| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 310,821 | C→T | 9.3% | L684L (CTG→CTA) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 310,821 | 0 | C | T | 9.3% | 249.8 / 11.3 | 86 | L684L (CTG→CTA) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (36/42); new base T (4/4); total (40/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATC > minE/310752‑310890 | cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc < 1:2051395/70‑1 (MQ=255) gctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg < 1:646128/71‑1 (MQ=255) gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt > 1:3763047/1‑70 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt < 1:471647/69‑1 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt < 1:901874/69‑1 (MQ=255) tCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc > 1:1954965/1‑68 (MQ=255) tGCAAGCAACTTGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt > 1:316966/1‑71 (MQ=255) accaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagt > 1:351289/3‑71 (MQ=255) accaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagt > 1:3579898/3‑71 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc < 1:1314353/70‑1 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc > 1:2573173/2‑71 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc > 1:2114190/2‑71 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagt < 1:2486406/69‑1 (MQ=255) caagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcg < 1:2758862/71‑2 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca < 1:3896191/71‑1 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca < 1:2711687/71‑1 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc > 1:2867036/1‑70 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc > 1:3703668/1‑70 (MQ=255) aagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga < 1:396480/71‑3 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg > 1:92915/1‑60 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg > 1:3301772/1‑60 (MQ=255) agcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaac > 1:2726355/1‑70 (MQ=255) agcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca > 1:905232/1‑69 (MQ=255) gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag > 1:829777/1‑71 (MQ=255) aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:1383708/71‑1 (MQ=255) aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:2924668/71‑1 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACTAAGGCCAGCCTGGTCAATCAGc > 1:2284306/1‑55 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGc > 1:480434/1‑55 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg < 1:3873835/71‑1 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg < 1:3215487/71‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt < 1:3880562/69‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt < 1:1244384/69‑1 (MQ=255) tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg > 1:637619/1‑69 (MQ=255) tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg > 1:2920819/1‑69 (MQ=255) gcgcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt < 1:913837/54‑1 (MQ=255) gcgcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTa < 1:365271/71‑1 (MQ=255) cgcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc > 1:3863507/1‑43 (MQ=255) gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca < 1:1557665/48‑1 (MQ=255) gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca < 1:2046649/48‑1 (MQ=255) gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca < 1:2684378/48‑1 (MQ=255) gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAcc < 1:2932648/71‑1 (MQ=255) gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAcc < 1:3127684/71‑1 (MQ=255) ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGtt > 1:3950220/1‑62 (MQ=255) ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGtt > 1:1222993/1‑62 (MQ=255) cTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAcc > 1:867461/1‑69 (MQ=255) cTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCa < 1:844486/71‑1 (MQ=255) aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCa > 1:3917331/1‑61 (MQ=255) aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAg < 1:3116239/69‑1 (MQ=255) aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAg < 1:534599/69‑1 (MQ=255) aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt > 1:3010988/1‑71 (MQ=255) aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt > 1:2056128/1‑71 (MQ=255) tCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTc > 1:2475754/1‑70 (MQ=255) cAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:216579/56‑1 (MQ=255) aGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaa < 1:2454866/47‑1 (MQ=255) aGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca > 1:615354/1‑45 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt < 1:1105130/51‑1 (MQ=255) tttCGTGACCAAGGCCCGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGGTGCAGTACCCAGTTCCa > 1:425849/1‑66 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt > 1:3010677/1‑71 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTACACGAtt > 1:2486565/1‑71 (MQ=255) ttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt < 1:468957/70‑1 (MQ=255) gTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAg < 1:1794714/57‑1 (MQ=255) gACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg > 1:910805/1‑42 (MQ=255) gACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGt < 1:3443286/56‑1 (MQ=255) aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACg < 1:872887/61‑1 (MQ=255) aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACg < 1:1040173/61‑1 (MQ=255) aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt < 1:2579081/64‑1 (MQ=255) aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGc < 1:1876877/70‑1 (MQ=255) aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGc < 1:82898/70‑1 (MQ=255) aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCa < 1:3509312/71‑1 (MQ=255) ccAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAg < 1:2932140/71‑1 (MQ=255) cAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTcc > 1:3937777/1‑56 (MQ=255) cAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTcc > 1:362563/1‑56 (MQ=255) cAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAgg > 1:3119178/1‑71 (MQ=255) aGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGtt < 1:2155527/71‑1 (MQ=255) aGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGt > 1:2484394/1‑70 (MQ=255) cAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTaaa < 1:3499212/70‑1 (MQ=255) gTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAg > 1:1623697/1‑39 (MQ=255) tCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCa < 1:670322/55‑1 (MQ=255) tCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAgg > 1:3269946/1‑57 (MQ=255) tCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAAc < 1:3302760/71‑1 (MQ=255) aaTCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTaa < 1:3855605/59‑1 (MQ=255) aaTCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTGAAGGCGAAAACCa > 1:2266626/1‑71 (MQ=255) aaTCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCa > 1:82497/1‑71 (MQ=255) aaTCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCa < 1:1426591/71‑1 (MQ=255) tCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCggg < 1:459417/49‑1 (MQ=255) tCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTaaa < 1:2942743/58‑1 (MQ=255) tCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATc > 1:2998070/1‑71 (MQ=255) tCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATc > 1:95307/1‑71 (MQ=255) | CGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATC > minE/310752‑310890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |