| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,045,835 | C→T | 7.3% | intergenic (+597/+198) | yqiK → / ← rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,045,835 | 0 | C | T | 7.3% | 214.1 / 4.9 | 68 | intergenic (+597/+198) | yqiK/rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (26/37); new base T (2/3); total (28/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
TCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGA > minE/2045766‑2045905 | gccGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCt < 1:753594/70‑1 (MQ=255) cgcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtat < 1:2460908/71‑1 (MQ=255) cgcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGta > 1:934496/1‑70 (MQ=255) gcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtata < 1:1240967/71‑1 (MQ=255) gcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtata < 1:893500/71‑1 (MQ=255) tcatcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtat < 1:3810546/68‑1 (MQ=255) atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGta > 1:299217/1‑65 (MQ=255) atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAgg > 1:1848227/1‑70 (MQ=255) caacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGa > 1:383422/1‑69 (MQ=255) gggggCAGCAAGGGGCGGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCtt < 1:2321566/51‑1 (MQ=255) ggggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGtat < 1:379648/54‑1 (MQ=255) ggggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTatgat < 1:2822319/71‑1 (MQ=255) ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTAtgatga < 1:2250737/71‑1 (MQ=255) gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCt > 1:3826505/1‑71 (MQ=255) caAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAg > 1:1959571/1‑68 (MQ=255) aaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGaaa > 1:2838940/1‑53 (MQ=255) aaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg > 1:1376259/1‑71 (MQ=255) acgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGt > 1:1708430/3‑70 (MQ=0) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat < 1:1936945/71‑1 (MQ=255) gCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTatgatg < 1:3100332/59‑1 (MQ=255) tGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg < 1:748236/64‑1 (MQ=255) gAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAgg > 1:3395419/1‑47 (MQ=255) aaaCGTGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:3550215/69‑1 (MQ=255) aaaCGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTAtgatga > 1:116466/1‑57 (MQ=0) aaaCGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:3128594/69‑1 (MQ=11) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGaaa < 1:2098056/44‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTat < 1:3155389/51‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:2923315/69‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc > 1:673150/1‑71 (MQ=255) aaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 1:2633942/70‑1 (MQ=255) aCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTTTATAAg > 1:3420149/1‑38 (MQ=255) aCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc > 1:3282549/1‑59 (MQ=255) aCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc > 1:2261239/1‑59 (MQ=255) gggAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTgg < 1:3376571/71‑1 (MQ=11) gggAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTgg < 1:1049747/71‑1 (MQ=11) ggAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTa < 1:3541859/46‑1 (MQ=0) gAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg > 1:2125140/1‑71 (MQ=255) aaaGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgt < 1:484967/71‑1 (MQ=255) aGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatc < 1:1334196/61‑2 (MQ=9) cccTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatcg < 1:994508/59‑3 (MQ=255) cccTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAg < 1:273649/46‑1 (MQ=255) cccTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt < 1:3639705/71‑1 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgt > 1:1881117/1‑66 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa < 1:3464314/71‑1 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa > 1:1424878/1‑71 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa > 1:3654444/1‑71 (MQ=255) cccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt > 1:897779/1‑71 (MQ=255) ccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTatgat > 1:3361621/1‑37 (MQ=255) ccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGt > 1:11342/1‑46 (MQ=255) ccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGt > 1:1171079/1‑46 (MQ=255) ccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTg < 1:1935560/71‑1 (MQ=255) cGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGt < 1:2794176/71‑1 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:2451304/50‑1 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:2934175/50‑1 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 1:321771/71‑1 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt > 1:3731076/1‑70 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt > 1:2619352/1‑70 (MQ=255) agaag‑GGGCCTTGTATAAGGAAAGGTTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGt > 1:2305512/1‑68 (MQ=255) gaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 1:1890929/68‑1 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgt > 1:2667839/1‑55 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgt > 1:2122189/1‑55 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtg < 1:3131370/54‑1 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtg < 1:1989837/54‑1 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt < 1:881928/71‑1 (MQ=255) ggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTgttgtt < 1:2941019/57‑1 (MQ=255) ggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTgttgtt < 1:3154011/57‑1 (MQ=255) ggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTg < 1:3865645/61‑1 (MQ=255) gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc < 1:2440058/71‑1 (MQ=255) ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTTCTGTTAAGTTTccc > 1:3193328/1‑70 (MQ=255) ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCg < 1:2919349/71‑1 (MQ=255) ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGATTccc > 1:595478/1‑70 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt < 1:3029819/53‑1 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa < 1:1786667/71‑1 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:1380385/1‑71 (MQ=255) | TCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGA > minE/2045766‑2045905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |