| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,045,864 | T→A | 6.6% | intergenic (+626/+169) | yqiK → / ← rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,045,864 | 0 | T | A | 6.6% | 240.5 / 6.7 | 88 | intergenic (+626/+169) | yqiK/rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (31/51); new base A (2/4); total (33/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGG > minE/2045794‑2045930 | gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCt > 1:3826505/1‑71 (MQ=255) aaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg > 1:1376259/1‑71 (MQ=255) acgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGt > 1:1708430/3‑70 (MQ=0) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat < 1:1936945/71‑1 (MQ=255) tGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg < 1:748236/64‑1 (MQ=255) aaaCGTGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:3550215/69‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:3128594/69‑1 (MQ=11) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:2923315/69‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc > 1:673150/1‑71 (MQ=255) aaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc < 1:2633942/70‑1 (MQ=255) aCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc > 1:2261239/1‑59 (MQ=255) aCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc > 1:3282549/1‑59 (MQ=255) gggAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTgg < 1:1049747/71‑1 (MQ=11) gggAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTgg < 1:3376571/71‑1 (MQ=11) gAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg > 1:2125140/1‑71 (MQ=255) aaaGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgt < 1:484967/71‑1 (MQ=255) aGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatc < 1:1334196/61‑2 (MQ=9) cccTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatcg < 1:994508/59‑3 (MQ=255) cccTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt < 1:3639705/71‑1 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgt > 1:1881117/1‑66 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa < 1:3464314/71‑1 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa > 1:3654444/1‑71 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa > 1:1424878/1‑71 (MQ=255) cccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt > 1:897779/1‑71 (MQ=255) ccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGt > 1:11342/1‑46 (MQ=255) ccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGt > 1:1171079/1‑46 (MQ=255) ccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTg < 1:1935560/71‑1 (MQ=255) cGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGt < 1:2794176/71‑1 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:2934175/50‑1 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat < 1:2451304/50‑1 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 1:321771/71‑1 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt > 1:3731076/1‑70 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt > 1:2619352/1‑70 (MQ=255) agaag‑GGGCCTTGTATAAGGAAAGGTTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGt > 1:2305512/1‑68 (MQ=255) gaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 1:1890929/68‑1 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgt > 1:2122189/1‑55 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgt > 1:2667839/1‑55 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtg < 1:3131370/54‑1 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtg < 1:1989837/54‑1 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt < 1:881928/71‑1 (MQ=255) ggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTgttgtt < 1:3154011/57‑1 (MQ=255) ggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTgttgtt < 1:2941019/57‑1 (MQ=255) ggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTg < 1:3865645/61‑1 (MQ=255) gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc < 1:2440058/71‑1 (MQ=255) ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTTCTGTTAAGTTTccc > 1:3193328/1‑70 (MQ=255) ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCg < 1:2919349/71‑1 (MQ=255) ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGATTccc > 1:595478/1‑70 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt < 1:3029819/53‑1 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:1380385/1‑71 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa < 1:1786667/71‑1 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa < 1:1747711/68‑1 (MQ=255) tataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGtt < 1:1645400/61‑1 (MQ=255) aataAGGAAAGGGTTATGATGAAGCACGTCATCATAc > 1:3496380/2‑37 (MQ=11) aataAGGAAAGGGTTATGATGAAGCACGTCATCATACTg < 1:1770919/38‑1 (MQ=11) ataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGAc < 1:1473262/67‑1 (MQ=255) ataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGAc < 1:3658789/67‑1 (MQ=255) ataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGAc < 1:2119217/67‑1 (MQ=255) taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg < 1:2250128/37‑1 (MQ=33) taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:3412248/1‑65 (MQ=255) aGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg < 1:594639/39‑1 (MQ=40) aGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTc > 1:2682945/1‑59 (MQ=255) ggAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCg < 1:1113711/61‑1 (MQ=255) ggAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCg < 1:3240690/61‑1 (MQ=255) gAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTTTGTTGTTACTGTTAAGTTTCCCGACTTACTaaca < 1:1091101/71‑1 (MQ=255) aaaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTg > 1:954642/1‑47 (MQ=40) taGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt > 1:1094889/2‑48 (MQ=255) aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt > 1:3733424/1‑48 (MQ=255) ttATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa < 1:935167/44‑1 (MQ=255) tATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 1:153998/42‑1 (MQ=255) atgatgAAGCTCGTCATCATACTGGTTGTgttgtt < 1:2637366/35‑1 (MQ=40) atgatgAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTa > 1:3399207/1‑56 (MQ=255) tgatgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCag < 1:3218432/70‑1 (MQ=255) gatgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt < 1:3270620/45‑1 (MQ=255) atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGt < 1:26256/42‑1 (MQ=255) atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGt < 1:991015/42‑1 (MQ=255) atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaa > 1:2594083/1‑57 (MQ=255) atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaa > 1:3045629/1‑57 (MQ=255) atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAgg < 1:364460/71‑1 (MQ=255) tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTTTTAAGTTTCCCGACTTACTAACAACTCATCAGAggg < 1:732493/71‑1 (MQ=255) tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa > 1:3811719/1‑39 (MQ=255) tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggg < 1:3064798/71‑1 (MQ=255) gaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa > 1:393412/1‑58 (MQ=255) gaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa > 1:221587/1‑58 (MQ=255) aGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTc > 1:1955551/1‑41 (MQ=255) aGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggggg < 1:3009946/70‑1 (MQ=255) aGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggggg < 1:3932634/70‑1 (MQ=255) aGCTCGTCATCATACTGGTTGAGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggggg < 1:458582/70‑1 (MQ=255) aGCACGTCATCATACTGGTGATACTCTTAGTGATTAGCTTCCAGGCTTACTaag < 1:1602202/54‑2 (MQ=255) cTCGTCATCATACTGGGTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACt < 1:3433658/56‑1 (MQ=255) | GCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGG > minE/2045794‑2045930 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |