| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,008,716 | T→C | 5.8% | K102K (AAA→AAG) | hybO ← | hydrogenase 2, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,008,716 | 0 | T | C | 5.8% | 194.2 / 2.4 | 69 | K102K (AAA→AAG) | hybO | hydrogenase 2, small subunit |
| Reads supporting (aligned to +/- strand): ref base T (23/42); new base C (2/2); total (25/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
ATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCC > minE/2008647‑2008785 | aTCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgt > 1:231648/1‑70 (MQ=255)aTCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgt < 1:1350584/70‑1 (MQ=255) tCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtt > 1:601024/1‑70 (MQ=255) tCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtt < 1:406831/70‑1 (MQ=255) tttAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttgt > 1:1835034/1‑70 (MQ=255) ttAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgttt > 1:803067/1‑67 (MQ=255) tAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTtc < 1:1473791/70‑1 (MQ=255) aTGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTtct < 1:1162753/69‑1 (MQ=255) ggATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTc < 1:35525/70‑1 (MQ=255) ggATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCg < 1:2198104/71‑1 (MQ=255) gATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGa < 1:903197/71‑1 (MQ=255) aTGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtt < 1:2115316/58‑1 (MQ=255) tGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGAcc < 1:3083264/71‑1 (MQ=255) gAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCtg < 1:1187964/71‑1 (MQ=255) aaCCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCtg < 1:2925256/70‑1 (MQ=255) aaCCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGCTTGTTCTCTTCGACCtga > 1:3815521/1‑71 (MQ=255) aCCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCtgat < 1:1659327/71‑1 (MQ=255) aCCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCtgat < 1:944460/71‑1 (MQ=255) ccATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgt > 1:2591741/1‑51 (MQ=255) ccATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgt > 1:2234938/1‑51 (MQ=255) ccATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCtgatg < 1:112991/71‑1 (MQ=255) cATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCtga < 1:1509735/68‑1 (MQ=255) tCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtt > 1:2047519/1‑49 (MQ=255) ccacacTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCg > 1:1136389/4‑70 (MQ=255) caccacTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGa < 1:1421039/71‑1 (MQ=255) accacTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttg < 1:2191009/48‑1 (MQ=255) acTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttg > 1:79502/1‑45 (MQ=255) acTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggc > 1:2438029/1‑71 (MQ=255) cTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTGTGTTTGTTCTCTTCGACCtg < 1:1388431/58‑1 (MQ=255) cTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTtctc < 1:210122/49‑1 (MQ=255) acacATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTtct < 1:971273/45‑1 (MQ=255) acacATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCtt < 1:3784477/48‑1 (MQ=255) acacATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCg < 1:3771554/50‑1 (MQ=255) acacATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCt < 1:2096855/54‑1 (MQ=255) acacATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGgcggcg > 1:2700897/1‑71 (MQ=255) cacaTACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggcgg < 1:3169603/68‑1 (MQ=255) cacaTACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGcggcgg < 1:2256705/71‑1 (MQ=255) acaTACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGa < 1:1228325/71‑1 (MQ=255) cacaCTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGaa < 1:1366125/68‑1 (MQ=255) caTACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggcggc > 1:166145/1‑67 (MQ=255) caTACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGa < 1:1287523/70‑1 (MQ=255) caTACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGa > 1:3629257/1‑70 (MQ=255) aTACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttgt < 1:1903489/38‑1 (MQ=255) aTACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttgt < 1:790964/38‑1 (MQ=255) aTACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttgt < 1:1898941/38‑1 (MQ=255) aTACTGCCCTTTGTACTTCTCGAGAGCGTTATGCTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGa > 1:2035965/1‑69 (MQ=255) aCTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTtc > 1:242463/1‑38 (MQ=255) cTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCg < 1:1282077/43‑1 (MQ=255) tGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCAc > 1:303198/1‑71 (MQ=255) gCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACt > 1:303455/1‑71 (MQ=255) cccTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACtt < 1:3662327/71‑1 (MQ=255) cccTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACtt < 1:3190074/71‑1 (MQ=255) cccTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACt < 1:2462791/70‑1 (MQ=255) cTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGc > 1:1492222/1‑65 (MQ=255) cTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCg > 1:1448836/1‑71 (MQ=255) tttGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGAcc < 1:2096882/40‑1 (MQ=255) tttGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCtgat < 1:2672840/44‑1 (MQ=255) tttGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGaa < 1:1736319/51‑1 (MQ=255) tttGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGt > 1:2762159/1‑71 (MQ=255) tttGTACTTCTCGAGAGCGTTATGCTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCa < 1:3936076/65‑1 (MQ=255) tGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGAc > 1:1985273/1‑37 (MQ=255) tGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAgg > 1:3933897/1‑51 (MQ=255) tGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCAc > 1:2006111/1‑64 (MQ=255) tGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGt < 1:2450982/69‑1 (MQ=255) tGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGa > 1:3481965/1‑71 (MQ=255) gTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGa < 1:3522687/70‑1 (MQ=255) tACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGa > 1:2466745/1‑46 (MQ=255) tACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGa < 1:3288367/56‑1 (MQ=255) cTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATAc < 1:501348/70‑1 (MQ=255) ttCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGaa < 1:933286/54‑1 (MQ=255) ttCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTc > 1:1872417/1‑71 (MQ=255) tctcGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTc > 1:2071974/1‑70 (MQ=255) ctcGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAg > 1:2299579/1‑54 (MQ=255) ctcGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTc > 1:2244729/1‑69 (MQ=255) ctcGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTc < 1:3356018/69‑1 (MQ=255) ctcGAGAGCGTTATGCTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTc < 1:3250036/69‑1 (MQ=255) gagaGCGTTATGTTTGTTCTCTTCGACCTGATGACCGa < 1:1889662/38‑1 (MQ=255) gagaGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCa > 1:1791988/1‑68 (MQ=255) agCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAgagaga < 1:2740351/71‑1 (MQ=255) cGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATa < 1:2246740/71‑1 (MQ=255) gTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCagaga < 1:525385/66‑1 (MQ=255) gTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCagaga < 1:608650/66‑1 (MQ=255) aTGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtct > 1:2170018/1‑71 (MQ=255) aTGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtct > 1:1385984/1‑71 (MQ=255) tgtttgttCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtctc > 1:3009472/1‑71 (MQ=255) tttgttCTCTTCGACCTGATGACCGAAGGCGGCGGa < 1:1078218/36‑1 (MQ=255) tttgttCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTc < 1:3400284/45‑1 (MQ=255) tttgttCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTc < 1:2509737/45‑1 (MQ=255) tttgttCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtctc < 1:1658724/69‑1 (MQ=255) tttgttCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTcc < 1:2060024/70‑1 (MQ=255) | ATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCC > minE/2008647‑2008785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |