Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,934,287 T→C 6.6% E52E (GAA→GAG lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,934,2870TC 6.6% 213.9 / 5.1 76E52E (GAA→GAGlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (31/40);  new base C (3/2);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

TCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCA  >  minE/1934217‑1934357
                                                                      |                                                                      
tcatcaTGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt                                                                        >  1:2079312/1‑71 (MQ=255)
 catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt                                                                        >  1:2449958/1‑70 (MQ=255)
    catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt                                                                     <  1:3895473/70‑1 (MQ=255)
    catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg                                                                    >  1:1004369/1‑71 (MQ=255)
     atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc                                                                   >  1:3580720/1‑71 (MQ=255)
          gCCAGCAACGGCGACTTCTATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCa                                                              <  1:3632756/71‑1 (MQ=255)
           ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt                                                                        <  1:590094/60‑1 (MQ=255)
            cAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAt                                                            >  1:797984/1‑71 (MQ=255)
             aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt                                                                       >  1:989762/1‑59 (MQ=255)
             aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAtt                                                           >  1:56651/1‑71 (MQ=255)
             aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAtt                                                           <  1:2212748/71‑1 (MQ=255)
              gCAACGGCGACTTCGATGTTCAGTGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg                                                          <  1:3438780/71‑1 (MQ=255)
              gCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg                                                          <  1:994345/71‑1 (MQ=255)
                 aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCa                                                              <  1:3577816/64‑1 (MQ=255)
                 aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg                                                          <  1:2617371/68‑1 (MQ=255)
                 aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTc                                                       <  1:2290633/71‑1 (MQ=255)
                       aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAgg                                                  <  1:1001321/70‑1 (MQ=255)
                        cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt                                                                 >  1:3077641/1‑54 (MQ=255)
                        cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt                                                                 >  1:1893917/1‑54 (MQ=255)
                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt                                                                        <  1:1798553/44‑1 (MQ=255)
                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTg                                                                >  1:1663257/1‑52 (MQ=255)
                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt                                                        >  1:3374137/1‑60 (MQ=255)
                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTAt                                               >  1:2489960/1‑69 (MQ=255)
                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATg                                              <  1:19321/70‑1 (MQ=255)
                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa                                             >  1:2930972/1‑71 (MQ=255)
                           cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa                                             >  1:874366/1‑71 (MQ=255)
                            gATGTTCAGCGCTTCCAGTTCTTCGGTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa                                             <  1:1010110/70‑1 (MQ=255)
                             aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCaga                                                    >  1:1201739/1‑62 (MQ=255)
                                ttCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTc                                                                      <  1:2001492/41‑1 (MQ=255)
                                 tCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc                                         >  1:177321/1‑69 (MQ=255)
                                  cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt                                                                       >  1:2138374/1‑38 (MQ=255)
                                  cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc                                      >  1:3419140/1‑71 (MQ=255)
                                  cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc                                      >  1:3699312/1‑71 (MQ=255)
                                  cAGAGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt                                                                       >  1:1711027/1‑38 (MQ=255)
                                   aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACg                                     >  1:2465454/1‑71 (MQ=255)
                                    gcgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACgg                                    <  1:249032/71‑1 (MQ=255)
                                     cgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGa                                   <  1:640171/71‑1 (MQ=255)
                                      gcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGaa                                  <  1:95399/71‑1 (MQ=255)
                                      gattCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGaaa                                  <  1:3352812/69‑1 (MQ=255)
                                       cTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTTGTCAGAGGTATGATCGCGACGGaa                                  <  1:1306342/70‑1 (MQ=255)
                                        ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGaaaa                                 >  1:3717359/1‑70 (MQ=255)
                                        ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt                                >  1:1094387/1‑71 (MQ=255)
                                         tCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATc                               >  1:1628646/1‑71 (MQ=255)
                                         tCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt                                >  1:1441068/1‑70 (MQ=255)
                                          ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAAt                                <  1:914869/69‑1 (MQ=255)
                                           cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGa                                       >  1:135864/1‑61 (MQ=255)
                                           cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg                              <  1:277073/70‑1 (MQ=255)
                                           cAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATca                              <  1:2108073/70‑2 (MQ=255)
                                           cAGTTATTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg                                                          >  1:1832902/1‑42 (MQ=255)
                                            aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt                            >  1:1236295/1‑71 (MQ=255)
                                            aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt                            >  1:2157153/1‑71 (MQ=255)
                                              ttcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCg                          <  1:3722214/71‑1 (MQ=255)
                                                cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCa                                                      <  1:3893408/41‑1 (MQ=255)
                                                cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCa                                                      <  1:514162/41‑1 (MQ=255)
                                                cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg                         >  1:1519415/1‑70 (MQ=255)
                                                cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg                         >  1:1925090/1‑70 (MQ=255)
                                                 ttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCagag                                                   <  1:1117770/43‑1 (MQ=255)
                                                   cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa                      <  1:2298148/70‑1 (MQ=255)
                                                   cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa                      <  1:2734720/70‑1 (MQ=255)
                                                   cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGa                       <  1:161816/69‑1 (MQ=255)
                                                   cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGa                       <  1:2677430/69‑1 (MQ=255)
                                                    gTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc                                         >  1:1207895/1‑50 (MQ=255)
                                                    gTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAgg                    <  1:2219731/71‑1 (MQ=255)
                                                     ttCTCTTTGCCGTCGAATTCTGCGTGCATTTGGTCAGAGGTATGa                                             <  1:2630468/45‑1 (MQ=255)
                                                     ttCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa                                             <  1:3478289/45‑1 (MQ=255)
                                                     ttCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGc                   <  1:699005/71‑1 (MQ=255)
                                                       ctctTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa                 >  1:3139375/1‑71 (MQ=255)
                                                       ctctTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa                 >  1:3811096/1‑71 (MQ=255)
                                                       atctTTGACGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGAt                                            >  1:2815884/2‑44 (MQ=255)
                                                          tttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa                 <  1:2659247/68‑1 (MQ=255)
                                                          tttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAAt                <  1:1168111/69‑1 (MQ=255)
                                                           ttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCa                  >  1:1427207/1‑66 (MQ=255)
                                                            tGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATcc              <  1:1442494/69‑1 (MQ=255)
                                                            tGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATcc              <  1:2087645/69‑1 (MQ=255)
                                                                  cGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcg      >  1:1531808/1‑71 (MQ=255)
                                                                   gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa                 >  1:2011953/1‑59 (MQ=255)
                                                                   gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgc     <  1:2488411/71‑1 (MQ=255)
                                                                   gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgc     <  1:3377989/71‑1 (MQ=255)
                                                                   gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcg      <  1:3494579/70‑1 (MQ=255)
                                                                    aaTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGa                                   <  1:364758/40‑1 (MQ=255)
                                                                     aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg    >  1:2798313/1‑70 (MQ=255)
                                                                     aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCgcgc   <  1:2632238/71‑1 (MQ=255)
                                                                     aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCgcgc   <  1:1695486/71‑1 (MQ=255)
                                                                      ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa                      >  1:2455871/1‑51 (MQ=255)
                                                                      ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg    >  1:2041155/1‑69 (MQ=255)
                                                                      ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCa  <  1:3076383/71‑1 (MQ=255)
                                                                      |                                                                      
TCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCA  >  minE/1934217‑1934357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: