Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,035,931	C→T	10.7%	F21F (TTC→TTT)	ygiC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,035,931	0	C	T	10.7%	180.2 / 10.2	65	F21F (TTC→TTT)	ygiC	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/29);  new base T (4/3);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

CGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCC  >  minE/2035867‑2035997                                                                 |                                                                   cGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAAtttt                                                              >  1:1653067/1‑71 (MQ=255)      gAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAAtt                                                                >  1:3508347/1‑64 (MQ=255)      gAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCacac                                                         >  1:1707466/1‑71 (MQ=255)       aaaGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACAcc                                                        <  1:3192899/71‑1 (MQ=255)       aaaGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACAcc                                                        <  1:3794788/71‑1 (MQ=255)       aaaGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACAcc                                                        <  1:1518354/71‑1 (MQ=255)         agagTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTTAATTTTCACACCAt                                                      >  1:89825/1‑71 (MQ=255)         agagTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCAt                                                      >  1:1716238/1‑71 (MQ=255)          gagTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATg                                                     <  1:2503556/71‑1 (MQ=255)            gTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTa                                                   <  1:2239589/71‑1 (MQ=255)             tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTTAATTTTCACACCATGTa                                                   <  1:519012/70‑1 (MQ=255)             tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCacac                                                         <  1:1378150/64‑1 (MQ=255)              cAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGt                                                    >  1:709130/1‑68 (MQ=255)               aGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTTAATTTTCACACCATGTAcag                                                >  1:2058526/1‑69 (MQ=255)               aGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACgg                                                >  1:2321233/1‑71 (MQ=255)               aGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACg                                                 >  1:1528494/1‑70 (MQ=255)                 tATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGc                                               >  1:2612054/1‑70 (MQ=255)                 tATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCg                                              <  1:437541/71‑1 (MQ=255)                 tATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCg                                              <  1:3871845/71‑1 (MQ=255)                   ttACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAg                                            >  1:2493482/1‑71 (MQ=255)                     aCCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGcc                                          >  1:809693/1‑71 (MQ=255)                      ccGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTTAAtt                                                                <  1:1514175/48‑1 (MQ=255)                      ccGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCg                                         <  1:701151/71‑1 (MQ=255)                        gAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa                                       >  1:2859911/1‑71 (MQ=255)                          gcgcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCaa                                                                  <  1:2007069/42‑1 (MQ=255)                          gcgcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACt                                     <  1:350626/71‑1 (MQ=255)                           cgcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTg                                    <  1:2228277/71‑1 (MQ=255)                            gcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAAttt                                                               <  1:1458672/43‑1 (MQ=255)                               cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCacac                                                         <  1:3519362/46‑1 (MQ=255)                               cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCg                                         <  1:1697571/62‑1 (MQ=255)                               cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGgtgt                                <  1:737561/71‑1 (MQ=255)                                ggACTGGCGTGAGAAAGCCCACGAATACGGTTTTAATTTTCACACCATGTACGGCGAGCCGTACTGgtgt                                >  1:714688/1‑70 (MQ=255)                                ggACTGGCGTGAGAAAGCCCACGAATACGGTTTTAATTTTCACACCATGTACGGCGAGCCGTACTGGtgtg                               <  1:1194143/71‑1 (MQ=255)                                 gACTGGCGTGAGAAAGCCCACGAATACGGTTTCAAtt                                                                >  1:61230/1‑37 (MQ=255)                                 gACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa                                       >  1:40510/1‑62 (MQ=255)                                   cTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTc                                                             <  1:2316118/38‑1 (MQ=255)                                      gCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAg                            >  1:2164402/1‑68 (MQ=255)                                      gCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGa                           <  1:528449/69‑1 (MQ=255)                                        gTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATg                                                     <  1:3917446/41‑1 (MQ=255)                                        gTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGgt                                  >  1:256566/1‑60 (MQ=255)                                          gagaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGa                           >  1:3492253/1‑65 (MQ=255)                                          gagaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCtt                      >  1:2169373/1‑70 (MQ=255)                                            gaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGa                                             >  1:1926371/1‑45 (MQ=255)                                            gaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACt                                     <  1:1714726/53‑1 (MQ=255)                                              aaGCCCACGAATACGGTTTCAATTTTCACACCATGTATGGCGAGCCGTACTGGTGTGAAGATGCTtactac                 <  1:3333482/71‑1 (MQ=255)                                              aaGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTta                     <  1:1745081/67‑1 (MQ=255)                                               aGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTtactac                 >  1:2337832/1‑70 (MQ=255)                                                 cccACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAg              <  1:1616559/71‑1 (MQ=255)                                                   cACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGtt            <  1:3581375/71‑1 (MQ=255)                                                   cACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGtt            <  1:3378722/71‑1 (MQ=255)                                                    aCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa                                       >  1:429680/1‑43 (MQ=255)                                                    aCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa                                       >  1:3252022/1‑43 (MQ=255)                                                    aCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGtt            >  1:3031733/1‑70 (MQ=255)                                                    aCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGtt            >  1:1567025/1‑70 (MQ=255)                                                    aCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGtt            >  1:668717/1‑70 (MQ=255)                                                      gAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGAc         >  1:1481526/1‑71 (MQ=255)                                                       aaTACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAg              >  1:844965/1‑65 (MQ=255)                                                       aaTACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGAcc        >  1:55826/1‑71 (MQ=255)                                                          aCGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTc     >  1:1142349/1‑71 (MQ=255)                                                           cGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTc     <  1:3366082/70‑1 (MQ=255)                                                           cGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTc     <  1:3744069/70‑1 (MQ=255)                                                            ggTTTTAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGc   >  1:713678/1‑71 (MQ=255)                                                             gTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTtactac                 <  1:2574913/56‑1 (MQ=255)                                                             gTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGcc  <  1:2128082/71‑1 (MQ=255)                                                                tCAATTTTCACACCATGTACGGCGAGCCGTACTGgt                                  >  1:2526313/1‑36 (MQ=255)                                                                 |                                                                   CGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCC  >  minE/2035867‑2035997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑