Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,990,234	A→G	50.0%	L76L (TTA→TTG)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,990,234	0	A	G	50.0%	‑5.1 / 63.9	46	L76L (TTA→TTG)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base A (7/16);  new base G (12/11);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAGTGGCGACCTTA  >  minE/1990168‑1990303                                                                   |                                                                      cTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGa                                                                   <  1:1760311/71‑1 (MQ=255)   ttATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATg                                                                     <  1:3740734/67‑1 (MQ=255)       ggCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGAc                                                                  <  1:3330777/66‑1 (MQ=255)        gcgcgcTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCt                                                            <  1:3900948/71‑1 (MQ=255)         cgcgcTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCtt                                                           <  1:426282/71‑1 (MQ=255)         cgcgcTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCt                                                            <  1:1276412/70‑1 (MQ=255)          gcgcTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTg                                                          <  1:2786986/71‑1 (MQ=255)          gcgcTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTg                                                          >  1:641292/1‑71 (MQ=255)                cccGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTa                                                      <  1:1745030/69‑1 (MQ=255)                cccGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTa                                                      <  1:1990219/69‑1 (MQ=255)                cccGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTa                                                      <  1:3600130/69‑1 (MQ=255)                 ccGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTAtt                                                    <  1:69435/70‑1 (MQ=255)                 ccGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTAtt                                                    <  1:456119/70‑1 (MQ=255)                 ccGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTAtt                                                    <  1:99599/70‑1 (MQ=255)                  cGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTATTAc                                                  <  1:1319700/71‑1 (MQ=255)                  cGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTATTAc                                                  <  1:3186155/71‑1 (MQ=255)                  cGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTATTAc                                                  <  1:3181962/71‑1 (MQ=255)                  cGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTATTAc                                                  >  1:2362965/1‑71 (MQ=255)                    aCGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTATTACGc                                                >  1:2627399/1‑71 (MQ=255)                     cGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTa                                                   >  1:3006307/1‑67 (MQ=255)                       gTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCgg                                                               >  1:3805527/1‑53 (MQ=255)                        tGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCcgc                                            <  1:811218/71‑1 (MQ=255)                        tGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGAcc                                                                 <  1:3219363/50‑1 (MQ=255)                          aCCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCcgcgc                                          >  1:2726351/1‑71 (MQ=255)                          aCCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTATTACGCCcgcgc                                          <  1:409506/71‑1 (MQ=255)                           ccGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCcgcgc                                          <  1:2098411/70‑1 (MQ=255)                           ccGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCg                                                                >  1:557625/1‑48 (MQ=255)                            cGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTAt                                                     <  1:2065777/58‑1 (MQ=255)                            cGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTATTACGCCcgcgcgc                                        >  1:3211274/1‑71 (MQ=255)                              aTCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGGTAtt                                                    >  1:3219933/1‑57 (MQ=255)                              aTCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCt                                                            <  1:887654/49‑1 (MQ=255)                                  cGCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTAt                                                     >  1:732387/1‑52 (MQ=255)                                   gCCAATGCGCCGCTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCt                                 >  1:287271/1‑71 (MQ=255)                                          cgccgcTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATaaa                          <  1:1933146/71‑1 (MQ=255)                                           gccgcTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAg                         <  1:1165244/71‑1 (MQ=255)                                               cTCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCgg                      >  1:3542709/1‑70 (MQ=255)                                                tCGACGAAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGca                    >  1:464942/1‑71 (MQ=255)                                                  gacgaAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATc                                  <  1:2484625/55‑1 (MQ=255)                                                     gaAGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCacaac                >  1:3703858/1‑70 (MQ=255)                                                     gaAGTTCTCCACTTATGGACCGGGCTTGGCTATTACGCCcgcgcgcg                                       <  1:609633/47‑1 (MQ=255)                                                       aGTTCTCCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAg             >  1:2090507/1‑71 (MQ=255)                                                       aGTTCTCCACTTATGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAg             >  1:725599/1‑71 (MQ=255)                                                       aGTTCTCCACTTATGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAg             <  1:908587/71‑1 (MQ=255)                                                          tctcCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCTCGCAATCTGCATAAAGCGGCACAACAAGTgg          >  1:1294529/1‑71 (MQ=255)                                                            tcCACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAGTGGc         >  1:3849105/1‑70 (MQ=255)                                                              cACTTGTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAGTGGCGAc      >  1:1157257/1‑71 (MQ=255)                                                                   aTGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAGTGGCGACCTTa  >  1:1891446/1‑70 (MQ=255)                                                                   |                                                                      CTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCCACTTATGGACCGGGCTTGGCTATTACGCCCGCGCGCGCAATCTGCATAAAGCGGCACAACAAGTGGCGACCTTA  >  minE/1990168‑1990303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑