| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,320 | A→G | 7.8% | R41R (CGT→CGC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,320 | 0 | A | G | 7.8% | 247.9 / 9.8 | 89 | R41R (CGT→CGC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (44/38); new base G (4/3); total (48/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01 | |||||||||||
CAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGT > minE/1934251‑1934389 | cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc > 1:3699312/1‑71 (MQ=255)cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc > 1:3419140/1‑71 (MQ=255) aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACg > 1:2465454/1‑71 (MQ=255) gcgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACgg < 1:249032/71‑1 (MQ=255) cgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGa < 1:640171/71‑1 (MQ=255) gcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGaa < 1:95399/71‑1 (MQ=255) gattCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGaaa < 1:3352812/69‑1 (MQ=255) cTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTTGTCAGAGGTATGATCGCGACGGaa < 1:1306342/70‑1 (MQ=255) ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGaaaa > 1:3717359/1‑70 (MQ=255) ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt > 1:1094387/1‑71 (MQ=255) tCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATc > 1:1628646/1‑71 (MQ=255) tCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt > 1:1441068/1‑70 (MQ=255) ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAAt < 1:914869/69‑1 (MQ=255) cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGa > 1:135864/1‑61 (MQ=255) cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg < 1:277073/70‑1 (MQ=255) cAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATca < 1:2108073/70‑2 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt > 1:2157153/1‑71 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt > 1:1236295/1‑71 (MQ=255) ttcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCg < 1:3722214/71‑1 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg > 1:1925090/1‑70 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg > 1:1519415/1‑70 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa < 1:2734720/70‑1 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa < 1:2298148/70‑1 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGa < 1:161816/69‑1 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGa < 1:2677430/69‑1 (MQ=255) gTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAgg < 1:2219731/71‑1 (MQ=255) ttCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGc < 1:699005/71‑1 (MQ=255) ctctTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa > 1:3811096/1‑71 (MQ=255) ctctTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa > 1:3139375/1‑71 (MQ=255) tttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa < 1:2659247/68‑1 (MQ=255) tttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAAt < 1:1168111/69‑1 (MQ=255) ttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCa > 1:1427207/1‑66 (MQ=255) tGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATcc < 1:1442494/69‑1 (MQ=255) tGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATcc < 1:2087645/69‑1 (MQ=255) cGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcg > 1:1531808/1‑71 (MQ=255) gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa > 1:2011953/1‑59 (MQ=255) gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgc < 1:2488411/71‑1 (MQ=255) gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgc < 1:3377989/71‑1 (MQ=255) gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcg < 1:3494579/70‑1 (MQ=255) aaTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGa < 1:364758/40‑1 (MQ=255) aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg > 1:2798313/1‑70 (MQ=255) aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCgcgc < 1:2632238/71‑1 (MQ=255) aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCgcgc < 1:1695486/71‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa > 1:2455871/1‑51 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg > 1:2041155/1‑69 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCa < 1:3076383/71‑1 (MQ=255) tCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATCAttgg < 1:6512/46‑3 (MQ=16) tCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATCAtt > 1:2034962/1‑44 (MQ=16) cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt > 1:390649/1‑43 (MQ=255) cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAgg > 1:2717760/1‑71 (MQ=255) cGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGt < 1:2388826/69‑1 (MQ=255) cGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTc < 1:3018823/71‑1 (MQ=255) cGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTc < 1:3751951/71‑1 (MQ=255) cGTGCAATGGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTc > 1:38206/1‑71 (MQ=255) gTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCg < 1:3372527/71‑1 (MQ=255) gTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCg < 1:3192608/71‑1 (MQ=255) gCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGc > 1:3658725/1‑70 (MQ=255) cAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg > 1:3890924/1‑39 (MQ=255) cAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg > 1:943347/1‑39 (MQ=255) cAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg > 1:3449737/1‑39 (MQ=255) cAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg > 1:3060033/1‑39 (MQ=255) cAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCa > 1:1120957/1‑46 (MQ=255) cAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCa < 1:3539421/71‑1 (MQ=255) ttGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATc < 1:488857/46‑1 (MQ=255) tGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCtt > 1:468530/1‑71 (MQ=255) ggTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTtc > 1:1695775/1‑71 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAg > 1:371338/1‑54 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAg > 1:1636447/1‑54 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAg > 1:2959797/1‑54 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCac > 1:618605/1‑71 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCac > 1:3586517/1‑71 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCac < 1:3822475/71‑1 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCac > 1:3331198/1‑71 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCa > 1:2733781/1‑70 (MQ=255) gagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCac > 1:3076082/1‑70 (MQ=255) ggTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGt < 1:1087381/53‑1 (MQ=255) tATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGc < 1:459906/59‑1 (MQ=255) tATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGc < 1:1244526/59‑1 (MQ=255) aTGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGAcgcg > 1:3908423/1‑71 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAg < 1:1427527/54‑1 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGttt > 1:3563643/1‑71 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGtt < 1:487502/70‑1 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCGCGAGCAgg > 1:2019470/1‑46 (MQ=255) tCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGtttt > 1:26038/1‑71 (MQ=255) cgcgACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGtttt > 1:70609/1‑70 (MQ=255) gcgACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGtttt > 1:229987/1‑69 (MQ=255) gcgACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGtttt > 1:764264/1‑69 (MQ=255) gcgACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTc < 1:647699/70‑1 (MQ=255) gACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCt > 1:2543128/1‑51 (MQ=255) gACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGt > 1:2031295/1‑71 (MQ=255) | CAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTTTCAGT > minE/1934251‑1934389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |