| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,963,700 | C→T | 8.0% | A108T (GCC→ACC) | yggD ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,963,700 | 0 | C | T | 8.0% | 221.5 / 7.2 | 75 | A108T (GCC→ACC) | yggD | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (19/50); new base T (3/3); total (22/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
CGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCGTA > minE/1963631‑1963769 | cgTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGc < 1:3852976/70‑1 (MQ=255) gTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCaa < 1:34411/71‑1 (MQ=255) tCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCaaa < 1:3375317/71‑1 (MQ=255) aTAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATc < 1:258728/71‑1 (MQ=255) tAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATcc < 1:673926/71‑1 (MQ=255) tAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATcc < 1:1474884/71‑1 (MQ=255) agagCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATcc > 1:1253237/1‑70 (MQ=255) agCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCggg > 1:3128717/1‑71 (MQ=255) cATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATc > 1:959717/1‑65 (MQ=255) tCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGAGtt > 1:666216/1‑71 (MQ=255) tCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGAGt < 1:2317433/70‑1 (MQ=255) ttGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATc > 1:335311/1‑71 (MQ=255) ggTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgtt > 1:1604698/1‑71 (MQ=255) ggTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgt > 1:767515/1‑70 (MQ=255) ttATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATc < 1:2746269/51‑1 (MQ=255) ttATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcg < 1:3817167/71‑1 (MQ=255) tGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgttc < 1:942210/71‑1 (MQ=255) cTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCa < 1:3570027/70‑1 (MQ=255) cTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCa < 1:419218/70‑1 (MQ=255) cTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAg < 1:3186307/71‑1 (MQ=255) tATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCa < 1:2827323/69‑1 (MQ=255) tATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCa < 1:2822803/69‑1 (MQ=255) aTTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAgg > 1:762154/1‑70 (MQ=255) aTTGACCTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGATCGTTCAgg > 1:766809/1‑70 (MQ=255) ttGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGt < 1:2807129/50‑1 (MQ=255) tGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGttt < 1:513643/51‑1 (MQ=255) gACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAg > 1:3539354/1‑66 (MQ=255) aCGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATcc < 1:2498111/54‑1 (MQ=255) aCGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAAt < 1:3751539/70‑1 (MQ=255) gTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgttc < 1:3665195/61‑1 (MQ=255) gTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGcc < 1:540453/71‑1 (MQ=255) tGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATc > 1:1244797/1‑49 (MQ=255) gCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCa > 1:3721264/1‑46 (MQ=255) cTGATAAAATCCCAGGTCATGTCATCGGTAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGaa < 1:378287/71‑1 (MQ=255) cTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGaa < 1:2638948/71‑1 (MQ=255) gATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTa < 1:609926/60‑1 (MQ=255) aTAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGtt > 1:3215399/1‑39 (MQ=255) aTAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttc < 1:2356740/50‑1 (MQ=255) aTAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttc < 1:2274384/50‑1 (MQ=255) aTAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAAc < 1:1656927/69‑1 (MQ=255) aTAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAAc < 1:3587071/69‑1 (MQ=255) tAAAATCCCAGGTCATGTCATCGGTAAATCCGGGCGt < 1:1858338/37‑1 (MQ=255) aaaTCCCAGGTCATGTCATCGGTAAATCCGGGCGttt > 1:1970418/1‑37 (MQ=255) aaaTCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAg > 1:1706389/1‑53 (MQ=255) aaaTCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTggg < 1:194239/70‑1 (MQ=255) aaaTCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTggg < 1:3583405/70‑1 (MQ=255) aaTCCCAGGTCATGTCATCGGCAAATCCGGGCGttt > 1:913086/1‑36 (MQ=255) aTCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGaaa < 1:3684159/71‑1 (MQ=255) aTCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGaa > 1:2675730/1‑70 (MQ=255) tCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGaaaa < 1:2105668/71‑1 (MQ=255) tCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAAAGCCAGAACTGGGaaaa < 1:1255528/71‑1 (MQ=255) ccAGGTCATGTCATCGGTAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAAt < 1:3532042/71‑1 (MQ=255) ccAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAAt < 1:2350700/71‑1 (MQ=255) cAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATg > 1:2885701/1‑71 (MQ=255) ggTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCg < 1:3842758/71‑1 (MQ=255) ggTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCg < 1:1447015/71‑1 (MQ=255) ggTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCg > 1:532817/1‑71 (MQ=255) gtcatgtcatCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGt < 1:349033/71‑1 (MQ=255) tcatgtcatCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGt < 1:2706963/70‑1 (MQ=255) atgtcatCGGCAAATCCGGGCGTTTCATCCTgttcgt < 1:485416/37‑1 (MQ=255) atgtcatCGGCAAATCCGGGCGTTTCATCCTgttcgt < 1:875547/37‑1 (MQ=255) atgtcatCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGa < 1:3035662/70‑1 (MQ=255) tgtcatCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATg < 1:511820/64‑1 (MQ=255) tgtcatCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGata < 1:3054990/71‑1 (MQ=255) gtcatCGGTAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAAt > 1:2564093/1‑44 (MQ=255) gtcatCGGTAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAAt > 1:1839993/1‑44 (MQ=255) tcatCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGatat < 1:2658681/70‑1 (MQ=255) tcatCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATc < 1:2183907/71‑1 (MQ=255) tcatCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATc < 1:2103992/71‑1 (MQ=255) atCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCg < 1:1182503/70‑1 (MQ=255) tCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGt < 1:608384/37‑1 (MQ=255) tCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGaaa < 1:621017/55‑1 (MQ=255) tCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGaaa < 1:3477840/55‑1 (MQ=255) ggCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCg > 1:365244/1‑70 (MQ=255) ggCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCGt < 1:627211/71‑1 (MQ=255) gCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCGTa < 1:817889/71‑1 (MQ=255) | CGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCGTA > minE/1963631‑1963769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |