| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,445 | T→C | 6.8% | S476S (TCA→TCG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,445 | 0 | T | C | 6.8% | 293.6 / 9.5 | 103 | S476S (TCA→TCG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (42/54); new base C (5/2); total (47/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTA > minE/311375‑311512 | tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCt < 1:2161789/71‑1 (MQ=255)tCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCt < 1:3442868/71‑1 (MQ=255) ggTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTg > 1:489538/1‑70 (MQ=255) ggTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTg < 1:3007716/70‑1 (MQ=255) ggTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTg > 1:695629/1‑70 (MQ=255) tCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAAc > 1:1477594/1‑70 (MQ=255) tCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAAc > 1:70601/1‑70 (MQ=255) cAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAAc < 1:2598242/69‑1 (MQ=255) aGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACaa < 1:3951790/70‑1 (MQ=255) gCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATg > 1:3808214/1‑71 (MQ=255) gCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATg > 1:2058163/1‑71 (MQ=255) cATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATgg > 1:593833/1‑71 (MQ=255) gtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTaa < 1:3640436/70‑1 (MQ=255) tggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTg < 1:3794530/57‑1 (MQ=255) tggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTaa > 1:1303770/1‑69 (MQ=255) cacaAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCt > 1:1193957/1‑53 (MQ=255) cacaAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATagag < 1:1996410/71‑1 (MQ=255) cacaAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATagag < 1:899535/71‑1 (MQ=255) aaaGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGaa < 1:1873868/53‑1 (MQ=255) aaaGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAAc < 1:2336385/71‑1 (MQ=255) agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATaga > 1:419918/1‑65 (MQ=255) agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACt > 1:1855982/1‑70 (MQ=255) agCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATagag < 1:839772/64‑1 (MQ=255) agCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTg < 1:2537523/69‑1 (MQ=255) agCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTg < 1:2949462/69‑1 (MQ=255) agCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTg < 1:1946986/69‑1 (MQ=255) gCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACt < 1:3460058/67‑1 (MQ=255) gCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACg < 1:1926824/71‑1 (MQ=255) tGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACga < 1:1500303/70‑1 (MQ=255) gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgata < 1:3904902/70‑1 (MQ=255) gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgata < 1:849824/70‑1 (MQ=255) aGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgataga < 1:1010855/71‑1 (MQ=255) gTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgataga > 1:1811894/1‑70 (MQ=255) tCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATagag < 1:3379031/56‑1 (MQ=255) cAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGatagatag > 1:2339641/1‑71 (MQ=255) agaaTCAATGCCACCAGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCgataaat > 1:1257911/4‑68 (MQ=9) aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATaga > 1:296897/1‑53 (MQ=255) aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATag > 1:3913053/1‑52 (MQ=255) aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatag < 1:3707073/66‑1 (MQ=255) ggATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAAt < 1:1996983/49‑1 (MQ=255) gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACaa > 1:3302518/1‑41 (MQ=255) gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACaa < 1:797167/41‑1 (MQ=255) gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:2687185/1‑71 (MQ=255) aaTCAATGCCACCAGAACAGAAAGCGCCATTGCCGAAACGATgg > 1:3952667/2‑44 (MQ=16) aaTCAATGCCACCAGAACAGAAAGCGCCATTGCCGAAACGATgg > 1:175887/2‑44 (MQ=16) tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatagat > 1:501102/1‑64 (MQ=255) aCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATagag < 1:3548751/47‑1 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg > 1:2204283/1‑68 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg > 1:1344455/1‑68 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg > 1:2798622/1‑68 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:3281954/71‑1 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:3868617/71‑1 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATagag > 1:1944647/1‑45 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:1353623/1‑64 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg > 1:247677/1‑70 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg > 1:2438780/1‑70 (MQ=255) gCCACCAGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCgataaatt < 1:414019/60‑2 (MQ=9) ccaccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa < 1:2670857/71‑1 (MQ=255) caccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACg < 1:3768344/51‑1 (MQ=255) accaGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAAcc > 1:666546/1‑71 (MQ=12) ccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatagat < 1:1466559/55‑1 (MQ=255) caGTACCGACAGCGCCATTGCTGAAACAATGGTAATagag < 1:741448/40‑1 (MQ=255) caGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgataga > 1:222947/1‑53 (MQ=255) caGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatag < 1:332227/52‑1 (MQ=255) caGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatag < 1:3684198/52‑1 (MQ=255) caGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGc > 1:2718405/1‑71 (MQ=255) gTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgat > 1:619169/1‑48 (MQ=255) tACCGACAGCGCCATTGCTGAAACAATGGTAATag < 1:1039896/35‑1 (MQ=255) tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgata < 1:3428857/48‑1 (MQ=255) tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgata < 1:1095058/48‑1 (MQ=255) tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa > 1:117680/1‑70 (MQ=255) tACCGACAGCGCCATT‑CTGAAACAATGGTAATAGAGAACTg > 1:2981559/1‑41 (MQ=25) aCCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa > 1:2159153/1‑69 (MQ=255) aCCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa > 1:2570316/1‑69 (MQ=255) ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:1279674/71‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:454905/71‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:1460748/71‑1 (MQ=255) gACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg > 1:546196/1‑55 (MQ=255) gACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa < 1:3497770/71‑1 (MQ=255) gcgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGc < 1:3260098/70‑1 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCa > 1:2331992/1‑46 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAAcc < 1:630117/57‑1 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:1819940/1‑66 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:2682644/1‑66 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCa < 1:2867743/71‑1 (MQ=255) gcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAt < 1:3408709/71‑1 (MQ=255) gcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCAt < 1:2113064/71‑1 (MQ=9) ccATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTa > 1:2686190/1‑50 (MQ=255) cATTGCTGAAACAATGGTAATAGAGTACTGACGATAGATAGCACCAGTAGAAcc < 1:2778342/54‑1 (MQ=255) cATTGCTGAAACAATGGTAATAGAGAACTGACgata < 1:3806054/36‑1 (MQ=255) cATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCg > 1:2119889/1‑71 (MQ=255) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:1450810/71‑1 (MQ=255) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:3003775/71‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:414223/1‑70 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:3866116/1‑70 (MQ=255) tGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa > 1:3122340/1‑71 (MQ=255) tGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCGTCggaa > 1:3936622/1‑69 (MQ=9) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:556139/57‑1 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:2815359/57‑1 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag < 1:1593080/57‑1 (MQ=255) | TCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTA > minE/311375‑311512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |