Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,934,291 G→T 8.7% A51E (GCA→GAA)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,934,2910GT 8.7% 259.7 / 6.7 80A51E (GCA→GAA) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (34/39);  new base T (4/3);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

CATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGG  >  minE/1934221‑1934359
                                                                      |                                                                    
catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg                                                                      >  1:1004369/1‑71 (MQ=255)
 atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc                                                                     >  1:3580720/1‑71 (MQ=255)
      gCCAGCAACGGCGACTTCTATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCa                                                                <  1:3632756/71‑1 (MQ=255)
        cAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAt                                                              >  1:797984/1‑71 (MQ=255)
         aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAtt                                                             >  1:56651/1‑71 (MQ=255)
         aGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAAtt                                                             <  1:2212748/71‑1 (MQ=255)
          gCAACGGCGACTTCGATGTTCAGTGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg                                                            <  1:3438780/71‑1 (MQ=255)
          gCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg                                                            <  1:994345/71‑1 (MQ=255)
             aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCa                                                                <  1:3577816/64‑1 (MQ=255)
             aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg                                                            <  1:2617371/68‑1 (MQ=255)
             aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTc                                                         <  1:2290633/71‑1 (MQ=255)
                   aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAgg                                                    <  1:1001321/70‑1 (MQ=255)
                    cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt                                                                   >  1:1893917/1‑54 (MQ=255)
                    cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt                                                                   >  1:3077641/1‑54 (MQ=255)
                       cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTg                                                                  >  1:1663257/1‑52 (MQ=255)
                       cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt                                                          >  1:3374137/1‑60 (MQ=255)
                       cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTAt                                                 >  1:2489960/1‑69 (MQ=255)
                       cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATg                                                <  1:19321/70‑1 (MQ=255)
                       cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa                                               >  1:874366/1‑71 (MQ=255)
                       cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa                                               >  1:2930972/1‑71 (MQ=255)
                        gATGTTCAGCGCTTCCAGTTCTTCGGTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa                                               <  1:1010110/70‑1 (MQ=255)
                         aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCaga                                                      >  1:1201739/1‑62 (MQ=255)
                             tCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc                                           >  1:177321/1‑69 (MQ=255)
                              cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc                                        >  1:3699312/1‑71 (MQ=255)
                              cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc                                        >  1:3419140/1‑71 (MQ=255)
                               aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACg                                       >  1:2465454/1‑71 (MQ=255)
                                gcgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACgg                                      <  1:249032/71‑1 (MQ=255)
                                 cgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGa                                     <  1:640171/71‑1 (MQ=255)
                                  gcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGaa                                    <  1:95399/71‑1 (MQ=255)
                                  gattCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGaaa                                    <  1:3352812/69‑1 (MQ=255)
                                   cTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTTGTCAGAGGTATGATCGCGACGGaa                                    <  1:1306342/70‑1 (MQ=255)
                                    ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGaaaa                                   >  1:3717359/1‑70 (MQ=255)
                                    ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt                                  >  1:1094387/1‑71 (MQ=255)
                                     tCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATc                                 >  1:1628646/1‑71 (MQ=255)
                                     tCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt                                  >  1:1441068/1‑70 (MQ=255)
                                      ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAAt                                  <  1:914869/69‑1 (MQ=255)
                                       cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGa                                         >  1:135864/1‑61 (MQ=255)
                                       cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg                                <  1:277073/70‑1 (MQ=255)
                                       cAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATca                                <  1:2108073/70‑2 (MQ=255)
                                       cAGTTATTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg                                                            >  1:1832902/1‑42 (MQ=255)
                                        aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt                              >  1:2157153/1‑71 (MQ=255)
                                        aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt                              >  1:1236295/1‑71 (MQ=255)
                                          ttcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCg                            <  1:3722214/71‑1 (MQ=255)
                                            cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCa                                                        <  1:3893408/41‑1 (MQ=255)
                                            cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCa                                                        <  1:514162/41‑1 (MQ=255)
                                            cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg                           >  1:1519415/1‑70 (MQ=255)
                                            cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg                           >  1:1925090/1‑70 (MQ=255)
                                             ttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCagag                                                     <  1:1117770/43‑1 (MQ=255)
                                               cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa                        <  1:2734720/70‑1 (MQ=255)
                                               cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa                        <  1:2298148/70‑1 (MQ=255)
                                               cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGa                         <  1:2677430/69‑1 (MQ=255)
                                               cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGa                         <  1:161816/69‑1 (MQ=255)
                                                gTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc                                           >  1:1207895/1‑50 (MQ=255)
                                                gTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAgg                      <  1:2219731/71‑1 (MQ=255)
                                                 ttCTCTTTGCCGTCGAATTCTGCGTGCATTTGGTCAGAGGTATGa                                               <  1:2630468/45‑1 (MQ=255)
                                                 ttCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa                                               <  1:3478289/45‑1 (MQ=255)
                                                 ttCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGc                     <  1:699005/71‑1 (MQ=255)
                                                   ctctTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa                   >  1:3139375/1‑71 (MQ=255)
                                                   ctctTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa                   >  1:3811096/1‑71 (MQ=255)
                                                   atctTTGACGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGAt                                              >  1:2815884/2‑44 (MQ=255)
                                                      tttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa                   <  1:2659247/68‑1 (MQ=255)
                                                      tttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAAt                  <  1:1168111/69‑1 (MQ=255)
                                                       ttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCa                    >  1:1427207/1‑66 (MQ=255)
                                                        tGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATcc                <  1:2087645/69‑1 (MQ=255)
                                                        tGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATcc                <  1:1442494/69‑1 (MQ=255)
                                                              cGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcg        >  1:1531808/1‑71 (MQ=255)
                                                               gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa                   >  1:2011953/1‑59 (MQ=255)
                                                               gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgc       <  1:2488411/71‑1 (MQ=255)
                                                               gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgc       <  1:3377989/71‑1 (MQ=255)
                                                               gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcg        <  1:3494579/70‑1 (MQ=255)
                                                                aaTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGa                                     <  1:364758/40‑1 (MQ=255)
                                                                 aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg      >  1:2798313/1‑70 (MQ=255)
                                                                 aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCgcgc     <  1:1695486/71‑1 (MQ=255)
                                                                 aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCgcgc     <  1:2632238/71‑1 (MQ=255)
                                                                  ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa                        >  1:2455871/1‑51 (MQ=255)
                                                                  ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg      >  1:2041155/1‑69 (MQ=255)
                                                                  ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCa    <  1:3076383/71‑1 (MQ=255)
                                                                   tCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATCAttgg                            <  1:6512/46‑3 (MQ=16)
                                                                   tCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATCAtt                              >  1:2034962/1‑44 (MQ=16)
                                                                    cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt                              >  1:390649/1‑43 (MQ=255)
                                                                    cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAgg  >  1:2717760/1‑71 (MQ=255)
                                                                      |                                                                    
CATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGG  >  minE/1934221‑1934359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: