| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,040,951 | T→A | 38.3% | *120K (TAA→AAA) | yqiH → | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,040,951 | 0 | T | A | 38.3% | 28.5 / 51.2 | 47 | *120K (TAA→AAA) | yqiH | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base T (14/15); new base A (13/5); total (27/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.74e-01 | |||||||||||
AAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAA > minE/2040884‑2041017 | aaaaaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCaaaa > 1:1455985/1‑71 (MQ=255) aaaaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCaaaa > 1:2107142/1‑70 (MQ=255) aaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCaaaa > 1:832095/1‑68 (MQ=255) gCTCAACTTCCTAAAGACAGAGAACCGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATa > 1:2369779/1‑70 (MQ=255) gCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATa > 1:2219614/1‑70 (MQ=255) cTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaa < 1:1359614/70‑1 (MQ=255) cAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaa > 1:3700664/1‑70 (MQ=255) aaCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaa > 1:3910544/1‑70 (MQ=255) aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATaaaaaa > 1:3836165/1‑70 (MQ=255) aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaaa < 1:570795/71‑1 (MQ=255) aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaa > 1:3658399/1‑70 (MQ=255) aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaa > 1:1844045/1‑68 (MQ=255) cTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATaaaaaaaa > 1:3533897/1‑71 (MQ=255) ccTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAAtgt > 1:3512989/1‑71 (MQ=255) ccTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAAtg < 1:923492/70‑1 (MQ=255) aGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTaa < 1:567901/49‑1 (MQ=255) aGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTaa < 1:1976690/49‑1 (MQ=255) gagaAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGt < 1:561721/70‑1 (MQ=255) gagaAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGt > 1:3684748/1‑70 (MQ=255) agaAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCa > 1:2330655/1‑66 (MQ=255) gaAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCAc < 1:1825604/71‑1 (MQ=255) aaaCGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTaa < 1:2865738/41‑1 (MQ=255) cGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATg < 1:3361794/71‑1 (MQ=255) gCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATg > 1:2088619/1‑70 (MQ=255) gCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATg > 1:1078297/1‑70 (MQ=255) ctATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:3289688/1‑62 (MQ=255) ctATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTAt > 1:2888146/1‑63 (MQ=255) ctATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCAc < 1:1061969/70‑1 (MQ=255) ctATTTTAACGTGCGAGAAATACCGCCCACAACAGATAAAAAAAATGTGATg > 1:610022/1‑52 (MQ=255) ctATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACg > 1:981902/1‑71 (MQ=255) ttttAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGc < 1:3140684/69‑1 (MQ=255) ttAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTAt > 1:3927522/1‑58 (MQ=255) aaCGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGa < 1:3888023/70‑1 (MQ=255) tGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATg < 1:1253610/53‑1 (MQ=255) cGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATgca > 1:208727/1‑53 (MQ=255) gagaAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTc < 1:2778175/71‑1 (MQ=255) gagaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCaa > 1:2287940/1‑41 (MQ=255) gaAATACCGCCCTAAACAGATAAAAAAAATGTGATg > 1:3459798/1‑36 (MQ=255) gaAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCt > 1:2046976/1‑70 (MQ=255) gaAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTg < 1:3093866/71‑1 (MQ=255) gaAATACCGCCCAAAACAGATAAAAAAAATGTGAt < 1:3771182/35‑1 (MQ=255) aaaTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCAc < 1:2638720/43‑1 (MQ=255) aaaTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCAc < 1:2167216/43‑1 (MQ=255) aaaTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTgg > 1:698145/1‑71 (MQ=255) aaaTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGAAGCACGCATTGAAACTATTCTg > 1:532268/1‑70 (MQ=255) aaaTACCGCCCAAAACAGATAAAAAAAATGTGATGCaa > 1:20609/1‑38 (MQ=255) aaaTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTgg > 1:3825791/1‑71 (MQ=255) aaaTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTgg > 1:3691071/1‑71 (MQ=255) aTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTggcg < 1:234308/71‑1 (MQ=255) gCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCaa < 1:2581458/71‑1 (MQ=255) tAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTgg > 1:1543973/1‑60 (MQ=255) | AAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAA > minE/2040884‑2041017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |