| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,041,438 | G→A | 46.5% | D32N (GAT→AAT) | yqiI → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,041,438 | 0 | G | A | 46.5% | 18.5 / 112.4 | 86 | D32N (GAT→AAT) | yqiI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (25/21); new base A (22/18); total (47/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCT > minE/2041371‑2041507 | tttttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATa > 1:1152225/1‑71 (MQ=255) ttttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGgag > 1:2628754/1‑67 (MQ=255) tttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATAcc > 1:3856355/1‑71 (MQ=255) tttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATAcc > 1:3634438/1‑71 (MQ=255) tttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGt > 1:1280694/1‑71 (MQ=255) tATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGtt < 1:562597/70‑1 (MQ=255) aTGGGTTTTAGTTCATTAGCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTg < 1:871471/70‑1 (MQ=255) tGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATAACGTTGt > 1:1600578/1‑70 (MQ=255) tGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGt > 1:2265995/1‑70 (MQ=255) tGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGt > 1:2261417/1‑70 (MQ=255) gggTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCa > 1:527098/1‑71 (MQ=255) ggTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCa < 1:3033181/70‑1 (MQ=255) ggTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCAt < 1:605852/71‑1 (MQ=255) ggTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCAt < 1:3412174/71‑1 (MQ=255) gTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCAtt < 1:289488/71‑1 (MQ=255) gTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCAtt > 1:1419466/1‑71 (MQ=255) ttttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTg > 1:1836003/1‑71 (MQ=255) tttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTgg > 1:132476/1‑71 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt > 1:1437825/1‑71 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt > 1:608665/1‑71 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt > 1:3925757/1‑71 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt > 1:1525930/1‑71 (MQ=255) tAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt < 1:2127047/70‑1 (MQ=255) tAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt < 1:3502539/70‑1 (MQ=255) aGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGtt < 1:492131/70‑1 (MQ=255) aGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGtt < 1:2030411/70‑1 (MQ=255) aTTACCTGCATGGGCAATGGACTGCTATGCTGAACTTGAAGGTGGAGATACCGTTGTCATTGGTTACGTa < 1:3772311/70‑1 (MQ=255) aTTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt < 1:2131327/64‑1 (MQ=255) ttACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTAc > 1:2842848/1‑70 (MQ=255) ttACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTAc > 1:2532754/1‑70 (MQ=255) tACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTAcc > 1:2792558/1‑70 (MQ=255) aCCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCa > 1:3005392/1‑56 (MQ=255) aCCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTAcc > 1:686378/1‑69 (MQ=255) aCCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTAcc > 1:134886/1‑69 (MQ=255) ccTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAg < 1:46732/71‑1 (MQ=255) ccTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTgg < 1:861549/59‑1 (MQ=255) cTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATa > 1:1014787/1‑45 (MQ=255) gCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTAcc < 1:1697998/65‑1 (MQ=255) cATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGt < 1:3140066/46‑1 (MQ=255) aTGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAAtct < 1:943643/71‑1 (MQ=255) tGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATAcc < 1:3721910/42‑1 (MQ=255) ggCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATctct < 1:3449138/70‑1 (MQ=255) ggCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATctct < 1:603309/70‑1 (MQ=255) ggCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTa < 1:3283070/71‑1 (MQ=255) gCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTAt > 1:3909506/1‑71 (MQ=255) cAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTg < 1:3209902/42‑1 (MQ=255) cAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTgg < 1:2408612/49‑1 (MQ=255) cAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATc < 1:2703489/71‑1 (MQ=255) aaTGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCTAGAATCTCTATcc > 1:3509715/1‑71 (MQ=255) aTGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCaa > 1:104708/1‑58 (MQ=255) aTGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATccc < 1:3195374/71‑1 (MQ=255) aTGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATccc > 1:1138596/1‑71 (MQ=255) gACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt > 1:2102183/1‑45 (MQ=255) gACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt > 1:3870836/1‑45 (MQ=255) gACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt > 1:3632393/1‑45 (MQ=255) tGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGa > 1:1782755/1‑71 (MQ=255) gCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGtt > 1:2216529/1‑42 (MQ=255) cTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGa < 1:3829628/69‑1 (MQ=255) aTGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATgg > 1:3085961/1‑70 (MQ=255) aTGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATgg > 1:2270215/1‑70 (MQ=255) tGCTGAACATGAAGGTGGAGATTCCGTTGTCATTGGTTACGTACCAAGa < 1:1636178/49‑1 (MQ=255) gCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATg > 1:526369/1‑67 (MQ=255) cTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaa < 1:1889787/70‑1 (MQ=255) cTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaa > 1:1492351/1‑70 (MQ=255) cTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaa < 1:2057175/71‑1 (MQ=255) cTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaa > 1:917171/1‑70 (MQ=255) tGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACCTACCaa < 1:506919/44‑1 (MQ=38) gAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAAtctc < 1:2604695/50‑1 (MQ=255) gAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAAtctc < 1:3951285/50‑1 (MQ=255) gAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCa < 1:41711/58‑1 (MQ=255) gAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGa < 1:1154296/62‑1 (MQ=255) aaCATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaaa < 1:1487657/70‑1 (MQ=255) aaCATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaaa < 1:2967618/70‑1 (MQ=255) aaCATCAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAAtct < 1:86977/48‑1 (MQ=38) aCATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGa > 1:3229302/1‑43 (MQ=255) aCATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGAt > 1:3854631/1‑61 (MQ=255) cATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAgg > 1:1129933/1‑71 (MQ=255) cATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAgg > 1:1132380/1‑71 (MQ=255) cATGAAGGTGGAAATACCGTTGTCATTGGTTACGTa < 1:2317758/36‑1 (MQ=255) cATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAAtc > 1:2483257/1‑45 (MQ=255) gAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGa > 1:1555784/1‑71 (MQ=255) aGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAgg > 1:3574113/1‑66 (MQ=255) aGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGAt > 1:1507217/1‑70 (MQ=255) gTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCCATGGTAAAAAAGGTGATaaa < 1:1296466/71‑1 (MQ=255) tctagaTACCGTTGTCATTGGTTACGTAACAAGAATCTCTATCCCCAGAGATGGTAAAAAAGGTGATaaa < 1:2572316/67‑1 (MQ=255) ggAAATACCGTTGTCATTGGTTACGTACCAAGAAtc > 1:3409459/1‑36 (MQ=255) ggAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATg > 1:118906/1‑52 (MQ=255) ggAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAAt > 1:1239760/1‑71 (MQ=255) agaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGa < 1:2675063/48‑1 (MQ=255) agaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCt < 1:443582/71‑1 (MQ=255) | TTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCT > minE/2041371‑2041507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |