| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,960,141 | C→T | 13.0% | intergenic (‑71/+144) | fbaA ← / ← pgk | fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,960,141 | 0 | C | T | 13.0% | 125.2 / 8.5 | 46 | intergenic (‑71/+144) | fbaA/pgk | fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase |
| Reads supporting (aligned to +/- strand): ref base C (22/18); new base T (3/3); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
GTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTC > minE/1960071‑1960206 | gTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc > 1:1077152/1‑71 (MQ=255) tctcTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt < 1:2318008/71‑1 (MQ=255) tctcTTGTCCTGTAGCGTCGGGCCTTATAATCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt < 1:276851/71‑1 (MQ=255) ctctTGTCCTGTATCGTCGGGCCTTATACTCGTTATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt > 1:3728762/1‑70 (MQ=255) ctctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc > 1:1026831/1‑69 (MQ=255) tGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcc > 1:3844460/1‑70 (MQ=255) gTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc < 1:1725567/70‑1 (MQ=255) gTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAc < 1:629279/68‑1 (MQ=255) ccTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcc > 1:1938988/1‑67 (MQ=255) gTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa > 1:2949818/1‑71 (MQ=255) gTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa > 1:1306025/1‑71 (MQ=255) gTATCGTCGGGCCTTATACTCGGCATACTTCAAGTTGCATGTGCTGCGGCTGCGTTCGc < 1:1544539/59‑1 (MQ=18) tATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTc < 1:2817802/69‑1 (MQ=255) tcgtcgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactt < 1:1626264/71‑1 (MQ=255) ggCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttacat > 1:219610/1‑67 (MQ=255) gCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATg > 1:3000152/1‑71 (MQ=40) catttATACTCGTCATACTTCAAGTTTCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTAt < 1:1508903/67‑1 (MQ=11) catttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTAt < 1:3171209/67‑1 (MQ=11) catttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATg > 1:2087616/4‑71 (MQ=11) ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt < 1:659104/71‑1 (MQ=39) tttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCa < 1:1398768/49‑1 (MQ=11) tttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGt > 1:3319782/2‑70 (MQ=11) cTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTa < 1:2442243/71‑1 (MQ=38) ttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCa > 1:2032955/1‑58 (MQ=11) tataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAg < 1:528091/71‑1 (MQ=34) tataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAg > 1:1982469/1‑71 (MQ=34) ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta > 1:724298/1‑60 (MQ=35) ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta > 1:2991028/1‑60 (MQ=35) ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTaa > 1:325241/1‑69 (MQ=35) taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc < 1:3448954/49‑1 (MQ=35) taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc < 1:993451/70‑1 (MQ=33) taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCt < 1:182209/71‑1 (MQ=32) aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa > 1:1160582/1‑50 (MQ=32) aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa > 1:1284439/1‑50 (MQ=32) aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa > 1:3700598/1‑50 (MQ=32) aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTaa < 1:983832/67‑1 (MQ=31) gTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCActctc < 1:812104/71‑1 (MQ=32) gTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCActctc > 1:2531184/1‑71 (MQ=32) gCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCtt < 1:3810529/70‑1 (MQ=35) aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTc < 1:3420675/71‑1 (MQ=34) gctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCt > 1:3168874/1‑71 (MQ=34) ctgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCtt > 1:1036523/1‑71 (MQ=34) gtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCt > 1:1946594/1‑66 (MQ=35) ctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGc > 1:2874660/1‑70 (MQ=35) tgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCaa > 1:2932855/1‑71 (MQ=34) cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCa < 1:656424/68‑1 (MQ=35) cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAAc < 1:3332329/70‑1 (MQ=35) cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACt > 1:3311305/1‑71 (MQ=34) ttCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTc > 1:1636664/1‑70 (MQ=35) | GTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTC > minE/1960071‑1960206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |