| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,013,468 | A→T | 14.3% | intergenic (+162/+110) | yghA → / ← exbD | predicted glutathionylspermidine synthase, with NAD(P)‑binding Rossmann‑fold domain/membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,013,468 | 0 | A | T | 14.3% | 169.4 / 18.7 | 73 | intergenic (+162/+110) | yghA/exbD | predicted glutathionylspermidine synthase, with NAD(P)‑binding Rossmann‑fold domain/membrane spanning protein in TonB‑ExbB‑ExbD complex |
| Reads supporting (aligned to +/- strand): ref base A (45/15); new base T (6/3); total (52/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
TCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGAT > minE/2013405‑2013536 | tCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGa > 1:716606/1‑71 (MQ=255) aCATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGc < 1:482222/71‑1 (MQ=255) cATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGcg < 1:1552784/71‑1 (MQ=255) aTGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGa > 1:343130/1‑66 (MQ=255) gTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGcgtcg > 1:983786/1‑71 (MQ=255) ttGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGcgtcgt < 1:3222267/71‑1 (MQ=255) tGCAATAGATTTTGTAGGCCTGATAAGCGTAGAGCATCAGGCATTGAGCACCTAATGCCGGATGcgtcgt > 1:172582/1‑70 (MQ=255) gCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTg < 1:90943/70‑1 (MQ=255) cAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGa < 1:3608013/70‑1 (MQ=255) aTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAAc < 1:1029503/70‑1 (MQ=255) aTTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCtt < 1:1374020/71‑1 (MQ=255) ttttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTa < 1:3422601/71‑1 (MQ=255) tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTa < 1:2858905/70‑1 (MQ=255) tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTa > 1:2200219/1‑70 (MQ=255) tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTa < 1:3774097/70‑1 (MQ=255) tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTAt > 1:3264642/1‑71 (MQ=255) ctGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACC‑ATTGCCGGATGCGGCGTGAACGCCTTATcc > 1:3593501/2‑71 (MQ=14) tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACg > 1:1459170/1‑63 (MQ=255) tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATc < 1:408657/70‑1 (MQ=255) gTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCg > 1:83353/1‑71 (MQ=255) tAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACC‑ATTGCCGGATGCGGCGTGAACGCCTTATc < 1:1368354/67‑1 (MQ=14) tAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACC‑ATTGCCGGATGCGGCGTGAACGCCTTATc < 1:2163813/67‑1 (MQ=14) tAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACC‑ACTGCCGGATGCGGCGTGGACGCCTTATcc < 1:2892251/68‑1 (MQ=14) tAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACC‑ACTGCCGGATGCGGCGTGGACGCCTTATc < 1:3696935/67‑1 (MQ=14) tAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACC‑ACTGCCGGATGCGGCGTGGACGCCTTATc < 1:1182418/67‑1 (MQ=14) aGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACC‑ATTGCCGGATGCGGCGTGAACGCCTTATc > 1:309970/1‑66 (MQ=14) aGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGcgtc > 1:353167/1‑53 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCATTGAGAACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGc > 1:517085/1‑71 (MQ=255) ggCCTGATAAGCGTAGCGCATCAGGCATTTGTCACC‑ATTGCCGGATGCGGCGTGAACGCCTTATCCGGcc > 1:2894970/1‑70 (MQ=14) ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGc > 1:3596814/1‑70 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGcc > 1:1356117/1‑70 (MQ=255) ccTGATAAGCGTAGCGCATCAGGCATTTGTCACC‑ATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa > 1:1120287/1‑70 (MQ=14) ccTGATAAGCGTAGCGCATCAGGCATTTGTCACC‑ATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc < 1:3237446/71‑1 (MQ=14) cTGATAAGCGTAGCGCATCAGGCATTTGTCACC‑ATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc > 1:2008421/1‑70 (MQ=11) cTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTa > 1:2139927/1‑70 (MQ=255) tGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTAc > 1:103821/1‑70 (MQ=255) tGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTAc > 1:917597/1‑70 (MQ=255) gATAAGCGTAGCGCATCAGGCATTTGCCACC‑ACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTAcgag > 1:1688264/1‑68 (MQ=2) gATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACa > 1:1284305/1‑70 (MQ=255) gATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACa > 1:2907219/1‑70 (MQ=255) aTAAGCGTAGCGCATCAGGCATTTGTCACC‑ATTGCCGGATGCGGCGTGAACGCCtt > 1:1902853/1‑56 (MQ=2) aTAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACaaa > 1:2412091/1‑71 (MQ=255) tAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAc > 1:1491971/1‑71 (MQ=255) aGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACaaa > 1:2414028/1‑68 (MQ=255) aGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACg > 1:429728/1‑70 (MQ=255) gCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCa > 1:2867528/1‑71 (MQ=255) gCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCa > 1:616682/1‑71 (MQ=255) gCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCa > 1:263891/1‑71 (MQ=255) gCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCa > 1:2823105/1‑71 (MQ=255) cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCg < 1:2575131/57‑1 (MQ=255) cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCa > 1:884712/1‑70 (MQ=255) gTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCAc > 1:3109714/1‑70 (MQ=255) tAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACg > 1:1324369/1‑70 (MQ=255) tAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACg > 1:1517235/1‑70 (MQ=255) tAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACg > 1:911944/1‑70 (MQ=255) tAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGa < 1:2053553/71‑1 (MQ=255) cgcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATc > 1:3552393/1‑50 (MQ=255) gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTa > 1:36356/1‑56 (MQ=255) gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGAt > 1:2871749/1‑71 (MQ=255) tCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTc > 1:1952030/1‑70 (MQ=255) tCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCa < 1:2082838/71‑1 (MQ=255) aGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCaa > 1:1435046/1‑70 (MQ=255) aGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCaa > 1:3271401/1‑70 (MQ=255) aGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCaa > 1:2836133/1‑70 (MQ=255) aTTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACACACGCa > 1:692061/1‑55 (MQ=255) aTTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGt > 1:2046440/1‑71 (MQ=255) ttGAGCACCTAATGCCGGATGCGTCGTGAACGCCtt < 1:2279280/36‑1 (MQ=255) tGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTg > 1:1093660/1‑71 (MQ=255) tGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAAGCACGAAGATTCAAc > 1:1468535/1‑65 (MQ=255) gCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCaa > 1:506707/1‑71 (MQ=255) cACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCaa > 1:868365/1‑70 (MQ=255) aCCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCaa > 1:1642281/1‑69 (MQ=255) tAATGACGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGAt > 1:3340311/1‑71 (MQ=255) aTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGa > 1:3070173/1‑49 (MQ=255) | TCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGAT > minE/2013405‑2013536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |