Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,941,702 T→G 29.2% intergenic (+141/+55) ygfZ → / ← yqfA predicted folate‑dependent regulatory protein/predicted oxidoreductase, inner membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,941,7020TG29.2% 72.4 / 64.8 77intergenic (+141/+55)ygfZ/yqfApredicted folate‑dependent regulatory protein/predicted oxidoreductase, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (24/30);  new base G (10/12);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

ATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAA  >  minE/1941639‑1941770
                                                               |                                                                     
aTCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATg                                                                 <  1:346469/70‑1 (MQ=255)
aTCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGc                                                                >  1:2374481/1‑71 (MQ=255)
 tCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCg                                                                    <  1:2804215/66‑1 (MQ=255)
   acaacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTg                                                             <  1:3901166/71‑1 (MQ=255)
    caacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTg                                                             <  1:2732458/70‑1 (MQ=255)
      acaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATg                                                          >  1:3508359/1‑71 (MQ=255)
       caGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGc                                                         <  1:3592745/71‑1 (MQ=255)
         gggACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGa                                                       <  1:3009107/71‑1 (MQ=255)
          ggACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGa                                                            <  1:1252176/65‑1 (MQ=255)
          ggACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGa                                                            <  1:3084413/65‑1 (MQ=255)
               ggatcggtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTgg                                                >  1:3895929‑M2/34‑71 (MQ=255)
                ggCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc                                               <  1:214341/71‑1 (MQ=255)
                gatcggtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGgc                                               >  1:1389578‑M2/33‑71 (MQ=255)
                gatcggtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGgc                                               <  1:1573900‑M2/39‑1 (MQ=255)
                gatcggtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGgc                                               <  1:3416521‑M2/39‑1 (MQ=255)
                  cccGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcgc                                             <  1:1035159/71‑1 (MQ=255)
                    cGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc                                               >  1:721399/1‑67 (MQ=255)
                     gtaggccggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTc                                          <  1:2360204‑M2/44‑1 (MQ=255)
                     gTAGATCGGATAAGGCTTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                          <  1:680415/71‑1 (MQ=255)
                     gTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTg                                                 >  1:1105797/1‑64 (MQ=255)
                     gTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcgc                                             >  1:1006972/1‑68 (MQ=255)
                     gTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                          >  1:3656583/1‑71 (MQ=255)
                      tAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCt                                         >  1:3580549/1‑71 (MQ=255)
                      tAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCt                                         >  1:1806659/1‑71 (MQ=255)
                       aGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCt                                         >  1:343487/1‑70 (MQ=255)
                       aGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCt                                         <  1:1056558/70‑1 (MQ=255)
                       aGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCt                                         >  1:870230/1‑70 (MQ=255)
                        ggccggataaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCtt                                        <  1:133151‑M2/46‑1 (MQ=255)
                        gATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCtt                                        <  1:3064611/70‑1 (MQ=255)
                         gacggataaggcgtttacgccgcATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTAt                                      <  1:1662265‑M2/48‑1 (MQ=255)
                         gacggataaggcgtttacgccgcATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTAt                                      <  1:2368052‑M2/48‑1 (MQ=255)
                          ccggaaaaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGcc                                                               >  1:648346‑M2/23‑46 (MQ=255)
                           cggataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCa                                    >  1:987682‑M2/22‑71 (MQ=255)
                           cggataaggcgttcacgccgcATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATc                                     <  1:2904295‑M2/49‑1 (MQ=255)
                           cGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc                                               <  1:2766836/60‑1 (MQ=255)
                            ggataaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAg                                   <  1:1554587‑M2/51‑1 (MQ=255)
                            ggATAAGGTGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAg                                   >  1:301015/1‑71 (MQ=255)
                            ggATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAg                                   <  1:101681/71‑1 (MQ=255)
                            ggATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAg                                   >  1:3441355/1‑71 (MQ=255)
                             gataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTAtcatg                                  <  1:1401336‑M2/52‑3 (MQ=255)
                             gataaggcgtttatgccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTAtcatg                                  >  1:3861018‑M2/20‑69 (MQ=255)
                             gATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTTGCGCGTCTTATCAgg                                  >  1:3149341/1‑71 (MQ=255)
                              ataaggcgttcacgccgcATCCGGCAGTTGTGCACCAATgct                                                               <  1:3276618‑M2/24‑2 (MQ=255)
                              aTAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                 >  1:2086588/1‑71 (MQ=255)
                               taaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCt                                                  >  1:283239‑M2/18‑53 (MQ=255)
                               taaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGAcc                                <  1:3457494‑M2/54‑1 (MQ=255)
                                aaggcgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGAcc                                >  1:230984‑M2/17‑70 (MQ=255)
                                  ggCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                              >  1:3348871/1‑70 (MQ=255)
                                   gcgttcacgccgcATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTAc                             <  1:877651‑M2/57‑1 (MQ=255)
                                    cgttcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACaa                           <  1:1261151‑M2/59‑1 (MQ=255)
                                    cGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaa                           <  1:2028191/71‑1 (MQ=255)
                                    cGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaa                           <  1:803310/71‑1 (MQ=255)
                                     gTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaaa                          <  1:225786/71‑1 (MQ=255)
                                     gTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                            >  1:1005245/1‑69 (MQ=255)
                                      ttCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAg                         <  1:1800337/71‑1 (MQ=255)
                                      ttCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAg                         >  1:3858277/1‑71 (MQ=255)
                                       tcacgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTacaaatt                        >  1:3835603‑M2/10‑69 (MQ=255)
                                       tCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGAt                                                           <  1:1322856/37‑1 (MQ=255)
                                        cACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAgg                        >  1:335590/1‑70 (MQ=255)
                                        cACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAgg                        >  1:370796/1‑70 (MQ=255)
                                         aCGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCa                      >  1:2875286/1‑71 (MQ=255)
                                            ccgcATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                             >  1:876036‑M2/5‑61 (MQ=255)
                                            ccGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATAc                   >  1:2790125/1‑71 (MQ=255)
                                             cgcATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTacg                            >  1:3914912‑M2/4‑60 (MQ=255)
                                             cGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATAcc                  >  1:3800541/1‑71 (MQ=255)
                                               aaTCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCa                >  1:2383334/1‑71 (MQ=255)
                                                 tCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCAtt              <  1:1176943/71‑1 (MQ=255)
                                                   cGGCAGTTGTGCTCCGATGCCTGATGTGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTAc            <  1:413252/71‑1 (MQ=255)
                                                   cGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTAc            <  1:3625777/71‑1 (MQ=255)
                                                   cGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTAc            <  1:2323387/71‑1 (MQ=255)
                                                      cAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCCTACCCATTa             >  1:584861/1‑67 (MQ=255)
                                                         ttGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaa                           <  1:1915082/50‑1 (MQ=255)
                                                           gtgCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATAc                   <  1:61827/56‑1 (MQ=255)
                                                           gtgCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATAc                   <  1:1648832/56‑1 (MQ=255)
                                                            tgCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCa   <  1:579702/71‑1 (MQ=255)
                                                              cTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCaa  <  1:2030895/70‑1 (MQ=255)
                                                              cTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCaa  <  1:2755114/70‑1 (MQ=255)
                                                               |                                                                     
ATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAA  >  minE/1941639‑1941770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: