Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,960,125 G→A 12.0% intergenic (‑55/+160) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,960,1250GA12.0% 137.9 / 8.3 50intergenic (‑55/+160)fbaA/pgkfructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base A (3/3);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

AAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCT  >  minE/1960057‑1960195
                                                                    |                                                                      
aaaaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGtgct                                                                      >  1:1898844/1‑71 (MQ=255)
aaaaTCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGtgct                                                                      <  1:3412705/71‑1 (MQ=255)
         gACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtctgcg                                                             <  1:1387194/71‑1 (MQ=255)
            aTGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcttctg                                                               >  1:2993118/1‑66 (MQ=255)
            aTGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgt                                                                  <  1:3696645/63‑1 (MQ=255)
              gTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtct                                                                >  1:521245/1‑63 (MQ=255)
              gTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtct                                                                >  1:3310808/1‑63 (MQ=255)
              gTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtct                                                                >  1:3341252/1‑63 (MQ=255)
              gTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc                                                        >  1:1077152/1‑71 (MQ=255)
               tctcTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt                                                       <  1:2318008/71‑1 (MQ=255)
               tctcTTGTCCTGTAGCGTCGGGCCTTATAATCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt                                                       <  1:276851/71‑1 (MQ=255)
                ctctTGTCCTGTATCGTCGGGCCTTATACTCGTTATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt                                                       >  1:3728762/1‑70 (MQ=255)
                ctctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGtgc                                                                       >  1:3641451/1‑54 (MQ=255)
                ctctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGtgc                                                                       >  1:799435/1‑54 (MQ=255)
                ctctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGtgc                                                                       >  1:3307642/1‑54 (MQ=255)
                ctctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc                                                        >  1:1026831/1‑69 (MQ=255)
                    tGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcc                                                   >  1:3844460/1‑70 (MQ=255)
                     gTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                                                  <  1:1725567/70‑1 (MQ=255)
                     gTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAc                                                    <  1:629279/68‑1 (MQ=255)
                       ccTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcc                                                   >  1:1938988/1‑67 (MQ=255)
                          gTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa                                            >  1:1306025/1‑71 (MQ=255)
                          gTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa                                            >  1:2949818/1‑71 (MQ=255)
                          gTATCGTCGGGCCTTATACTCGGCATACTTCAAGTTGCATGTGCTGCGGCTGCGTTCGc                                                        <  1:1544539/59‑1 (MQ=18)
                           tATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTc                                             <  1:2817802/69‑1 (MQ=255)
                             tcgtcgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactt                                         <  1:1626264/71‑1 (MQ=255)
                                  gggCCTTATACTCGTCATACTTCAAGTTGCATGtgctgc                                                                    >  1:706506/1‑39 (MQ=255)
                                   ggCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttacat                                     >  1:219610/1‑67 (MQ=255)
                                    gCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATg                                  >  1:3000152/1‑71 (MQ=40)
                                    catttATACTCGTCATACTTCAAGTTTCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTAt                                   <  1:1508903/67‑1 (MQ=11)
                                    catttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTAt                                   <  1:3171209/67‑1 (MQ=11)
                                    catttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATg                                  >  1:2087616/4‑71 (MQ=11)
                                     ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt                                 <  1:659104/71‑1 (MQ=39)
                                      tttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCa                                                     <  1:1398768/49‑1 (MQ=11)
                                      tttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGt                                 >  1:3319782/2‑70 (MQ=11)
                                      cTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTa                                <  1:2442243/71‑1 (MQ=38)
                                       ttATACTCGTCATACTTCAAGTTGCATGTGctgcgtc                                                                 >  1:3049005/1‑37 (MQ=40)
                                       ttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCa                                            >  1:2032955/1‑58 (MQ=11)
                                        tataCTCGTCATACTTCAAGTTGCATGTGctgcgtctgcg                                                             <  1:932383/40‑1 (MQ=40)
                                        tataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAg                              >  1:1982469/1‑71 (MQ=34)
                                        tataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAg                              <  1:528091/71‑1 (MQ=34)
                                         ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta                                        >  1:2991028/1‑60 (MQ=35)
                                         ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta                                        >  1:724298/1‑60 (MQ=35)
                                         ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTaa                               >  1:325241/1‑69 (MQ=35)
                                          taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                                                  <  1:3448954/49‑1 (MQ=35)
                                          taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc                             <  1:993451/70‑1 (MQ=33)
                                          taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCt                            <  1:182209/71‑1 (MQ=32)
                                           aCTCGTCATACTTCAAGTTGCATGTGctgcgtctg                                                               <  1:403607/35‑1 (MQ=33)
                                           aCTCGTCATACTTCAAGTTGCATGTGctgcgtctg                                                               <  1:3248387/35‑1 (MQ=33)
                                           aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa                                                >  1:3700598/1‑50 (MQ=32)
                                           aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa                                                >  1:1284439/1‑50 (MQ=32)
                                           aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa                                                >  1:1160582/1‑50 (MQ=32)
                                           aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTaa                               <  1:983832/67‑1 (MQ=31)
                                                           gTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCActctc           >  1:2531184/1‑71 (MQ=32)
                                                           gTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCActctc           <  1:812104/71‑1 (MQ=32)
                                                              gCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCtt         <  1:3810529/70‑1 (MQ=35)
                                                                aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTc      <  1:3420675/71‑1 (MQ=34)
                                                                    gctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCt  >  1:3168874/1‑71 (MQ=34)
                                                                    |                                                                      
AAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCT  >  minE/1960057‑1960195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: