MQ=255) tCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATc > 1:1628646/1‑71 (MQ=255) tCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt > 1:1441068/1‑70 (MQ=255) ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAAt < 1:914869/69‑1 (MQ=255) cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGa > 1:135864/1‑61 (MQ=255) cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg < 1:277073/70‑1 (MQ=255) cAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATca < 1:2108073/70‑2 (MQ=255) cAGTTATTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg > 1:1832902/1‑42 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt > 1:1236295/1‑71 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt > 1:2157153/1‑71 (MQ=255) ttcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCg < 1:3722214/71‑1 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCa < 1:514162/41‑1 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCa < 1:3893408/41‑1 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg > 1:1519415/1‑70 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg > 1:1925090/1‑70 (MQ=255) ttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCagag < 1:1117770/43‑1 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa < 1:2298148/70‑1 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa < 1:2734720/70‑1 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGa < 1:2677430/69‑1 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGa < 1:161816/69‑1 (MQ=255) | TTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAA > minE/1934198‑1934337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |